In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

scholarly journals Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

2020 ◽  
Vol 151 ◽  
pp. 01029
Author(s):  
Nuzul Asmilia ◽  
Mahdi Abrar ◽  
Yudha Fahrimal ◽  
Amalia Sutriana ◽  
Yobeswi Husna
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Hexane Extract ◽  
Clear Zone ◽  
Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

scholarly journals Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246810
Author(s):  
Marija R. Mandić ◽  
Mariana M. Oalđe ◽  
Tanja M. Lunić ◽  
Aneta D. Sabovljević ◽  
Marko S. Sabovljević ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Line ◽  
Bioactive Compounds ◽  
Ethyl Acetate Extract ◽  
Chemical Characterization ◽  
No Production ◽  
Inhibition Rate ◽  
Immunomodulatory Effects ◽  
High Biological Activity

Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H. ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss extracts in vitro, which allows the design of new experiments aimed at detecting and characterizing bioactive compounds of the extracts and additionally elucidate detailed mechanisms of their effects.

Antimicrobial potentials, phytochemical screening and determination of bioactive compounds from Three Indian folk medicinal plants

2020 ◽  
Vol 16 ◽  
Author(s):  
V. Vanitha ◽  
S. Vijayakumar ◽  
S. Rajalakshmi
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Ft Ir

Aim: To investigate the antimicrobial efficacy of various solvents leaf extracts of Aegle marmelos, Plumbago zeylanica and Rhinacanthus nasutus against human pathogenic organisms such as Acinetobacter baumannii, Salmonella enteriditis, Bacillus cereus, Streptococcus pneumonia, Mycobacterium tuberculosis and Aspergillus fumigatous by agar well diffusion method. Background: Also, this is the first time report on MIC and MBC/MFC activities and identify the phytochemicals and functional groups using HPLC, GC-MS and FT-IR analysis. Objective: Minimum inhibitory concentration (MIC), minimum bactericidal (MBC/MFC) values were also carried out by the micro dilution method. Methods: Ethyl acetate extract of P. zeylanica showed better antimicrobial activity against the tested pathogens, especially for S. pneumoniae (40 mm) followed by ethyl acetate extract of R. nasutus (36 mm) against S. pneumoniae. While least inhibition was observed for aqueous extract of P.zeylanica against S. enteritis (10 mm). Results: The MIC ranged from 3.75 µg/ml to 60µg/ml and MBC/MFC 7.5 µg/ml to 60 µg/ml. Phytochemical analysis exhibited the presence of alkaloids, steroids, saponins, flavonoids, tannins, terpenes, phenolics and cardiac glycoside in all samples of selected plant extracts. Tannins, phenolics and glycoside were found only in the ethyl acetate extract of P.zeylanica. HPLC and GC-MS analysis of ethyl acetate leaf extract of P. zeylanica exposed ten major peaks and eleven compounds respectively. Chemical nature of the leaf extract was analysed by FT-IR spectrum. Conclusion: Ethyl acetate leaf extract of P. zeylanica showed better antimicrobial activity than other extracts as well as standard drugs. Therefore, this plant could be studied in detail for the identification of active biomolecules for antimicrobial agents.

Phytochemical screening, in vitro antioxidant and enzyme inhibitory proprieties and acute toxicity of extracts from the aerial parts of Ephedra nebrodensis, source of bioactive compounds

2021 ◽  
Vol 24 ◽  
Author(s):  
Meriem Hamoudi ◽  
Djouher Amroun ◽  
Sabrina Boutefnouchet ◽  
Chawki Bensouici ◽  
Samira Kaoula ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Total Phenolic ◽  
Aerial Parts

