Background:
The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial
agents of natural origin.
Objective:
The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of
Plumeria pudica.
Methods:
Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic
bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal
Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer
Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically.
Results:
Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl
acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl
acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl
nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane
were identified.
Conclusion:
Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.
The effect of physiological concentrations of bile acids on in vitro growth of Mycobacterium tuberculosis