Large-Scale Purification of Latex Bead Phagosomes from Mouse Macrophage Cell Lines and Subsequent Preparation for High-Throughput Quantitative Proteomics

2008 ◽  
pp. 339-351 ◽  
Author(s):  
Adam Rupper ◽  
James Cardelli
Keyword(s):  
Cell Lines ◽  
High Throughput ◽  
Quantitative Proteomics ◽  
Large Scale ◽  
Latex Bead ◽  
Mouse Macrophage ◽  
Macrophage Cell

scholarly journals Cytotoxic Profiling of Annotated and Diverse Chemical Libraries Using Quantitative High-Throughput Screening

2019 ◽  
Vol 25 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Olivia W. Lee ◽  
Shelley Austin ◽  
Madison Gamma ◽  
Dorian M. Cheff ◽  
Tobie D. Lee ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Drug Targets ◽  
Large Scale ◽  
Early Stage ◽  
Cancer Cell Line ◽  
Hek 293 ◽  
Cytotoxic Compounds

Cell-based phenotypic screening is a commonly used approach to discover biological pathways, novel drug targets, chemical probes, and high-quality hit-to-lead molecules. Many hits identified from high-throughput screening campaigns are ruled out through a series of follow-up potency, selectivity/specificity, and cytotoxicity assays. Prioritization of molecules with little or no cytotoxicity for downstream evaluation can influence the future direction of projects, so cytotoxicity profiling of screening libraries at an early stage is essential for increasing the likelihood of candidate success. In this study, we assessed the cell-based cytotoxicity of nearly 10,000 compounds in the National Institutes of Health, National Center for Advancing Translational Sciences annotated libraries and more than 100,000 compounds in a diversity library against four normal cell lines (HEK 293, NIH 3T3, CRL-7250, and HaCat) and one cancer cell line (KB 3-1, a HeLa subline). This large-scale library profiling was analyzed for overall screening outcomes, hit rates, pan-activity, and selectivity. For the annotated library, we also examined the primary targets and mechanistic pathways regularly associated with cell death. To our knowledge, this is the first study to use high-throughput screening to profile a large screening collection (>100,000 compounds) for cytotoxicity in both normal and cancer cell lines. The results generated here constitute a valuable resource for the scientific community and provide insight into the extent of cytotoxic compounds in screening libraries, allowing for the identification and avoidance of compounds with cytotoxicity during high-throughput screening campaigns.

scholarly journals Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)

2015 ◽  
Vol 6 (8) ◽  
pp. 2834-2844 ◽  
Author(s):  
Papawee Suabjakyong ◽  
Kazuhiro Nishimura ◽  
Toshihiko Toida ◽  
Leo J. L. D. Van Griensven
Keyword(s):  
Cell Lines ◽  
Structural Characterization ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Phellinus Linteus ◽  
Immunomodulatory Effects ◽  
Macrophage Cell ◽  
Phellinus Igniarius ◽  
Cytokine Balance ◽  
Asian Medicine

Phellinus linteus and igniarius (L.) Quel. have been used in traditional Asian medicine for over two centuries against a variety of diseases.

scholarly journals Lipid IVa incompletely activates MyD88-independent Toll-like receptor 4 signaling in mouse macrophage cell lines

2013 ◽  
Vol 67 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Norihiko Ogura ◽  
Masashi Muroi ◽  
Yuka Sugiura ◽  
Ken-ichi Tanamoto
Keyword(s):  
Cell Lines ◽  
Mouse Macrophage ◽  
Toll Like Receptor ◽  
Macrophage Cell ◽  
Toll Like Receptor 4

scholarly journals Cytotoxic Profiling of Annotated and Diverse Chemical Libraries Using Quantitative High-Throughput Screening

2018 ◽  
Author(s):  
Olivia W. Lee ◽  
Shelley Austin ◽  
Madison Gamma ◽  
Dorian M. Cheff ◽  
Tobie D. Lee ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Drug Targets ◽  
Large Scale ◽  
Early Stage ◽  
Cancer Cell Line ◽  
Hek 293 ◽  
Cytotoxic Compounds

AbstractCell-based phenotypic screening is a commonly used approach to discover biological pathways, novel drug targets, chemical probes and high-quality hit-to-lead molecules. Many hits identified from high-throughput screening campaigns are ruled out through a series of follow-up potency, selectivity/specificity, and cytotoxicity assays. Prioritization of molecules with little or no cytotoxicity for downstream evaluation can influence the future direction of projects, so cytotoxicity profiling of screening libraries at an early stage is essential for increasing the likelihood of candidate success. In this study, we assessed cell-based cytotoxicity of nearly 10,000 compounds in NCATS annotated libraries, and over 100,000 compounds in a diversity library, against four ‘normal’ cell lines (HEK 293, NIH 3T3, CRL-7250 and HaCat) and one cancer cell line (KB 3-1, a HeLa subline). This large-scale library profiling was analyzed for overall screening outcomes, hit rates, pan-activity and selectivity. For the annotated library, we also examined the primary targets and mechanistic pathways regularly associated with cell death. To our knowledge, this is the first study to use high-throughput screening to profile a large screening collection (>100,000 compounds) for cytotoxicity in both normal and cancer cell lines. The results generated here constitutes a valuable resource for the scientific community and provides insight on the extent of cytotoxic compounds in screening libraries, identifying and avoiding compounds with cytotoxicity during high-throughput screening campaigns.

scholarly journals Large-Scale Transcriptional Profiling of Molecular Perturbations Reveals Cell Type Specific Responses and Implications for Environmental Screening

2020 ◽  
Author(s):  
Kun Zhang ◽  
Yanbin Zhao
Keyword(s):  
Cell Lines ◽  
High Throughput ◽  
High Throughput Screening ◽  
Large Scale ◽  
Transcriptional Profiling ◽  
High Sensitivity ◽  
Environmental Chemicals ◽  
Cell Type ◽  
Cell Type Specific ◽  
Molecule Interactions

AbstractCell-based assays represent nearly half of all high-throughput screens currently conducted for risk assessment of environmental chemicals. However, the sensitivity and heterogeneity among cell lines has long been concerned but explored only in a limited manner. Here, we address this question by conducting a large scale transcriptomic analysis of the responses of discrete cell lines to specific small molecules. Our results illustrate heterogeneity of the extent and timing of responses among cell lines. Interestingly, high sensitivity and/or heterogeneity was found to be cell type-specific or universal depending on the different mechanism of actions of the compounds. Our data provide a novel insight into the understanding of cell-small molecule interactions and have substantial implications for the design, execution and interpretation of high-throughput screening assays.

Mouse monoclonal IgA binds to the galectin-3/Mac-2 lectin from mouse macrophage cell lines

2004 ◽  
Vol 93 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Rajko Reljic ◽  
Carol Crawford ◽  
Stephen Challacombe ◽  
Juraj Ivanyi
Keyword(s):  
Cell Lines ◽  
Mouse Macrophage ◽  
Macrophage Cell ◽  
Galectin 3
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