Background: Due to the strong association between the chemistry of medicinal plants and their biological properties, it is important to determine their phytochemical composition to justify experimental tests. Objective: The aim of this study was to evaluate the in vitro antioxidant and the enzyme inhibitory properties and to identify the bioactive compounds present in the extracts of Ephedra nebrodensis growing in Algeria. Methods: Total phenolic and flavonoids content in these extracts were quantified by Folin-Ciocalteu and aluminum chloride methods. The antioxidant capacity was assessed using DPPH, ABTS, β-carotene/linoleic acid, CUPRAC and FRAP assays and in vitro cholinesterase activity against acetylcholinesterase and butyrylcholinesterase were evaluated. The chemical constituents of the extracts were analyzed by high-performance liquid chromatography coupled to mass spectrometric detection and gas chromatography coupled to mass spectrometric detection. For the acute toxicity study, extracts were administered to mice at single dose of 2 g/kg and 5 g/kg by gavage. Results: Plant extracts were rich in phenolic compounds. Ethyl acetate extract presented the highest phenolic (238.44 ± 1.50 µg GAE /mg of extract) and flavonoid (21.12 ± 0.00 µg QE /mg of extract) contents. Likewise, ethyl acetate extract showed potent radical scavenging and reducing properties. Ethanol: acetone extract showed inhibitory activity against acetylcholinesterase, and was a potent inhibitor of butyrylcholinesterase. In all extracts, flavonoids were the most abundant compounds. The phytochemical investigation showed the presence of alkaloids (ephedrine and pseudo-ephedrine). In the acute toxicity, the LD50 was superior to 5 g/kg body weight. There are not alterations in the histology of the liver and kidneys. Conclusion: This study demonstrated a good antioxidant potential and anticholinesterase activity of aerial parts of E. nebrodensis.

Inhibitory effect of Torilis anthriscus on growth of microorganisms

2009 ◽  
Vol 4 (4) ◽  
pp. 493-498 ◽  
Author(s):  
Olgica Stefanovic ◽  
Dragana Stanojevic ◽  
Ljiljana Comic
Keyword(s):  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Antibacterial And Antifungal Activities

AbstractAntibacterial and antifungal activities of aqueous, ethanol and ethyl acetate extract of Torilis anthriscus (L.) Gmel. (Apiaceae) were tested in vitro against ten species of bacteria and five of fungi. Antimicrobial properties were determined by disk diffusion and broth tube dilution method. In the minimum inhibitory concentrations (MICs), the ethanol extract showed the highest activity, followed by the ethyl acetate extract and the aqueous extract against bacterial species, while the extracts were inactive against the tested fungi species. The most active extract was chosen to examine the effects of its combinations with commercial antibiotics by checkerboard method. The obtained results showed that the interactions between ethanol extract/streptomycin and ethanol extract/chloramphenicol were additive and indifferent against the tested human-pathogenic bacteria. Synergism and antagonism were not observed.

scholarly journals Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro

2018 ◽  
Vol 2 (1) ◽  
pp. 10 ◽  
Author(s):  
Mustafid Rasyiid ◽  
Rendi Mahadi ◽  
Krisnanda Surya Dharma ◽  
Lindia Anggraini ◽  
Rahma Nurdiyanti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phagocytic Activity ◽  
Ethyl Acetate Extract ◽  
Activity Index ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Immunomodulatory Activity ◽  
Leaf Extracts

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. The treated macrophage was then challenged for their phagocytic activity to latex beads. The antioxidant activity was done using 1,1-diphenil-2-picrilhydrazil (DPPH) with spectrophotometry technique. All treatments were done with three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytic activity followed with chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetat extract. The ethyl acetate extract was also the extract with the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol with similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract contained phenolics, flavonoids, tannins, and terpenoids.

scholarly journals In Vitro Assessment of Antiplasmodial and Antitrypanosomal Activities of Chloroform, Ethyl Acetate and Ethanol Leaf Extracts of Oedera genistifolia

2020 ◽  
Vol 10 (19) ◽  
pp. 6987
Author(s):  
Kunle Okaiyeto ◽  
Anthony I. Okoh
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Hela Cells ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Leaf Extracts ◽  
Resistance Evolution ◽  
Crude Extracts

