The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions

2011 ◽  
pp. 63-73 ◽  
Author(s):  
Anita Laitinen ◽  
Johanna Nystedt ◽  
Saara Laitinen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Low Oxygen ◽  
Human Cord Blood ◽  
Oxygen Conditions

scholarly journals Derivation of Lung Epithelium from Human Cord Blood–derived Mesenchymal Stem Cells

2008 ◽  
Vol 177 (7) ◽  
pp. 701-711 ◽  
Author(s):  
Viranuj Sueblinvong ◽  
Roberto Loi ◽  
Philip L. Eisenhauer ◽  
Ira M. Bernstein ◽  
Benjamin T. Suratt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Lung Epithelium ◽  
Human Cord Blood

Resveratrol Prevents the Cellular Damages Induced by Monocrotophos via PI3K Signaling Pathway in Human Cord Blood Mesenchymal Stem Cells

2018 ◽  
Vol 55 (11) ◽  
pp. 8278-8292 ◽  
Author(s):  
S. Jahan ◽  
D. Kumar ◽  
S. Singh ◽  
V. Kumar ◽  
A. Srivastava ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Signaling Pathway ◽  
Pi3k Signaling ◽  
Human Cord Blood ◽  
Pi3k Signaling Pathway

Effects of Human Cord Blood Mesenchymal Stem Cells on Cutaneous Wound Healing in Leprdb Mice

2010 ◽  
Vol 65 (6) ◽  
pp. 565-572 ◽  
Author(s):  
Kwan-Chul Tark ◽  
Jong-Won Hong ◽  
Young-Soo Kim ◽  
Seung-Boem Hahn ◽  
Won-Jai Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Cutaneous Wound Healing ◽  
Human Cord Blood ◽  
Cutaneous Wound

Expression of P16 cell cycle inhibitor in human cord blood CD34+ expanded cells following co-culture with bone marrow-derived mesenchymal stem cells

Hematology ◽  
2012 ◽  
Vol 17 (6) ◽  
pp. 334-340 ◽  
Author(s):  
Arezoo Oodi ◽  
Mehrdad Noruzinia ◽  
Mehryar Habibi Roudkenar ◽  
Mahin Nikougoftar ◽  
Mohamad Soleiman Soltanpour ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Human Cord Blood ◽  
Cell Cycle Inhibitor ◽  
Cord Blood Cd34

scholarly journals Transplantation of human cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells in autism

2013 ◽  
Vol 11 (1) ◽  
pp. 196 ◽  
Author(s):  
Yong-Tao Lv ◽  
Yun Zhang ◽  
Min Liu ◽  
Jia-na-ti Qiuwaxi ◽  
Paul Ashwood ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Mononuclear Cells ◽  
Human Cord Blood ◽  
Blood Mononuclear Cells ◽  
Cord Blood Mononuclear Cells

scholarly journals In Vitro Assessment of the Gene Expression Level of EZH-2 and P300 During Motor Neuron Differentiation of Human Cord Blood Mesenchymal Stem Cells

2021 ◽  
pp. 1-19
Author(s):  
Mohammad Taghi Jogataei ◽  
◽  
Faezeh Faghihi ◽  
Marjaneh Motaghed ◽  
Abolfazl Lotfi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Cord Blood ◽  
Regulatory Genes ◽  
Human Cord Blood ◽  
Over Expression ◽  
Significant Expression ◽  
Islet 1

