Blood Injection Subarachnoid Hemorrhage Rat Model

2009 ◽  
pp. 271-278 ◽  
Author(s):  
Hartmut Vatter ◽  
Erdem Gueresir ◽  
Volker Seifert
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Rat Model ◽  
Blood Injection

scholarly journals Effect of Vasospasm on Heme Oxygenases in a Rat Model of Subarachnoid Hemorrhage

Stroke ◽  
1998 ◽  
Vol 29 (3) ◽  
pp. 683-689 ◽  
Author(s):  
Minoru Kuroki ◽  
Kenji Kanamaru ◽  
Hidenori Suzuki ◽  
Shiro Waga ◽  
Reiji Semba
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Rat Model ◽  
Heme Oxygenases

The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model

2021 ◽  
Vol 1765 ◽  
pp. 147508
Author(s):  
İlker Güleç ◽  
Aslıhan Şengelen ◽  
Feyza Karagöz-Güzey ◽  
Evren Önay-Uçar ◽  
Burak Eren ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Signaling Pathway ◽  
Rat Model

Treatment of early brain injury after subarachnoid hemorrhage in the rat model by inhibiting p53-induced ferroptosis

2021 ◽  
pp. 136134
Author(s):  
Hong Kuang ◽  
Tianhong Wang ◽  
Lei Liu ◽  
Chunhai Tang ◽  
Tao Li ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Brain Injury ◽  
Rat Model ◽  
Early Brain Injury

Blood Injection Subarachnoid Hemorrhage Mouse Model

2009 ◽  
pp. 287-292
Author(s):  
Tarkan Calisaneller ◽  
Chih-Lung Lin ◽  
Naoya Ukita ◽  
Ryo Yoshimura ◽  
Yi Wang ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Mouse Model ◽  
Blood Injection

Abstract T P242: Cognitive Dysfunction in a Pre-chiasmatic Cistern Blood Injection Model of Aneurysmal Subarachnoid Hemorrhage In Mice

Stroke ◽  
2015 ◽  
Vol 46 (suppl_1) ◽  
Author(s):  
Fumiaki Oka ◽  
Yahya B Atalay ◽  
Tao Qin ◽  
Cenk Ayata
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Cognitive Dysfunction ◽  
Aneurysmal Subarachnoid Hemorrhage ◽  
Laser Speckle ◽  
Cisterna Magna ◽  
Arterial Blood ◽  
Y Maze ◽  
Injection Model ◽  
Blood Injection ◽  
Underlying Mechanisms

Background: Majority of aneurysmal subarachnoid hemorrhage (SAH) survivors develop cognitive dysfunction. To better understand the underlying mechanisms and develop treatments, predictive animal models are required. We carried out a detailed physiological and cognitive characterization of pre-chiasmatic cistern (PC) and cisterna magna (CM) SAH models in mice. Methods: SAH was induced by arterial blood injection into the PC (40 μl) or CM (60 μl) in C57BL6/J mice (male, 25g). Controls received normal saline. Cerebral blood flow (CBF) was imaged using laser speckle flowmetry during and for 60 min after SAH. Intracranial pressure (ICP) and blood pressure (BP) were monitored to calculate cerebral perfusion pressure (CPP). Neurological and cognitive function was assessed 3 weeks after the injection, using pole, novel object recognition, Y maze and Morris water maze tests. Results: Mortality was 10% after PC and 4% after CM SAH. In both groups, CPP decreased from about 65 mmHg to less than 10 mmHg immediately after the injection, and recovered to 40 mmHg within 10 min after PC (n=8) and 7 min after CM (n=8) SAH (Fig A). In both groups, CBF was severely reduced to ~20% of baseline in both hemispheres immediately after SAH. CBF recovered to >40% within 5 min after PC and 2 min after CM SAH (Fig A). In saline controls (n=5 in PC and CM each), CPP and CBF changes were much milder and shorter-lasting. Compared with controls (n=12), PC SAH mice (n=12) performed significantly worse in a subset of sensorimotor and cognitive tests for up to 3 weeks (Fig B). CM SAH did not significantly impact neurological function. Conclusions: Pre-chiasmatic cistern but not cisterna magna SAH model reproduces cognitive dysfunction observed in patients with low mortality and high reproducibility in mice.