The high resistance evolution of protozoans to the existing antiparasitic drugs has necessitated the quest for novel and effective drugs against plasmodium and trypanosome parasites. As a result, this study aimed to assess the antiplasmodial and antitrypanosomal potentials of chloroform, ethyl acetate and ethanol leaf extracts of Oedera genistifolia. Standard biochemical procedures were explored for the plant extraction and gas chromatography-mass spectroscopy (GCMS) was used to identify the bioactive compounds in the crude extracts. The cytotoxic effects of the crude extracts were assessed against human cervix adenocarcinoma (HeLa cells) and their antiparasitic activities were investigated against Plasmodium falciparum strain 3D7 and Trypanosoma brucei brucei. GCMS analyses of the crude extracts revealed the bioactive compounds that could be responsible for the biological activities. The extracts had no cytotoxic effect on HeLa cells and demonstrated good antiplasmodial activity (chloroform extract: IC50 = 11.6 µg∙mL−1, ethyl acetate extract: IC50 = 3.3 µg∙mL−1 and ethanol extract: IC50 = 3.7 µg∙mL−1). Likewise, they showed excellent antitrypanosomal activity with IC50 = 0.5 µg∙mL−1 for chloroform and ethyl acetate extracts and IC50 = 0.4 µg∙mL−1 for the ethanol extract. Findings from the present study indicated that O. genistifolia could be a good source of strong antiplasmodial and antitrypanosomal agents.

scholarly journals In vitro Antibacterial and Antitubercular Activities of Leaf Extracts of Senna occidentalis

2019 ◽  
pp. 1-8
Author(s):  
K. T. Olatunji ◽  
Y. Ya’aba ◽  
S. B. Mohammed ◽  
I. J. Akah ◽  
O. C. Daniel ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Fixed Dose ◽  
Dilution Method ◽  
Leaf Extracts ◽  
Control Drug ◽  
Micro Organisms

Aim: This study aimed to evaluate the antibacterial and antitubercular activities of ethylacetate and ethanol leaf extracts of Senna occidentalis. Study Design: Fresh leaves of Senna occidentalis collected from Suleja, Niger state were used for this study against some medically important micro-organisms viz; Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella paratyphi, Klebsiella pneumoniae, Mycobacterium bovis and Mycobacterium smegmatis. Place and Duration of Study: The study was conducted in Abuja, Nigeria at the Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development [NIPRD], from February 2019 to April 2019. Methodology: Senna occidentalis leaves were extracted successively with ethyl-acetate and ethanol. The obtained extracts were tested in vitro for antibacterial activity by agar well diffusion method, while anti-tubercular screening was carried out by broth micro-dilution method. A fixed-dose concentration of chloramphenicol was used as a control drug against the bacterial isolates while isoniazid was used as control drug against the mycobacterium isolates. Results: The in vitro antibacterial screening showed that the crude extracts exhibited varying activity against the different microbes with highest zone of inhibition at 12 mm, and anti-tubercular activity with MICs ranging from 97.6-390.6 μg/mL.  Among these extracts, ethyl-acetate extract showed significant antibacterial activity against most of the test micro-organisms. The most susceptible micro-organism was P. aeruginosa (12mm zone in ethyl-acetate at 80 mg/mL) followed by B. subtilis (10 mm zone in ethyl-acetate extract at 80 mg/mL) and E. coli (9 mm zone in ethyl-acetate extract at 80 mg/mL). The ethanol extract was the most effective in inhibiting the growth of M. smegmatis and M. bovis with MICs of 97.6 μg/mL and 195.3 μg/mL. Conclusion: The activities observed could be attributed to the presence of some active metabolites contained in the extracts which could be useful in drug development for therapeutic purposes.

scholarly journals Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro

2018 ◽  
Vol 3 (3) ◽  
pp. 73
Author(s):  
Rendi Mahadi ◽  
Mustafid Rasyiid ◽  
Krisnanda Surya Dharma ◽  
Lindia Anggraini ◽  
Rahma Nurdiyanti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Activity Index ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Immunomodulatory Activity ◽  
Leaf Extracts ◽  
Phagocytosis Activity

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. Macrophage cells separated form peritoneal fluid used RPMI medium. Phagocytosis activity and phagocytosis capacity of macrophages were performed in vitro using latex beads that suspended in phosphate buffered saline (PBS). The antioxidant activity was measured by spectrophotometry technique with 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution. All treatments were done three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytosis activity followed by chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetate extract. The ethyl acetate extract was also the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract has a high immunomodulatory activity and antioxidant activity which contained phenolics, flavonoids, tannins, and terpenoids.

