Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy

2019 ◽  
pp. 237-244
Author(s):  
Steven Liang ◽  
James B. Mahony
Keyword(s):  
Immunofluorescent Microscopy

scholarly journals Function of integrin in duodenal mucosal uptake of iron

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 517-521 ◽  
Author(s):  
ME Conrad ◽  
JN Umbreit ◽  
RD Peterson ◽  
EG Moore ◽  
KP Harper
Keyword(s):  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Binding Protein ◽  
Iron Binding ◽  
Immunofluorescent Microscopy ◽  
Mucosal Surfaces ◽  
Iron Binding Protein ◽  
Mucosal Uptake

Abstract A mechanism for the absorption of inorganic iron in the small intestine is described in which integrins appear to play an important role in the passage of iron across microvillous membranes. Biochemical isolates from microvillous preparations of duodenum from rats dosed with radioiron showed radioactivity concentrated in integrins. The presence of integrins on mucosal surfaces of duodenal cells was confirmed by immunofluorescent microscopy using anti-integrin monoclonal antibodies. Immunoprecipitation methods were used to show that microvillous radioiron was precipitated with anti-integrin antibodies and that mobilferrin, a 56-Kd cytosol iron-binding protein, coprecipitated with integrins. We postulate from these data that the mucosal uptake of iron from the gut lumen is mediated via an integrin-mobilferrin pathway.

scholarly journals B15 Survey of Orientia tsutsugamushi-infection rate of trombiculid mites distributed in Kyoto Prefecture by immunofluorescent microscopy

1998 ◽  
Vol 49 (Supplement) ◽  
pp. 65
Author(s):  
H. Urakami ◽  
K. Okubo ◽  
M. Takahashi ◽  
H. Misumi ◽  
S. Nakajima ◽  
...  
Keyword(s):  
Infection Rate ◽  
Orientia Tsutsugamushi ◽  
Immunofluorescent Microscopy ◽  
Trombiculid Mites

An Antisera for the Fluorescent Labeling of Mouse Amelogenesis

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 1008-1009 ◽  
Author(s):  
H. Hyatt-Fischer ◽  
J. Chrispens ◽  
D. O'Keefe ◽  
H.C. Slavkin
Keyword(s):  
Extracellular Matrix ◽  
New Zealand ◽  
Tooth Development ◽  
Fluorescent Labeling ◽  
Molar Tooth ◽  
Maxillary Incisor ◽  
Enamel Matrix ◽  
Immunofluorescent Microscopy ◽  
New Zealand White Rabbits ◽  
Indirect Immunofluorescent

Embryonic mammalian enamel extracellular matrix is immunogenic. Antisera has been produced in New Zealand white rabbits using 5-day-old (postnatal) C57B1/6J mandibular and maxillary incisor and molar tooth organs as immunogens. The expression of secretory amelogenesis in mouse molar tooth organs was studied from the "cap stage" (circa 17-day fetus) to the fifth day of postnatal odontogenesis using indirect immunofluorescent microscopy. The specificity of the antisera for enamel matrix secretion was unequivocal. Secretory amelogenesis was observed in molar tooth organs as early as day-2 postnatal age. These reagents and methods provide a significant strategy in studies of epithelial-mesenchymal interactions during tooth development.

Immunofluorescent Evidence for the Similarity of Amelogenins in Calf, Mouse and Pig Teeth

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 992-993 ◽  
Author(s):  
R. Herold ◽  
P. Christner ◽  
H. Graver ◽  
J. Rosenbloom
Keyword(s):  
Specific Antiserum ◽  
Enamel Matrix ◽  
Immunofluorescent Microscopy ◽  
Thin Line ◽  
Fetal Bovine ◽  
Tooth Buds ◽  
Bovine Enamel

Antiserum was prepared to fetal bovine enamel matrix and was used to localize the amelogenins in developing bovine molars by immunofluorescent microscopy. Amelogenins could be identified to preameloblasts, secretory ameloblasts, stratum intermedium cells, and the newly deposited enamel matrix. Mature enamel matrix did not fluoresce except in a thin line along the DEJ and adjacent to the ameloblasts. Immature enamel matrix of murine and porcine teeth fluoresced when treated with antiserum to bovine enamel matrix. No other portions of tooth buds or other tissues reacted with the specific antiserum.

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