Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells

2019 ◽  
pp. 3-12 ◽  
Author(s):  
Sarah Hayton ◽  
Robert D. Trengove ◽  
Garth L. Maker
Keyword(s):  
Sample Preparation ◽  
Mammalian Cells ◽  
Reporting Standards ◽  
Adherent Mammalian Cells

scholarly journals Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Valeria Caprettini ◽  
Andrea Cerea ◽  
Giovanni Melle ◽  
Laura Lovato ◽  
Rosario Capozza ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Adherent Mammalian Cells

scholarly journals Profiling embryonic stem cell differentiation by MALDI TOF mass spectrometry: development of a reproducible and robust sample preparation workflow

The Analyst ◽  
2019 ◽  
Vol 144 (21) ◽  
pp. 6371-6381 ◽  
Author(s):  
Rachel E. Heap ◽  
Anna Segarra-Fas ◽  
Alasdair P. Blain ◽  
Greg M. Findlay ◽  
Matthias Trost
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mammalian Cells ◽  
Preparation Method ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Sample Preparation Method ◽  
Maldi Tof ◽  
Tof Ms

A fast and robust sample preparation method for MALDI TOF MS analysis of whole mammalian cells and phenotypic profiling of mESCs.

Fabrication of MSM-Based Biosensing Device for Assessing Dynamic Behavior of Adherent Mammalian Cells

2020 ◽  
Vol 20 (17) ◽  
pp. 9652-9659 ◽  
Author(s):  
Uvanesh Kasiviswanathan ◽  
Chandan Kumar ◽  
Suruchi Poddar ◽  
Satyabrata Jit ◽  
Sanjeev Kumar Mahto ◽  
...  
Keyword(s):  
Dynamic Behavior ◽  
Mammalian Cells ◽  
Adherent Mammalian Cells

Effects of harvesting and extraction methods on metabolite recovery from adherently growing mammalian cells

2020 ◽  
Vol 12 (19) ◽  
pp. 2491-2498
Author(s):  
Yun Luo ◽  
Ningbo Geng ◽  
Baoqin Zhang ◽  
Jiping Chen ◽  
Haijun Zhang
Keyword(s):  
Sample Preparation ◽  
Mammalian Cells ◽  
Extraction Methods ◽  
Sample Processing ◽  
Cell Harvesting ◽  
A Cell ◽  
Cell Sample ◽  
Metabolite Extraction

We compare the efficiencies of different cell harvesting methods and metabolite extraction methods in sample preparation procedures and provide a cell sample processing protocol which focuses on maximizing metabolite recovery ranging from polar to lipidic ones.

Miniaturized sample preparation on a digital microfluidics device for sensitive bottom-up microproteomics of mammalian cells using magnetic beads and mass spectrometry-compatible surfactants

Lab on a Chip ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 3490-3498 ◽  
Author(s):  
Jan Leipert ◽  
Andreas Tholey
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mammalian Cells ◽  
Magnetic Beads ◽  
Digital Microfluidics ◽  
Bottom Up ◽  
Polymer Surfactants

The combination of digital microfluidics and magnetic beads for removal of polymer surfactants enables sensitive LC-MS-based microproteomics analyses down to 100 mammalian cells.

scholarly journals Directional freezing for the cryopreservation of adherent mammalian cells on a substrate

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192265 ◽  
Author(s):  
Liat Bahari ◽  
Amir Bein ◽  
Victor Yashunsky ◽  
Ido Braslavsky
Keyword(s):  
Mammalian Cells ◽  
Directional Freezing ◽  
Adherent Mammalian Cells

A practical guide to microfluidic perfusion culture of adherent mammalian cells

Lab on a Chip ◽  
2007 ◽  
Vol 7 (6) ◽  
pp. 681 ◽  
Author(s):  
Lily Kim ◽  
Yi-Chin Toh ◽  
Joel Voldman ◽  
Hanry Yu
Keyword(s):  
Mammalian Cells ◽  
Perfusion Culture ◽  
Practical Guide ◽  
Microfluidic Perfusion ◽  
Adherent Mammalian Cells
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