scholarly journals Aktivitas Larvasida Ekstrak Daun Tumih (Combretocarpus rotundatus (Miq.) Danser) terhadap Larva Aedes aegypti

2019 ◽  
Vol 13 (1) ◽  
pp. 77
Author(s):  
Renhart Jemi ◽  
Royda Dara Ertini Damanik ◽  
Lies Indrayanti
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Functional Groups ◽  
Larvicidal Activity ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Leaf Extracts ◽  
Ft Ir

Penelitian ini bertujuan untuk mengukur kadar ekstrak daun tumih dan menguji aktivitas larvasidanya terhadap Aedes aegypti. Daun tumih dimaserasi dan difraksinasi dengan pelarut metanol, n-heksana, etil asetat, dan etanol. Aktivitas larvasida ekstrak diuji dengan konsentrasi 0, 5, 10, 25, 50, 75, dan 100 ppm. Hasil penelitian menunjukkan rendemen dari ekstraksi daun tumih pada berbagai larutan adalah sebagai berikut ekstrak metanol sebesar 15%, n-heksana 51%, etil asetat 35% dan etanol 85%. Hasil uji fitokimia menunjukkan bahwa ekstrak metanol daun tumih positif mengandung senyawa alkaloid, flavonoid, saponin, dan tanin. Pengujian larvasida ekstrak daun tumih menunjukkan pengaruh terhadap mortalitas larva Aedes aegypti. Aktivitas larvasida ekstrak daun tumih optimum pada ekstrak etil asetat dengan LC(50) = 24,54 ppm, ekstrak metanol LC(50) = 45,65 ppm, ekstrak etanol LC(50) = 46,77 ppm dan ekstrak n-heksana LC(50) = 48,97 ppm. Ekstrak etil asetat daun tumih merupakan ekstrak teraktif dalam aktivitas larvasidanya. Selanjutnya, analisis FT-IR menunjukkan adanya gugus fungsi C-H alkana dan C = C aromatik. Gugus fungsi tersebut diduga penyusun senyawa alkaloid, flavanoid, saponin, dan tanin. Hasil analisis LCMS mengindikasikan adanya 7 senyawa bioaktif yaitu hexadecyl-ferulate, 21-o-methyl toosendanopentaol, 23-acetate alismaketone, dehydroxy-24-acetate alisol, physanol, prosapogenin 2, dan stigmastan-3,6-dione Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegyptiAbstractThis research aimed to measure the content of Combretocarpus Rotundatus (Miq.) Danser leaf extracts and to test its larvicidal activity against Aedes aegypti. The leaves were macerated and fractionated using methanol, n-hexane, ethyl acetate, and ethanol. The extract contents from the leaves extraction were metanol extract of 15%, n-hexane extract of 51%, ethyl acetate extract of 35% and ethanol extract of 85%. The larvicidal activity of extracts was tested with concentration of 0, 5, 10, 25, 50, 75, and 100 ppm. Phytochemicals test exhibited that the methanol extract of Combretocarpus rotundatus (Miq.) Danser leaves contained alkaloids, flavonoids, saponins, and tannins. Larvicidal test conducted on the extracts exhibited an effect on the mortality levels against Aedes aegypti larvae. The larvicidal activity of leaf extracts was optimum in the ethyl acetate extract at LC(50) = 24.54 ppm, methanol extract at LC(50) = 45.65 ppm, ethanol extract at LC(50) = 46.77 ppm, and n-hexane extract at LC(50 = 48.97 ppm. It was found that the ethyl acetate extract was the most active larvicide. FT-IR analysis showed existing functional groups of C-H alkanes and C=C aromatics. Those functional groups were assumed to be flavanoid, alkaloid, saponin, and tannin constituents. Results of LC-MS analysis indicated 7 bioactive compounds i.e.hexadecyl-ferulate, 21-o-methyl toosendanopentaol, 23-acetate alismaketone, dehydroxy-24-acetate alisol, prosapogenin 2, and stigmastan-3,6- dione.

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