Artifacts and Common Errors in Protein Gel Electrophoresis

2018 ◽  
pp. 511-518
Author(s):  
Biji T. Kurien ◽  
R. Hal Scofield
Keyword(s):  
Gel Electrophoresis ◽  
Protein Gel

User-friendly tool kits for protein gel electrophoresis techniques: A training program for high school students

2018 ◽  
Vol 46 (5) ◽  
pp. 566-577 ◽  
Author(s):  
Praveen Maurye ◽  
Arpita Basu ◽  
Sohini Sen ◽  
Jayanta Kumar Biswas ◽  
Tapas Kumar Bandyopadhyay ◽  
...  
Keyword(s):  
High School ◽  
High School Students ◽  
Training Program ◽  
Gel Electrophoresis ◽  
School Students ◽  
Protein Gel ◽  
User Friendly

Two-dimensional protein gel electrophoresis: A powerful tool in experimental pharmacology and toxicology

1995 ◽  
Vol 31 ◽  
pp. 376
Author(s):  
L. Aicher ◽  
M. Varela ◽  
D. Wahl ◽  
N.L. Anderson ◽  
J.P. Hofmann ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Protein Gel ◽  
Experimental Pharmacology ◽  
Pharmacology And Toxicology

scholarly journals Cold Acclimation and Alterations in Dehydrin-like and Bark Storage Proteins in the Leaves of Sibling Deciduous and Evergreen Peach

1996 ◽  
Vol 121 (5) ◽  
pp. 915-919 ◽  
Author(s):  
Rajeev Arora ◽  
Michael Wisniewski ◽  
Lisa J. Rowland
Keyword(s):  
Cold Acclimation ◽  
Gel Electrophoresis ◽  
Prunus Persica ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Subzero Temperature ◽  
Protein Gel ◽  
Bark Storage Proteins ◽  
High Level

Seasonal changes in cold tolerance and proteins were studied in the leaves of sibling deciduous and evergreen peach [Prunus persica (L.) Batsch]. Freezing tolerance [defined as the subzero temperature at which 50% injury occurred (LT50)] was assessed using electrolyte leakage. Proteins were separated by sodium dodecyl sulfate polyacrylamide-gel electrophoresis. Electroblots were probed with anti-dehydrin and anti-19-kD peach bark storage protein (BSP) antibodies. Leaf LT50 decreased successively from -5.8 °C on 18 Aug. to -10.3 °C in the evergreen genotype and from -7.0 °C to -15.0 °C in the deciduous genotype by 14 Oct. Protein profiles and immunoblots indicated the accumulation of a 60- and 30-kD protein during cold acclimation in the leaves of deciduous trees; however, levels of these proteins did not change significantly in the evergreen trees. Immunoblots indicate that the 60-kD protein is a dehydrin-like protein. Gel-electrophoresis and immunoblots also indicated that the 19-kD BSP progressively disappeared from summer through fall in leaves of deciduous peach but accumulated to a high level in bark tissues. A similar inverse relationship was not evident in evergreen peach.

scholarly journals Discontinuous native protein gel electrophoresis

2006 ◽  
Vol 27 (20) ◽  
pp. 3949-3951 ◽  
Author(s):  
Michael Niepmann ◽  
Junfeng Zheng
Keyword(s):  
Gel Electrophoresis ◽  
Native Protein ◽  
Protein Gel

A rapid antibody-based test for Sec-2, a marker for the short arm of chromosome 2 of rye (2RS)

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1006-1012 ◽  
Author(s):  
J. H. Skerritt ◽  
R. B. Gupta ◽  
J. L. Andrews ◽  
F. L. Stoddard ◽  
N. K. Howes
Keyword(s):  
Gel Electrophoresis ◽  
Seed Proteins ◽  
Antibody Test ◽  
Wheat Chromosome ◽  
Chromosome 2 ◽  
Wheat Seed ◽  
Protein Gel ◽  
Seed Population ◽  
Selection Of ◽  
Rapid Antibody

A simple monoclonal antibody-based screening test has been developed for the presence of translocations of the short arm of chromosome 2 of rye (2RS) with wheat chromosome 2B. 2RS encodes a set of about three polypeptides known as Mr 75 000 gamma-secalins. Use of the antibody test for these secalins enabled screening of several hundred seeds per day. The antibody could readily distinguish 2BL–2RS translocations and 2R substitutions from 1BL–1RS translocations or nontranslocation wheats. Use of the antibody in analysis of segregating progeny for Sec-2 in several wheat backgrounds was successful. Results with a selection of the seed population were checked using protein gel electrophoresis, with 100% correct confirmation. Key words : rye, wheat, seed proteins, translocation, diagnostic test.

scholarly journals Comparison of multi-locus enzyme and protein gel electrophoresis in the discrimination of five Fusarium species isolated from Egyptian cottons

2003 ◽  
Vol 2 (7) ◽  
pp. 206-210 ◽  
Author(s):  
N. Aly Ibrahim ◽  
A. Abdel-Sattar Mohmed ◽  
A. Abd-Elsalam Kamel ◽  
S. Khalil Mohmed ◽  
A. Verreet Joseph
Keyword(s):  
Gel Electrophoresis ◽  
Fusarium Species ◽  
Protein Gel

scholarly journals Biochemical changes in developing embryos of Schistocerca gregaria (Orthoptera: Acrididae) induced by pheromone produced by ovipositing gregarious females

2015 ◽  
Vol 35 (03) ◽  
pp. 125-131
Author(s):  
Fathiya M. Khamis ◽  
Paul O. Mireji ◽  
Ellie O. Osir ◽  
Mabel O. Imbuga ◽  
Ahmed Hassanali
Keyword(s):  
Gel Electrophoresis ◽  
Time Course ◽  
Schistocerca Gregaria ◽  
Two Dimensional ◽  
Protein Patterns ◽  
Gene Expressions ◽  
Protein Gel ◽  
Profile Matching ◽  
Signalling Cascades ◽  
Different Levels

Trans-generational transfer of gregarious-phase traits in the desert locustSchistocerca gregaria(Forskål, 1775) is mediated by primer gregarizing pheromonal signals produced by ovipositing females that experience crowding. We monitored time-course proteomic events in eggs from solitary-reared locusts that had been exposed for 1, 3, 5, 7, 10 and 12 days to different levels of the sand-associated gregarizing signal originating from 0, 3, 5 or 10 ovipositions by crowd-reared females. Evidence for the phase transition was sought by comparing the protein patterns of embryos thus exposed with those from crowd-reared (gregarious) controls; this comparison was continued until the stage of the first instars. Expressed proteins were analysed by two-dimensional protein gel electrophoresis, and patterns from the different treatments within stages were compared by profile matching andχ2analyses. Eggs derived from crowd- and solitary-reared females showed essentially similar protein patterns at early stages of embryogenesis; however, mature stages (particularly, days 10 and 12) and hatchlings demonstrated significantly different patterns. Protein patterns of eggs from solitary-reared females that were incubated in sand contaminated with the pheromonal signal and of the hatchlings that emerged were similar to those derived from gregarious females and dependent on the level of the pheromone to which the embryos had been exposed. The results confirm the gregarizing effect of the signal and constitute a useful basis for unravelling the mechanism of the signalling cascades associated with gene expressions triggered by the pheromone.

2-D Protein Gel Electrophoresis: An Overview

2003 ◽  
pp. 1-8
Author(s):  
Jenny Fichmann ◽  
Reiner Westermeier
Keyword(s):  
Gel Electrophoresis ◽  
Protein Gel
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