Enhancement of lipid production in Desmodesmus intermedius Z8 by ultrasonic stimulation coupled with nitrogen and phosphorus stress

2021 ◽  
pp. 108061
Author(s):  
Zhi Li ◽  
Shang-tian Yang ◽  
Zhi-you Zhou ◽  
Shuai-ying Peng ◽  
Qing-hua Zhang ◽  
...  
Keyword(s):  
Lipid Production ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Stress ◽  
Ultrasonic Stimulation

Optimization of lipid accumulation in Pleurastrum insigne for biodiesel production

2021 ◽  
Vol 16 (10) ◽  
pp. 144-155
Author(s):  
Van Lal Michael Chhandama ◽  
Belur Kumudini Satyan
Keyword(s):  
Lipid Content ◽  
Lipid Production ◽  
Statistical Design ◽  
High Growth ◽  
High Growth Rate ◽  
Biodiesel Production ◽  
Nitrogen And Phosphorus ◽  
Biodiesel Standards ◽  
High Lipid ◽  
Different Sources

Microalgae emerged as a competent feedstock for biodiesel production because of high growth rate and lipid content. This work focuses on isolation of novel microalgal strain from different sources of water for the production of biodiesel. The isolated microalgae, Pleurastrum insigne possessed high lipid content (~28 % dcw), further optimized to 57.06 % dcw using a statistical design (CCD) under Response Surface Methodology. Lipid production was optimized by nutrient (nitrogen and phosphorus) and pH stress. The different type of fatty acids present in the optimized lipid was also profiled using GCMS. Biodiesel yield was found to be 82.14 % of the total lipid and the fuel properties tested have met IS, ASTM and EN biodiesel standards.

Cultivation of Chlorella sp. Using Industrial Effluents for Lipid Production

2014 ◽  
Vol 931-932 ◽  
pp. 1111-1116
Author(s):  
Tarinee Sasibunyarat ◽  
Benjamas Cheirsilp ◽  
Boonya Charnnok ◽  
Sumate Chaiprapat
Keyword(s):  
Palm Oil ◽  
Lipid Content ◽  
Mass Production ◽  
Negative Impact ◽  
Lipid Production ◽  
Industrial Effluents ◽  
High Mass ◽  
Phosphorus Concentration ◽  
Nitrogen And Phosphorus ◽  
Palm Oil Mill

This work aims to investigate microalgal growth and lipid production from Chlorella sp. on different digester effluents from seafood factory, starch factory and palm oil mill. Results under 32 cultivation days showed that the effluent from seafood factory gave the highest microalgal growth (0.9956±0.2121 g/L) followed by starch factory and palm oil mill (0.8622±0.0391 and 0.2611±0.0444 g/L, respectively). Although higher nitrogen and phosphorus in medium stimulated growth, turbidity of the palm oil mill effluent showed a negative impact. In addition, phosphorus concentration in the medium positively affected the lipid content in cells. The seafood effluent with total phosphorus of 45.24±3.80 mg/L yielded highest lipid content at 26.96±1.58% compared to starch factory (22.10±2.61). The digester effluent from seafood factory was found more suitable for Chlorella sp. cultivation due to the high mass production, oil content and lipid productivity.

Carbon, nitrogen, and phosphorus removal, and lipid production by three saline microalgae grown in synthetic wastewater irradiated with different photon fluxes

2018 ◽  
Vol 34 ◽  
pp. 97-103 ◽  
Author(s):  
Manuel Sacristán de Alva ◽  
Víctor Manuel Luna Pabello ◽  
María Teresa Orta Ledesma ◽  
Modesto Javier Cruz Gómez
Keyword(s):  
Phosphorus Removal ◽  
Lipid Production ◽  
Synthetic Wastewater ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal

Response of energy microalgae Chlamydomonas reinhardtii to nitrogen and phosphorus stress

2017 ◽  
Vol 25 (6) ◽  
pp. 5762-5770 ◽  
Author(s):  
Yizheng Wang ◽  
Jiang Yu ◽  
Ping Wang ◽  
Siwei Deng ◽  
Jiahua Chang ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Stress

