Evaluation of Bacterial Lipid Production: Quantitative and Qualitative Measurements: Tips and Guidelines

2019 ◽  
pp. 395-403
Author(s):  
Sima Modiri ◽  
Hossein Shahbani Zahiri ◽  
Kambiz Akbari Noghabi
Keyword(s):  
Lipid Production ◽  
Bacterial Lipid ◽  
Qualitative Measurements

scholarly journals Variation of salinity and nitrogen concentration affects the pentacyclic triterpenoid inventory of the haloalkaliphilic aerobic methanotrophic bacterium Methylotuvimicrobium alcaliphilum

Extremophiles ◽  
2021 ◽  
Author(s):  
Alexmar Cordova-Gonzalez ◽  
Daniel Birgel ◽  
Andreas Kappler ◽  
Jörn Peckmann
Keyword(s):  
Nitrogen Concentration ◽  
Lipid Production ◽  
Environmental Parameters ◽  
Full Potential ◽  
Pentacyclic Triterpenoids ◽  
Cell Functions ◽  
Pentacyclic Triterpenoid ◽  
Nitrate Concentrations ◽  
Aerobic Methanotrophs ◽  
Bacterial Lipid

AbstractThe occurrence and activity of aerobic methanotrophs are influenced by environmental conditions, including pH, temperature, salinity, methane and oxygen concentrations, and nutrient availability. Aerobic methanotrophs synthesize a variety of lipids important for cell functions. However, culture-based experiments studying the influence of environmental parameters on lipid production by aerobic methanotrophs are scarce. Such information is crucial to interpret lipid patterns of methanotrophic bacteria in the environment. In this study, the alkaliphilic strain Methylotuvimicrobium alcaliphilum was cultivated under different salinities and different nitrate concentrations to assess the effect of changing conditions on the inventory of pentacyclic triterpenoids. The results indicate that hopanoid abundance is enhanced at lower salinity and higher nitrate concentration. The production of most pentacyclic triterpenoids was favored at low salinity, especially for aminotriol. Interestingly, 3-methyl-aminotetrol and tetrahymanol were favored at higher salinity. Bacteriohopanepolyols (BHPs), particularly aminotriol and 3-methyl-aminotriol, increased considerably at higher nitrate concentrations. Four novel N-containing BHPs—aminodiol, 3-methyl-aminodiol, and isomers of aminotriol and 3-methyl-aminotriol—were identified. This study highlights the significance of environmental factors for bacterial lipid production and documents the need for cultivation studies under variable conditions to utilize the full potential of the biomarker concept.

ENVIRONMENTAL CONTROLS ON BACTERIAL LIPID PRODUCTION BY A METHANOTROPH-METHYLOTROPH CO-CULTURE

2021 ◽  
Author(s):  
N. Richter ◽  
S. Van Grinsven ◽  
L. Villanueva ◽  
E.C. Hopmans ◽  
N. Bale ◽  
...  
Keyword(s):  
Lipid Production ◽  
Environmental Controls ◽  
Bacterial Lipid

Untangling the Metabolic Reprogramming in Brain Cancer: Discovering Key Molecular Players Using Mass Spectrometry

2019 ◽  
Vol 19 (17) ◽  
pp. 1521-1534 ◽  
Author(s):  
Anatoly Sorokin ◽  
Vsevolod Shurkhay ◽  
Stanislav Pekov ◽  
Evgeny Zhvansky ◽  
Daniil Ivanov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Brain Cancer ◽  
De Novo ◽  
Aerobic Glycolysis ◽  
Lipid Production ◽  
Dietary Fatty Acids ◽  
Metabolic Reprogramming ◽  
Detection Methods ◽  
Malignant Cells ◽  
Proliferating Cells

Cells metabolism alteration is the new hallmark of cancer, as well as an important method for carcinogenesis investigation. It is well known that the malignant cells switch to aerobic glycolysis pathway occurring also in healthy proliferating cells. Recently, it was shown that in malignant cells de novo synthesis of the intracellular fatty acid replaces dietary fatty acids which change the lipid composition of cancer cells noticeably. These alterations in energy metabolism and structural lipid production explain the high proliferation rate of malignant tissues. However, metabolic reprogramming affects not only lipid metabolism but many of the metabolic pathways in the cell. 2-hydroxyglutarate was considered as cancer cell biomarker and its presence is associated with oxidative stress influencing the mitochondria functions. Among the variety of metabolite detection methods, mass spectrometry stands out as the most effective method for simultaneous identification and quantification of the metabolites. As the metabolic reprogramming is tightly connected with epigenetics and signaling modifications, the evaluation of metabolite alterations in cells is a promising approach to investigate the carcinogenesis which is necessary for improving current diagnostic capabilities and therapeutic capabilities. In this paper, we overview recent studies on metabolic alteration and oncometabolites, especially concerning brain cancer and mass spectrometry approaches which are now in use for the investigation of the metabolic pathway.

