An Antisera for the Fluorescent Labeling of Mouse Amelogenesis

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 1008-1009 ◽  
Author(s):  
H. Hyatt-Fischer ◽  
J. Chrispens ◽  
D. O'Keefe ◽  
H.C. Slavkin
Keyword(s):  
Extracellular Matrix ◽  
New Zealand ◽  
Tooth Development ◽  
Fluorescent Labeling ◽  
Molar Tooth ◽  
Maxillary Incisor ◽  
Enamel Matrix ◽  
Immunofluorescent Microscopy ◽  
New Zealand White Rabbits ◽  
Indirect Immunofluorescent

Embryonic mammalian enamel extracellular matrix is immunogenic. Antisera has been produced in New Zealand white rabbits using 5-day-old (postnatal) C57B1/6J mandibular and maxillary incisor and molar tooth organs as immunogens. The expression of secretory amelogenesis in mouse molar tooth organs was studied from the "cap stage" (circa 17-day fetus) to the fifth day of postnatal odontogenesis using indirect immunofluorescent microscopy. The specificity of the antisera for enamel matrix secretion was unequivocal. Secretory amelogenesis was observed in molar tooth organs as early as day-2 postnatal age. These reagents and methods provide a significant strategy in studies of epithelial-mesenchymal interactions during tooth development.

"Proenamel → Enamel → Polypeptides": A Concept

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 988-990 ◽  
Author(s):  
J. Chrispens ◽  
B. Weliky ◽  
P. Bringas ◽  
H. Slavkin
Keyword(s):  
New Zealand ◽  
Developmental Stage ◽  
Protein Gene ◽  
Protein S ◽  
Molar Tooth ◽  
Matrix Proteins ◽  
Gene Products ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Enamel Protein

Our laboratory is interested in determining the number of enamel protein gene products which characterize the ameloblast phenotype, and to what extent these proteins are degraded as a function of enamel maturation. Many investigators have reported a large number of heterogeneous proteins within the enamel matrix (Eggert, Allen and Burgess, 1973; Weidmann and Eyre, 1971). One explanation for this apparent heterogeneity may be the uncertain age of the analyzed enamel matrix; specimens may have undergone degradative processes associated with enamel maturation. To overcome this difficulty, our laboratory selected 26-day embryonic New Zealand White (NZW) rabbit incisor and molar tooth organs to isolate enamel protein(s). At this developmental stage it is possible to identify newly-secreted enamel matrix proteins.

Ultrastructure of Ligament Extracellular Matrix After Chondroitinase ABC Digestion

1988 ◽  
Vol 46 ◽  
pp. 250-251
Author(s):  
D. F. Bray ◽  
C. Frank
Keyword(s):  
Extracellular Matrix ◽  
New Zealand ◽  
Osmium Tetroxide ◽  
Caproic Acid ◽  
Ground Substance ◽  
Collateral Ligaments ◽  
Ethanol Dehydration ◽  
Chondroitinase Abc ◽  
Major Drawback ◽  
New Zealand White Rabbits

The cationic stain ruthenium hexammine trichloride (RHT) has been utilized as a connective tissue stain primarily due to its ability to stabilize and stain anionically-charged glycosaminoglycans (GAGs). A major drawback of this procedure however is that the increased preservation and visibility of the GAGs obscures other RHT-stained constituents of the “ground substance”. In order to more readily visualize these non-GAG ground substance components ligament tissue was pre-treated with chondroitinase ABC, an enzyme that removes most, if not all of the GAGs present.Segments of the midsubstance of medial collateral ligaments (MCL) of mature New Zealand white rabbits were excised and incubated 1 hat 37°C in chondroitinase ABC (1 U/ml in 0.25M Tris, 0.18M NaCl, 5mM benzamidine HC1, 0. 1. 6-amino-n-caproic acid, pH 8.0) followed by fixation in Na-cacodylate (0.1M, pH 7.4) buffered glutaraldehyde (2.5%) containing RHT (lmg/ml) for 2.5 h. Post-fixation for 1 h in cacodylate-buffered osmium tetroxide (1%) plus RHT (lmg/ml) preceded ethanol dehydration and embedding in Spurr's resin.

Demonstration of an Alloimmune Response to Embryonic Enamel Matrix Proteins

1975 ◽  
Vol 54 (3_suppl) ◽  
pp. 72-77 ◽  
Author(s):  
Steven E. Schonfeld
Keyword(s):  
Young Adult ◽  
Specific Binding ◽  
Matrix Proteins ◽  
Antigenic Determinants ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Incisor Tooth ◽  
Immunofluorescent Microscopy ◽  
Enamel Proteins ◽  
Indirect Immunofluorescent

Young adult rabbits were immunized with enamel matrix proteins from embryonic tooth organs of the same strain of rabbit. These proteins elicited an alloimmune response as demonstrated by the specific binding of antiserum to enamel matrix which was visualized by indirect immunofluorescent microscopy. The labial and lingual surfaces of embryonic incisor tooth organs were found to share common antigenic determinants. The observations suggest that enamel proteins could possibly be autoantigens.