Detecting Nitrogen and Phosphorus Stress in Corn Using Multi-spectral Imagery

2004 ◽  
Vol 35 (3-4) ◽  
pp. 505-516 ◽  
Author(s):  
Shannon L. Osborne ◽  
James S. Schepers ◽  
Mike R. Schlemmer
Keyword(s):  
Nitrogen And Phosphorus ◽  
Phosphorus Stress

scholarly journals Shared differentially expressed genes of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) seedlings under low nitrogen and phosphorus stress

2017 ◽  
Author(s):  
JianHui Li ◽  
GuiFang Ma ◽  
DingWei Luo ◽  
ZaiKang Tong ◽  
Jinliang Xu ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Metabolic Pathways ◽  
Chinese Fir ◽  
Cunninghamia Lanceolata ◽  
Utilization Efficiency ◽  
Differentially Expressed ◽  
Nitrogen And Phosphorus ◽  
Stress Group ◽  
Phosphorus Stress ◽  
Low Nitrogen

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is an excellent fast-growing timber species and has significant value in the forestry industry. In order to increase the nitrogen and phosphorus absorption and utilization in Chinese fir, shared differentially expressed genes under low nitrogen and phosphorus stress were screened in this study. Seedling of Chinese fir clone X6 was cultivated in aeroponic system with 3 treatments, namely, low nitrogen (LN), low phosphorus (LP) and a control check (with nitrogen and phosphorus sufficient, CK). After 4 months of treatment, the roots from the LN, LP and CK groups were collected and transcriptome sequencing was done by LC Sciences (USA) using an Illumina platform. When comparing the LN stress group with the CK group, 977 SDGEs were detected, 264 of which had KEGG annotations; 931 SDGEs were detected when comparing the LP stress group with the CK group, of which 189 had KEGG annotations; 297 SDGEs were detected in both the LN stress and LP stress groups, 78 of which had KEGG annotations representing 98 metabolic pathways. Among the 78 selected SDEGs that were differentially expressed under both LN and LP stress conditions, Twenty-one SDEGs were selected based on the metabolic pathways that nitrogen and phosphorus are involved in, these genes are PNR, PSBA, EGLC, GLC, END, BGLU, AMY, A1E, PAL, GOGAT, NIR1, NIR2, C4M, PAL, PRDX6, POX, CCR, CCoAOMT, FDH, CHS and ANR. These genes can potentially be used in breeding to improve both nitrogen and phosphorus utilization efficiency in Chinese fir.

EFFECTS OF NITROGEN AND PHOSPHORUS STRESS ON THE GROWTH OF BOTRYOCOCCUS BRAUNII

2010 ◽  
Vol 36 (3) ◽  
pp. 517-524
Author(s):  
Kai-Feng SUN ◽  
Zhang-Xi HU ◽  
Shun-Shan DUAN
Keyword(s):  
Botryococcus Braunii ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Stress

scholarly journals Porphyridium cruentum Grown in Ultra-Filtered Swine Wastewater and Its Effects on Microalgae Growth Productivity and Fatty Acid Composition

Energies ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 3194
Author(s):  
Humeyra B. Ulusoy Erol ◽  
Mariana Lara Menegazzo ◽  
Heather Sandefur ◽  
Emily Gottberg ◽  
Jessica Vaden ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Production ◽  
Biomass Productivity ◽  
Swine Wastewater ◽  
Control Medium ◽  
Nitrogen And Phosphorus ◽  
Significant Interest ◽  
Significant Uptake ◽  
Fatty Acid Compositions ◽  
Microalgae Growth

Microalgae have been extensively tested for their ability to create bio-based fuels. Microalgae have also been explored as an alternative wastewater treatment solution due to their significant uptake of nitrogen and phosphorus, as well as their ability to grow in different water types. Recently, there has been significant interest in combining these two characteristics to create economic and environmentally friendly biofuel using wastewater. This study examined the growth and lipid production of the microalgae Porphyridium (P.) cruentum grown in swine wastewater (ultra-filtered and raw) as compared with control media (L−1, modified f/2) at two different salt concentrations (seawater and saltwater). The cultivation of P. cruentum in the treated swine wastewater media (seawater = 5.18 ± 2.3 mgL−1day−1, saltwater = 3.32 ± 1.93 mgL−1day−1) resulted in a statistically similar biomass productivity compared to the control medium (seawater = 2.61 ± 2.47 mgL−1day−1, saltwater = 6.53 ± 0.81 mgL−1day−1) at the corresponding salt concentration. Furthermore, no major differences between the fatty acid compositions of microalgae in the treated swine wastewater medium and the control medium were observed. For all conditions, saturated acids were present in the highest amounts (≥67%), followed by polyunsaturated (≤22%) and finally monounsaturated (≤12%). This is the first study to find that P. cruentum could be used to remediate wastewater and then be turned into fuel by using swine wastewater with a similar productivity to the microalgae grown in control media.

scholarly journals Penapisan Mikroalga Penghasil Karotenoid serta Studi Pengaruh Stres Nitrogen dan Fosfor terhadap Produksi B-Karoten pada Mikroalga Oocystis sp.

