scholarly journals Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques

2010 ◽  
pp. 149-161 ◽  
Author(s):  
Rossana C.N. Melo ◽  
Heloisa D’Ávila ◽  
Patricia T. Bozza ◽  
Peter F. Weller
Keyword(s):  
Light Microscopy ◽  
Lipid Bodies ◽  
Microscopy Techniques

2013 Microscopy Today Innovation Awards

2013 ◽  
Vol 21 (5) ◽  
pp. 40-45
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Scanning Probe Microscopy ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Analysis Methods ◽  
Microscopy Analysis ◽  
Ion Microscopy ◽  
Microscopy Techniques

Microscopy Today congratulates the fourth annual group of Innovation Award winners. The ten innovations described below move several microscopy techniques forward: light microscopy, scanning probe microscopy, electron microscopy, ion microscopy, and hybrid microscopy-analysis methods. These innovations will make imaging and analysis more powerful, more flexible, more productive, and easier to accomplish.

Thin-Section Preparation and Transmitted-Light Microscopy

2010 ◽  
pp. 115-135
Keyword(s):  
Light Microscopy ◽  
Polymeric Composites ◽  
Glass Slide ◽  
Transmitted Light ◽  
Fiber Reinforced ◽  
Transmitted Light Microscopy ◽  
Ultrathin Sections ◽  
Microscopy Techniques ◽  
Surface Mounting ◽  
Selection Of

Abstract Transmitted-light methods reveal more details of the morphology of fiber-reinforced polymeric composites than are observable using any other available microscopy techniques. This chapter describes the various aspects relating to the selection and preparation of ultrathin-section specimens of fiber-reinforced polymeric composites for examination by transmitted-light microscopy techniques. The preparation steps covered are a selection of the rough section, preparation of the rough section for preliminary mounting, grinding and polishing the primary-mount first surface, mounting the first surface on a glass slide, and preparing the second surface (top surface). The optimization of microscope conditions and analysis of specimens by microscopy techniques are also covered. In addition, examples of composite ultrathin sections that are analyzed using transmitted-light microscopy contrast methods are shown throughout.

Electron Microscopy Applied to Fine-Needle Aspiration. A Report of Six Cases from Various Sites

1983 ◽  
Vol 69 (5) ◽  
pp. 423-435 ◽  
Author(s):  
Saverio Cinti ◽  
Maurizio Ferretti ◽  
Silvana Amati ◽  
Giancarlo Balercia ◽  
Adalberto Vecchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Ultrastructural Analysis ◽  
Tissue Structure ◽  
Fine Needle ◽  
Neoplastic Lesions ◽  
Microscopy Techniques

The authors report the results obtained from the application of electron microscopy techniques to the cytology of fine-needle-aspirated samples of neoplastic lesions from various body sites. These results show that the tissue structure, which is usually lost during the squashing necessary for light microscopy cytology, is preserved when the samples are processed for ultrastructural analysis. Electron microscopy also allows a highly detailed study of the cell's inner structures. Thus, when this technique is applied, fine needle-aspirated samples can be regarded as actual microbiopsies. However, because of the high cost of ultrastructural techniques, we suggest that actual analysis be performed only in selected cases, whereas fixation and inclusion for electron microscopy could be done routinely.

From Dynamics to Details: Live-Cell Light Microscopy and Highresolution (25 nm) Soft X-Ray Microscopy

2000 ◽  
Vol 6 (S2) ◽  
pp. 84-85
Author(s):  
C. A. Larabell ◽  
D. Yager ◽  
W. Meyer-Ilse ◽  
B. A. Rowning
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Cell Function ◽  
Living Cells ◽  
Live Cell ◽  
Specimen Preparation ◽  
Cellular Organization ◽  
X Ray ◽  
Structural Details ◽  
Microscopy Techniques

Imaging cells using a variety of microscopy techniques has generated information about the organization of cells and subcellular structures that is the foundation for understanding cell function. Invaluable information about the dynamics of cells has been obtained from a variety of light microscopy techniques, and the exquisite structural details of cellular organization have been obtained from electron microscopy. Each of these approaches, however, has its limitations. The resolution provided by light microscopy is limited by the wavelength of light, whereas the specimen preparation required for examining cells using electron microscopy is extremely tedious and time consuming. We show that soft x-ray microscopy can bridge the gap between these two forms of microscopy by providing a method for examining whole, hydrated cells up to 10 um thick at 25 nm resolution. Examination of rapidly frozen cells provides information that closely approximates that seen in living cells.

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