6 Light microscopy techniques for bacterial cell biology

2002 ◽  
pp. 115-132 ◽  
Author(s):  
Petra Anne Levin
Keyword(s):  
Light Microscopy ◽  
Bacterial Cell ◽  
Cell Biology ◽  
Microscopy Techniques

scholarly journals LS1C2 Introduction to multi-mode light microscopy and its application to cell biology

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S223
Author(s):  
M. Sokabe ◽  
H. Tatsumi
Keyword(s):  
Light Microscopy ◽  
Cell Biology ◽  
Multi Mode

2013 Microscopy Today Innovation Awards

2013 ◽  
Vol 21 (5) ◽  
pp. 40-45
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Scanning Probe Microscopy ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Analysis Methods ◽  
Microscopy Analysis ◽  
Ion Microscopy ◽  
Microscopy Techniques

Microscopy Today congratulates the fourth annual group of Innovation Award winners. The ten innovations described below move several microscopy techniques forward: light microscopy, scanning probe microscopy, electron microscopy, ion microscopy, and hybrid microscopy-analysis methods. These innovations will make imaging and analysis more powerful, more flexible, more productive, and easier to accomplish.

Thin-Section Preparation and Transmitted-Light Microscopy

2010 ◽  
pp. 115-135
Keyword(s):  
Light Microscopy ◽  
Polymeric Composites ◽  
Glass Slide ◽  
Transmitted Light ◽  
Fiber Reinforced ◽  
Transmitted Light Microscopy ◽  
Ultrathin Sections ◽  
Microscopy Techniques ◽  
Surface Mounting ◽  
Selection Of

Abstract Transmitted-light methods reveal more details of the morphology of fiber-reinforced polymeric composites than are observable using any other available microscopy techniques. This chapter describes the various aspects relating to the selection and preparation of ultrathin-section specimens of fiber-reinforced polymeric composites for examination by transmitted-light microscopy techniques. The preparation steps covered are a selection of the rough section, preparation of the rough section for preliminary mounting, grinding and polishing the primary-mount first surface, mounting the first surface on a glass slide, and preparing the second surface (top surface). The optimization of microscope conditions and analysis of specimens by microscopy techniques are also covered. In addition, examples of composite ultrathin sections that are analyzed using transmitted-light microscopy contrast methods are shown throughout.

Electron Microscopy Applied to Fine-Needle Aspiration. A Report of Six Cases from Various Sites

1983 ◽  
Vol 69 (5) ◽  
pp. 423-435 ◽  
Author(s):  
Saverio Cinti ◽  
Maurizio Ferretti ◽  
Silvana Amati ◽  
Giancarlo Balercia ◽  
Adalberto Vecchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Ultrastructural Analysis ◽  
Tissue Structure ◽  
Fine Needle ◽  
Neoplastic Lesions ◽  
Microscopy Techniques

The authors report the results obtained from the application of electron microscopy techniques to the cytology of fine-needle-aspirated samples of neoplastic lesions from various body sites. These results show that the tissue structure, which is usually lost during the squashing necessary for light microscopy cytology, is preserved when the samples are processed for ultrastructural analysis. Electron microscopy also allows a highly detailed study of the cell's inner structures. Thus, when this technique is applied, fine needle-aspirated samples can be regarded as actual microbiopsies. However, because of the high cost of ultrastructural techniques, we suggest that actual analysis be performed only in selected cases, whereas fixation and inclusion for electron microscopy could be done routinely.

scholarly journals A microscopic view of the cell

2016 ◽  
Vol 27 (21) ◽  
pp. 3183-3184
Author(s):  
Bo Huang
Keyword(s):  
Quantitative Analysis ◽  
Light Microscopy ◽  
Cell Biology ◽  
Integrative Approach ◽  
Biological Knowledge ◽  
Critical Links ◽  
Indispensable Tool ◽  
Biology Research

Light microscopy has long been an indispensable tool for cell biology research. From biological problems to biological knowledge, there are two more critical links in the light microscopy approach: labeling and quantitative analysis. Therefore, an integrative approach is desirable in order to deal with practical challenges in biological light microscopy.

scholarly journals Genetically encoded fluorescent tags

2017 ◽  
Vol 28 (7) ◽  
pp. 848-857 ◽  
Author(s):  
Kurt Thorn
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Light Microscopy ◽  
Cell Biology ◽  
Fluorescent Proteins ◽  
Protein Sequences ◽  
Biological Properties ◽  
Protein Abundance ◽  
Fluorescent Tags ◽  
Exogenous Fluorophores

Genetically encoded fluorescent tags are protein sequences that can be fused to a protein of interest to render it fluorescent. These tags have revolutionized cell biology by allowing nearly any protein to be imaged by light microscopy at submicrometer spatial resolution and subsecond time resolution in a live cell or organism. They can also be used to measure protein abundance in thousands to millions of cells using flow cytometry. Here I provide an introduction to the different genetic tags available, including both intrinsically fluorescent proteins and proteins that derive their fluorescence from binding of either endogenous or exogenous fluorophores. I discuss their optical and biological properties and guidelines for choosing appropriate tags for an experiment. Tools for tagging nucleic acid sequences and reporter molecules that detect the presence of different biomolecules are also briefly discussed.

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