Introduction: The dedication of stem cells for dissociation into a specific type of cell requires the over expression of genes related to a particular phenotype and suppression of the other genes. Through imposing corresponding alterations on the genome, the genome modulators such as transcription factors can be regulated by histone-modifying enzymes. Maintenance of the neurogenesis process depend on the function of some of these genes which can regulate shifting of cells from proliferation to differentiation such as Enhancer of zeste homolog 2 (EZH2) known as an evolutionarily conserved gene. Moreover, motor neurons (MN) in spinal cord can be regulated during neuronal differentiation via one of the histone acetyltransferase (P300). Up until now, the mechanism of epigenetic regulation and gene expression underlie transition process of human cord blood mesenchymal stem cells (hCB-MSCs) into MNs has not been clarified very well. Therefore, the aim of this study was to explore the quantitative expression of MN-related genes including ChAT, Islet-1, and Mnx-1 along with two epigenetic regulatory genes P300 and EZH2 involved in neurogenesis during differentiation of hCB-MSCs into MNs, using two morphogens including Sonic hedgehog (Shh) and Retinoic acid (RA) involved in the specification of MNs during the growth of nervous system. Methods: Flow cytometry was done to characterize the cells (hCB-MSCs). The cells were differentiated into MN-like cells according to our previous procedure using RA (0.01mM) and Shh (100ng/ ml) as the inducing morphogens. CB-MSCs with no treatment were assumed as control cells. RT-qPCR and Immunocytochemistry were performed to find the expression of interested genes in this study. Results: The expression of MN-related markers was confirmed at the level of mRNA and protein by induction of differentiation. The results was confirmed by immunocytochemistry showed that a number of cells about 55.33±15.885% and 49.67±13.796% could express Islet-1 and ChAT, respectively. The level of gene expression of Islet-1 and ChAT was significantly increased at the first and second week of exposure, respectively. After two weeks, expression level of P300 and EZH-2 genes was increased remarkably. No significant expression of Mnx-1 was detected when compared with the control sample. Conclusion: In this study MN-related markers, Islet-1 and ChAT, were detected in differentiated cells of hCB-MSCs supporting the potency of cord blood cells in regeneration of MN-related disorders. The over expression of Islet-1, as an early MN marker, in the presence of Shh and RA indicates the supportive effect of these morphogens for the onset of motor neuron generation. We could also detect significant expression of two potent epigenetic regulatory genes involved in neurogenesis, P300 and EZH2 accompanied by ChAT as the mature motor neuron marker at the second week of exposure due to the elimination of Shh and RA at later time of differentiation. To our knowledge, the evaluation of P300 and EZH2 during differentiation of hCB-MSCs into MN-like cells was performed in this study for the first time. However, the assessment of these epigenetic regulatory genes at the level of protein can be suggested to confirm their functional epigenetic modifying effects during motor neuron differentiation.

scholarly journals Human Cord Blood-Derived CD133+/C-Kit+/Lin− Cells Have Bipotential Ability to Differentiate into Mesenchymal Stem Cells and Outgrowth Endothelial Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Carlos Cardenas ◽  
Ja-Young Kwon ◽  
Yong-Sun Maeng
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Conditioned Medium ◽  
Mononuclear Cells ◽  
Cell Contact ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Human Cord Blood

Recent evidence suggests that mononuclear cells (MNCs) derived from bone marrow and cord blood can differentiate into mesenchymal stem cells (MSCs) or outgrowth endothelial cells (OECs). However, controversy exists as to whether MNCs have the pluripotent capacity to differentiate into MSCs or OECs or are a mixture of cell lineage-determined progenitors of MSCs or OECs. Here, using CD133+/C-kit+/Lin− mononuclear cells (CKL− cells) isolated from human umbilical cord blood using magnetic cell sorting, we characterized the potency of MNC differentiation. We first found that CKL− cells cultured with conditioned medium of OECs or MSCs differentiated into OECs or MSCs and this differentiation was also induced by cell-to-cell contact. When we cultured single CKL− cells on OEC- or MSC-conditioned medium, the cells differentiated morphologically and genetically into OEC- or MSC-like cells, respectively. Moreover, we confirmed that OECs or MSCs differentiated from CKL− cells had the ability to form capillary-like structures in Matrigel and differentiate into osteoblasts, chondrocytes, and adipocytes. Finally, using microarray analysis, we identified specific factors of OECs or MSCs that could potentially be involved in the differentiation fate of CKL− cells. Together, these results suggest that cord blood-derived CKL− cells possess at least bipotential differentiation capacity toward MSCs or OECs.

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