A rapid antibody-based test for Sec-2, a marker for the short arm of chromosome 2 of rye (2RS)

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1006-1012 ◽  
Author(s):  
J. H. Skerritt ◽  
R. B. Gupta ◽  
J. L. Andrews ◽  
F. L. Stoddard ◽  
N. K. Howes
Keyword(s):  
Gel Electrophoresis ◽  
Seed Proteins ◽  
Antibody Test ◽  
Wheat Chromosome ◽  
Chromosome 2 ◽  
Wheat Seed ◽  
Protein Gel ◽  
Seed Population ◽  
Selection Of ◽  
Rapid Antibody

A simple monoclonal antibody-based screening test has been developed for the presence of translocations of the short arm of chromosome 2 of rye (2RS) with wheat chromosome 2B. 2RS encodes a set of about three polypeptides known as Mr 75 000 gamma-secalins. Use of the antibody test for these secalins enabled screening of several hundred seeds per day. The antibody could readily distinguish 2BL–2RS translocations and 2R substitutions from 1BL–1RS translocations or nontranslocation wheats. Use of the antibody in analysis of segregating progeny for Sec-2 in several wheat backgrounds was successful. Results with a selection of the seed population were checked using protein gel electrophoresis, with 100% correct confirmation. Key words : rye, wheat, seed proteins, translocation, diagnostic test.

IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

User-friendly tool kits for protein gel electrophoresis techniques: A training program for high school students

2018 ◽  
Vol 46 (5) ◽  
pp. 566-577 ◽  
Author(s):  
Praveen Maurye ◽  
Arpita Basu ◽  
Sohini Sen ◽  
Jayanta Kumar Biswas ◽  
Tapas Kumar Bandyopadhyay ◽  
...  
Keyword(s):  
High School ◽  
High School Students ◽  
Training Program ◽  
Gel Electrophoresis ◽  
School Students ◽  
Protein Gel ◽  
User Friendly

scholarly journals Assessment of the Field Utility of a Rapid Point-of-Care Test for SARS-CoV-2 Antibodies in a Household Cohort

2021 ◽  
Author(s):  
Mehal Churiwal ◽  
Kelly D. Lin ◽  
Salman Khan ◽  
Srijana Chhetri ◽  
Meredith S. Muller ◽  
...  
Keyword(s):  
Cohort Study ◽  
Confidence Interval ◽  
Point Of Care ◽  
Natural Infection ◽  
Field Performance ◽  
Antibody Test ◽  
Asymptomatic Infection ◽  
Point Of Care Test ◽  
Antibody Tests ◽  
Rapid Antibody

Point-of-care (POC) tests to detect SARS-CoV-2 antibodies offer quick assessment of serostatus after natural infection or vaccination. We compared the field performance of the BioMedomics COVID-19 IgM/IgG Rapid Antibody Test against an ELISA in 303 participants enrolled in a SARS-CoV-2 household cohort study. The rapid antibody test was easily implemented with consistent interpretation across 14 users in a variety of field settings. Compared with ELISA, detection of seroconversion lagged by 5 to 10 days. However, it retained a sensitivity of 90% (160/177, 95% confidence interval [CI] 85–94%) and specificity of 100% (43/43, 95% CI 92–100%) for those tested 3 to 5 weeks after symptom onset. Sensitivity was diminished among those with asymptomatic infection (74% [14/19], 95% CI 49–91%) and early in infection (45% [29/64], 95% CI 33–58%). When used appropriately, rapid antibody tests offer a convenient way to detect symptomatic infections during convalescence.

scholarly journals In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum-cephalosporins.