2019 ◽  
Vol 14 (1) ◽  
pp. 9
Author(s):  
Wahida Nia Elfiza ◽  
Abdi Dharma ◽  
Nasril Nasir
Keyword(s):  
Chlorophyll A ◽  
Growth Medium ◽  
Biomass Productivity ◽  
Dry Weight ◽  
Chlorophyll B ◽  
Nitrogen And Phosphorus ◽  
Total Carotenoids ◽  
Screening Process ◽  
Phosphorus Stress ◽  
West Sumatra

Abstrakb-karoten merupakan karotenoid yang bermanfaat sebagai antioksidan. Penelitian ini bertujuan menapis mikroalga yang berpotensi  mengandung karotenoid tinggi dan mempelajari pengaruh nitrogen dan fosfor terhadap produktivitas biomassa, kandungan pigmen fotosintesis dan b-karoten, pada mikroalga yang diisolasi dari perairan Danau Atas, Sumatra Barat. Penapisan mikroalga penghasil karotenoid dilakukan  dengan memberikan  paparan UV-A 326 nm terhadap kultur campuran mikroalga. Hasil penelitian menunjukkan bahwa 5 dari 18 spesies mampu bertahan pada proses penapisan. Pengaruh 9 jenis medium pertumbuhan dengan kriteria: tanpa NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, Bold Basalt Medium (BBM) normal (kontrol), tanpa KH2PO4, 3x KH2PO4, 5x KH2PO4,dan10x KH2PO4 terhadap mikroalga terpilih (Oocystis sp.) diamati. Hasil menunjukkan kandungan b-karoten tertinggi  ditemukan pada perlakuan 5x KH2PO4  yaitu sebesar 0,22 % dari berat kering mikroalga, dengan produktivitas biomassa 0,0015 g/mL/hari, serta kandungan klorofil a, klorofil b dan karotenoid total yaitu 7,15 µg/mL, 0,81 µg/mL dan 6,67 µg/mL. Berdasarkan penelitian ini dapat disimpulkan medium pertumbuhan dengan kandungan 5x KH2PO4 merupakan medium yang cocok bagi pertumbuhan Oocystis sp. untuk mendapatkan biomassa dengan kandungan b-karoten dan karotenoid tinggi tanpa harus menurunkan produktivitas biomassanya. Screening of Carotenoid Producing Microalgae and Study of the Effect of Nitrogen and Phosphorus Stress on the Production of b-Carotene in Microalgae Oocystis sp.Abstractb-carotene is a carotenoid that is useful as an antioxidant. Present study aims to screening high microalgae which contain carotenoids from water of Danau Atas lake (West Sumatra Province), and study the effect of nitrogen and phosphorus on biomass productivity, photosynthetic and b-carotene pigment content. Screening of carotenoid-producing microalgae was carried out by exposing UV-A 326 nm to mixed microalgae cultures. The results showed that 5 of the 18 species were able to survive in the screening process. The effect of growth medium, i.e. without NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, normal Bold Basalt Medium (BBM) (control), without KH2PO4, 3x KH2PO4, 5x KH2PO4, and 10x KH2PO4 on of selected microalgae (Oocystis sp.) was carried out. The result showed that the highest content of b-carotene of Oocystis sp. was obtained with 5x KH2PO4 which was 0.22% of the dry weight of biomass. Biomass productivity was 0.0015 g/mL/day and the chlorophyll a, chlorophyll b and total carotenoids contents were 7.15 µg/mL, 0.81 µg/mL and 6.67 µg/mL, respectively. Based on this research, 5x KH2PO4 can be concluded as a suitable medium for Oocystis sp. to obtained high b -carotene and carotenoid of Oocystis sp. without  reducing biomass productivity.

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