A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

1975 ◽  
Vol 33 ◽  
pp. 460-461
Author(s):  
Sidney D. Kobernick ◽  
Edna A. Elfont ◽  
Neddra L. Brooks
Keyword(s):  
Lipid Metabolism ◽  
New Zealand ◽  
Aortic Wall ◽  
Rabbit Aorta ◽  
Plaque Formation ◽  
Metabolic Changes ◽  
Atherosclerotic Plaques ◽  
Cytochemical Study ◽  
Cellular Alteration ◽  
New Zealand White Rabbits

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

scholarly journals An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 880
Author(s):  
Tuanyuan Shi ◽  
Xinlei Yan ◽  
Hongchao Sun ◽  
Yuan Fu ◽  
Lili Hao ◽  
...  
Keyword(s):  
New Zealand ◽  
Body Weight Gain ◽  
Animal Experiments ◽  
Zealand White Rabbit ◽  
Opportunistic Pathogen ◽  
Large Numbers ◽  
New Zealand White Rabbits ◽  
Positive Rate ◽  
Relationship Of ◽  
The Relationship

Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit diarrhoea needs to be clearly identified. In this study, a C. guttulatus Zhejiang strain was isolated from a New Zealand White rabbit with severe diarrhoea and then inoculated into SPF New Zealand white rabbits alone or co-inoculated with Eimeriaintestinalis, another kind of pathogen in rabbits. Our results showed that the optimal culture medium pH and temperature for this yeast were pH 4.5 and 40–42 °C, respectively. The sequence lengths of the 18S and 26S ribosomal DNA fragments were 1559 bp and 632 bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from dogs and 100% homology with the 26S ribosomal sequence of DPA-CGR1 and CGDPA-GP1 isolates from rabbits and guinea pigs, respectively. In animal experiments, the C. guttulatus Zhejiang strain was not pathogenic to healthy rabbits, even when 1 × 108 vegetative cells were used per rabbit. Surprisingly, rabbits inoculated with yeast showed a slightly better body weight gain and higher food intake. However, SPF rabbits co-inoculated with C. guttulatus and E. intestinalis developed more severe coccidiosis than rabbits inoculated with C. guttulatus or E. intestinalis alone. In addition, we surveyed the prevalence of C. guttulatus in rabbits and found that the positive rate was 83% in Zhejiang Province. In summary, the results indicated that C. guttulatus alone is not pathogenic to healthy rabbits, although might be an opportunistic pathogen when the digestive tract is damaged by other pathogens, such as coccidia.

scholarly journals Aspirin treatment prevents inflammation in experimental bifurcation aneurysms in New Zealand White rabbits

2021 ◽  
pp. neurintsurg-2020-017261
Author(s):  
Stefan Wanderer ◽  
Basil Erwin Grüter ◽  
Fabio Strange ◽  
Gwendoline Boillat ◽  
Sivani Sivanrupan ◽  
...  
Keyword(s):  
New Zealand ◽  
Clinical Situation ◽  
Fluorescence Angiography ◽  
Preventive Effect ◽  
Aneurysm Formation ◽  
Aneurysm Wall ◽  
New Zealand White Rabbits ◽  
Aneurysm Thrombosis ◽  
Wall Groups

BackgroundAneurysm wall degeneration is linked to growth and rupture. To address the effect of aspirin (ASA) on aneurysm formation under various wall conditions, this issue was analyzed in a novel rabbit bifurcation model.MethodsBifurcation aneurysms created in 45 New Zealand White rabbits were randomized to vital (n=15), decellularized (n=13), or elastase-degraded (n=17) wall groups; each group was assigned to a study arm with or without ASA. At follow-up 28 days later, aneurysms were evaluated for patency, growth, and wall inflammation at macroscopic and histological levels.Results36 rabbits survived to follow-up at the end of the trial. None of the aneurysms had ruptured. Patency was visualized in all aneurysms by intraoperative fluorescence angiography and confirmed in 33 (92%) of 36 aneurysms by MRI/MRA. Aneurysm size was significantly increased in the vital (without ASA) and elastase-degraded (with and without ASA) groups. Aneurysm thrombosis was considered complete in three (50%) of six decellularized aneurysms without ASA by MRI/MRA. Locoregional inflammation of the aneurysm complex was significantly reduced in histological analysis among all groups treated with ASA.ConclusionASA intake prevented inflammation of both the periadventitial tissue and aneurysm wall, irrespective of initial wall condition. Although ASA prevented significant growth in aneurysms with vital walls, this preventive effect did not have an important role in elastase-degraded pouches. In possible translation to the clinical situation, ASA might exert a potential preventive effect during early phases of aneurysm formation in patients with healthy vessels but not in those with highly degenerative aneurysm walls.

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