Dynamic flux balance analysis of biomass and lipid production by Antarctic thraustochytrid Oblongichytrium sp. RT2316‐13

2020 ◽  
Vol 117 (10) ◽  
pp. 3006-3017 ◽  
Author(s):  
Carolina Shene ◽  
Paris Paredes ◽  
Liset Flores ◽  
Allison Leyton ◽  
Juan A. Asenjo ◽  
...  
Keyword(s):  
Flux Balance Analysis ◽  
Lipid Production ◽  
Dynamic Flux Balance Analysis ◽  
Flux Balance ◽  
Balance Analysis

Enhancement of lipid production in Desmodesmus intermedius Z8 by ultrasonic stimulation coupled with nitrogen and phosphorus stress

2021 ◽  
pp. 108061
Author(s):  
Zhi Li ◽  
Shang-tian Yang ◽  
Zhi-you Zhou ◽  
Shuai-ying Peng ◽  
Qing-hua Zhang ◽  
...  
Keyword(s):  
Lipid Production ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Stress ◽  
Ultrasonic Stimulation

scholarly journals Human crypt intestinal epithelial cells are capable of lipid production, apolipoprotein synthesis, and lipoprotein assembly

2000 ◽  
Vol 41 (1) ◽  
pp. 12-22 ◽  
Author(s):  
Emile Levy ◽  
Jean-François Beaulieu ◽  
Edgard Delvin ◽  
Ernest Seidman ◽  
Wagner Yotov ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Lipid Production ◽  
Intestinal Epithelial ◽  
Lipoprotein Assembly

scholarly journals Insights into the physiology of Chlorella vulgaris cultivated in sweet sorghum bagasse hydrolysate for sustainable algal biomass and lipid production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Neha Arora ◽  
George P. Philippidis
Keyword(s):  
Sweet Sorghum ◽  
Lipid Production ◽  
Scale Up ◽  
Cellular Growth ◽  
Sweet Sorghum Bagasse ◽  
Triacylglycerol Synthesis ◽  
Sugar Addition ◽  
Dry Cell Weight ◽  
Sorghum Bagasse ◽  
Mixotrophic Conditions

AbstractSupplementing cultivation media with exogenous carbon sources enhances biomass and lipid production in microalgae. Utilization of renewable organic carbon from agricultural residues can potentially reduce the cost of algae cultivation, while enhancing sustainability. In the present investigation a medium was developed from sweet sorghum bagasse for cultivation of Chlorella under mixotrophic conditions. Using response surface methodology, the optimal values of critical process parameters were determined, namely inoculum cell density (O.D.750) of 0.786, SSB hydrolysate content of the medium 25% v/v, and zero medium salinity, to achieve maximum lipid productivity of 120 mg/L/d. Enhanced biomass (3.44 g/L) and lipid content (40% of dry cell weight) were observed when the alga was cultivated in SSB hydrolysate under mixotrophic conditions compared to heterotrophic and photoautotrophic conditions. A time course investigation revealed distinct physiological responses in terms of cellular growth and biochemical composition of C. vulgaris cultivated in the various trophic modes. The determined carbohydrate and lipid profiles indicate that sugar addition to the cultivation medium boosts neutral lipid synthesis compared to structural lipids, suggesting that carbon flux is channeled towards triacylglycerol synthesis in the cells. Furthermore, the fatty acid profile of lipids extracted from mixotrophically grown cultures contained more saturated and monosaturated fatty acids, which are suitable for biofuel manufacturing. Scale-up studies in a photobioreactor using SSB hydrolysate achieved a biomass concentration of 2.83 g/L consisting of 34% lipids and 26% carbohydrates. These results confirmed that SSB hydrolysate is a promising feedstock for mixotrophic cultivation of Chlorella and synthesis of algal bioproducts and biofuels.

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