Bioencapsulation of Oocytes and Granulosa Cells

2018 ◽  
pp. 89-93
Author(s):  
Massimo Faustini ◽  
Giulio Curone ◽  
Maria L. Torre ◽  
Daniele Vigo
Keyword(s):  
Granulosa Cells

Importance of the Renin-Angiotensin-System in human granulosa lutein cells – RAS in granulosa cells

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
D Herr ◽  
WC Duncan ◽  
G Hack ◽  
R Konrad ◽  
R Kreienberg ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin

Butyryl- and acetylcholine-esterases in human IVF-derived follicular fluid and luteinizing granulosa cells

2013 ◽  
Vol 121 (03) ◽  
Author(s):  
J Blohberger ◽  
D Einwang ◽  
D Berg ◽  
U Berg ◽  
S Hecht ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Human Ivf

Corticotrophin-Releasing-Hormone (CRH) modulates ovarian steroid biosynthesis in human primary granulosa cells

2016 ◽  
Vol 76 (10) ◽  
Author(s):  
S Heublein ◽  
S Hecht ◽  
V Nick ◽  
S Mahner ◽  
C Thaler ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Steroid Biosynthesis ◽  
Ovarian Steroid ◽  
Releasing Hormone

Regulatory effect of vasopressin and its analogs on the steroidogenesis of human granulosa cells (GC) in vitro

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S183-S185
Author(s):  
H. MUELLER ◽  
T. RABE ◽  
B. HAUFF ◽  
L. KIESEL ◽  
B. RUNNEBAUM
Keyword(s):  
Granulosa Cells ◽  
Regulatory Effect ◽  
Human Granulosa Cells

A technique for superfusion of isolated granulosa cells. Dynamics of cAMP production and steroidogenesis in response to gonadotropins

1988 ◽  
Vol 117 (4) ◽  
pp. 497-506 ◽  
Author(s):  
Carl Johanson ◽  
Viktor Johanson
Keyword(s):  
Granulosa Cells ◽  
Culture Medium ◽  
Cell Damage ◽  
Adenosine Monophosphate ◽  
Camp Production ◽  
Morphological Examination ◽  
Ovarian Cells ◽  
Temporal Relationships ◽  
Polycarbonate Membranes ◽  
Estradiol 17Β

Abstract. A superfusion model for isolated ovarian cells was developed and characterized in detail. Granulosa cells isolated from pre-ovulatory rat ovarian follicles were placed in superfusion (perifusion) chambers with a volume of 125 μl. Culture medium was pumped through the chambers, collected in 20-min fractions of 600 μl and analysed for cAMP and steroids. Viability was confirmed by morphological examination. The use of polycarbonate membranes to retain the cells in the chambers was abandoned since the membranes caused severe cell damage. The temporal relationships between gonadotropic stimuli and the release of cyclic 3':5'-adenosine monophosphate (cAMP) and steroids was investigated. Within 10 min FSH elicited transient increase in the release of cAMP and progesterone but had no effect on testosterone or estradiol-17β release. Amplitude and duration of the response in cAMP and progesterone release were correlated to concentration and length of the FSH pulse when these parameters were varied within the ranges 1–100 μg/l and 30–270 min, respectively. Compared with the cAMP response, the progesterone response peaked up to 30 min later and lasted 1 to 2 h longer but could not be extended to more than approximately 6 h, not even with longer FSH pulses. These results could indicate a development of desensitization.

cDNA cloning reveals the potential of human ovarian granulosa cells to secrete collagenase inhibitor

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S138
Author(s):  
J. FREUDENSTEIN ◽  
J. MUCHA ◽  
G. RAPP ◽  
K. H. SHEIT
Keyword(s):  
Cdna Cloning ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells ◽  
Collagenase Inhibitor
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