1996 ◽  
Vol 40 (2) ◽  
pp. 342-348 ◽  
Author(s):  
L Martínez-Martínez ◽  
S Hernández-Allés ◽  
S Albertí ◽  
J M Tomás ◽  
V J Benedi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Dna Analysis ◽  
Pulse Field ◽  
Transfer Resistance ◽  
Beta Lactamases ◽  
Pulse Field Gel Electrophoresis ◽  
Gene Coding ◽  
Selection Of

Four Klebsiella pneumoniae isolates (LB1, LB2, LB3, and LB4) with increased antimicrobial resistance were obtained from the same patient. The four isolates were indistinguishable in biotype, plasmid content, lipopolysaccharide, and DNA analysis by pulse-field gel electrophoresis. Isolate LB1 made TEM-1 and SHV-1 beta-lactamases. Isolates LB2, LB3, and LB4 produced SHV-5 in addition to TEM-1 and SHV-1. MICs of cefoxitin, ceftazidime, and cefotaxime against LB1 were 4, 1, and 0.06 micrograms/ml, respectively. MICs of ceftazidime against K. pneumoniae LB2, LB3, and LB4 were > 256 micrograms/ml, and those of cefotaxime were 2, 4, and 64 micrograms/ml, respectively. MICs of cefoxitin against K. pneumoniae LB2 and LB3 were 4 micrograms/ml, but that against K. pneumoniae LB4 was 128 micrgrams/ml. K. pneumoniae LB4 could transfer resistance to ceftazidime and cefotaxime, but not that to cefoxitin, to Escherichia coli. Isolate LB4 and cefoxitin-resistant laboratory mutants lacked an outer membrane protein of about 35 kDa whose molecular mass, mode of isolation, resistance to proteases, and reaction with a porin-specific antiserum suggested that it was a porin. MICs of cefoxitin and cefotaxime reverted to 4 and 2 micrograms/ml, respectively, when isolate LB4 was transformed with a gene coding for the K. pneumoniae porin OmpK36. We conclude that the increased resistance to cefoxitin and expanded-spectrum cephalosporins of isolate LB4 was due to loss of a porin channel for antibiotic uptake.

Identification of an RFLP interval containing Pch2 on chromosome 7AL in wheat

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 249-252 ◽  
Author(s):  
Robert C. de la Peña ◽  
Timothy D. Murray ◽  
Stephen S. Jones
Keyword(s):  
Chinese Spring ◽  
Wheat Chromosome ◽  
Distal Portion ◽  
Rflp Markers ◽  
Substitution Lines ◽  
Eyespot Disease ◽  
Single Marker ◽  
Double Recombination ◽  
Recombinant Substitution Line ◽  
Selection Of

The gene Pch2 in 'Cappelle Desprez' is one of two genes found in hexaploid wheat known to confer resistance to eyespot disease. This study was conducted to develop an RFLP linkage map of the distal portion of wheat chromosome 7AL, and to locate and identify markers closely associated with Pch2 for use in marker-assisted selection. Ten loci in addition to Pch2 were mapped on chromosome 7AL, using segregation data from 102 homozygous chromosome 7A recombinant substitution lines derived from 'Chinese Spring' × 'Chinese Spring' ('Cappelle Desprez' 7A). The Pch2 locus was bracketed by two RFLP markers, one 11.0 cM distal to Xcdo347 and the other 18.8 cM proximal to Xwg380. The position of Pch2 on chromosome 7AL is similar to that of Pch1 on chromosome 7DL, suggesting that these resistance genes are homoeoloci. Although no single marker was closely linked to Pch2, simultaneous selection of the flanking RFLP markers Xcdo347 and Xwg380 could be used for selecting Pch2, since double recombination occurred in only 3% of the recombinant population. The use of the flanking RFLP markers to select for Pch2, in combination with previously identified Pch1-linked markers, would facilitate the development of cultivars carrying two genes for resistance to eyespot.Key words: Triticum aestivum, Pseudocercosporella herpotrichoides, recombinant substitution line.

scholarly journals Performance of the OraQuick HCV Rapid Antibody Test for Screening Exposed Patients in a Hepatitis C Outbreak Investigation

2014 ◽  
Vol 52 (7) ◽  
pp. 2650-2652 ◽  
Author(s):  
F. Gao ◽  
E. A. Talbot ◽  
C. H. Loring ◽  
J. J. Power ◽  
J. Dionne-Odom ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Antibody Test ◽  
Outbreak Investigation ◽  
Rapid Antibody
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