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

10.3791/62567 ◽  
2021 ◽  
Author(s):  
Jesper Peter Bömers ◽  
Sara Ellinor Johansson ◽  
Lars Edvinsson ◽  
Tiit Illimar Mathiesen ◽  
Kristian Agmund Haanes
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Rat Model ◽  
Single Injection ◽  
Autologous Blood ◽  
Experimental Subarachnoid Hemorrhage

Involvement of neutrophils in machineries underlying the rupture of intracranial aneurysms

2019 ◽  
Author(s):  
Mika Kushamae ◽  
Haruka Miyata ◽  
Manabu Shirai ◽  
Kampei Shimizu ◽  
Mieko Oka ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Rat Model ◽  
Immunohistochemical Study ◽  
Therapeutic Modality ◽  
Major Type ◽  
Collagenolytic Activity ◽  
Inflammatory Stimuli ◽  
A Site ◽  
Gene Expression Profile Data

Abstract Background: Subarachnoid hemorrhage due to rupture of an intracranial aneurysm (IA) has quite a poor prognosis once after the onset despite of the modern technical advancement. The development of a novel therapeutic modality to prevent rupture or a diagnostic method to stratify dangerous lesions from many stable ones is thus mandatory for social health. To this end, mechanisms underlying rupture of lesions should be clarified. Methods: We and others have developed the rat model in which induced IAs spontaneously rupture resulting in subarachnoid hemorrhage. To clarify molecular cascades regulating rupture, we obtained gene expression profile data from rupture-prone lesions and revealed the enrichment of neutrophil-related terms in rupture-prone lesions by Gene Ontology analysis. Next, to validate a role of neutrophils in rupture of lesions, G-CSF was administered to a rat model. Results: As a result, G-CSF treatment not only increased number of neutrophils infiltrating in lesions but also significantly facilitated rupture of the lesions without increase the incidence. To clarify mechanisms how neutrophils facilitate rupture of IAs, we used HL-60 cell line and found that inflammatory stimuli enhanced the collagenolytic activity of MMP9. Immunohistochemical study using IA lesions from a rat model identified neutrophils as a major type of cells producing MMP9 around a site of rupture and consistently the collagenolytic activity of MMP9 was detected in ruptured lesions. Conclusions: These results combined together suggest the crucial role of neutrophil to rupture of IAs and also propose the potential of this type of cells as a candidate of therapeutic or diagnostic targets.

Tetramethylpyrazine Protects Against Early Brain Injury and Inhibits the PERK/Akt Pathway in a Rat Model of Subarachnoid Hemorrhage

2018 ◽  
Vol 43 (8) ◽  
pp. 1650-1659 ◽  
Author(s):  
Zhengkai Shao ◽  
Pei Wu ◽  
Xuefeng Wang ◽  
Meishan Jin ◽  
Shuang Liu ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Brain Injury ◽  
Rat Model ◽  
Early Brain Injury ◽  
Akt Pathway

PARP inhibition attenuates early brain injury through NF-κB/MMP-9 pathway in a rat model of subarachnoid hemorrhage

2016 ◽  
Vol 1644 ◽  
pp. 32-38 ◽  
Author(s):  
Ting Chen ◽  
Wei Wang ◽  
Jian-Ru Li ◽  
Hang-Zhe Xu ◽  
Yu-Cong Peng ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Brain Injury ◽  
Rat Model ◽  
Early Brain Injury ◽  
Parp Inhibition

scholarly journals The Effect of Blood Glutamate Scavengers Oxaloacetate and Pyruvate on Neurological Outcome in a Rat Model of Subarachnoid Hemorrhage

2012 ◽  
Vol 9 (3) ◽  
pp. 649-657 ◽  
Author(s):  
Matthew Boyko ◽  
Israel Melamed ◽  
Benjamin Fredrick Gruenbaum ◽  
Shaun Evan Gruenbaum ◽  
Sharon Ohayon ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Rat Model ◽  
Neurological Outcome
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