Developing Extrachromosomal Gene Expression Vector Technologies: An Overview

2011 ◽  
pp. 1-17 ◽  
Author(s):  
Richard Wade-Martins
Keyword(s):  
Gene Expression ◽  
Expression Vector

Transient gene expression optimization and expression vector comparison to improve HIV-1 VLP production in HEK293 cell lines

2017 ◽  
Vol 102 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Javier Fuenmayor ◽  
Laura Cervera ◽  
Sonia Gutiérrez-Granados ◽  
Francesc Gòdia
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Expression Vector ◽  
Hek293 Cell ◽  
Transient Gene Expression ◽  
Expression Optimization ◽  
Hiv 1

scholarly journals Construction and Characterization of a Gradually Inducible Expression Vector for Halobacterium salinarum, Based on thekdpPromoter

2012 ◽  
Vol 78 (7) ◽  
pp. 2100-2105 ◽  
Author(s):  
Dorthe Kixmüller ◽  
Jörg-Christian Greie
Keyword(s):  
Gene Expression ◽  
Expression Vector ◽  
Expression System ◽  
Expression Patterns ◽  
Inducible Expression ◽  
Halobacterium Salinarum ◽  
Expression Vectors ◽  
Content Type ◽  
Inducible Expression System

ABSTRACTGradually inducible expression vectors which are governed by variations of growth conditions are powerful tools for gene expression of conditionally lethal mutants. Furthermore, controlled expression allows monitoring of overproduction of proteins at various stages in their expressing hosts. ForHalobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not available to date. Here we show that thekdppromoter (Pkdp), which facilitates gene expression upon K+limitation, can be used to establish such a system for molecular applications. Pkdpfeatures a rather high expression rate, with an approximately 50-fold increase that can be easily varied by K+concentrations in the growth medium. Besides the construction of an expression vector, our work describes the characterization of expression patterns and, thus, offers a gradually inducible expression system to the scientific community.

21 SITE-SPECIFIC RECOMBINATION USING Dre-RECOMBINASE IN PORCINE CELLS AND EMBRYOS

2014 ◽  
Vol 26 (1) ◽  
pp. 125
Author(s):  
S. Y. Yum ◽  
S. J. Kim ◽  
J. H. Moon ◽  
W. J. Choi ◽  
J. H. Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Vector ◽  
Target Gene ◽  
Fluorescent Protein ◽  
Cellular Level ◽  
Cell Stage ◽  
Site Specific ◽  
Target Gene Expression ◽  
Green Fluorescent ◽  
Gateway Cloning System

Site-specific recombinases (SSR), such as Cre and Flp recombinases, which enable DNA excision, insertion, and translocation, have been used for conditional target gene expression in mouse and other vertebrates. In this study, we evaluated another SSR, Dre-recombinase (Dre), which is functionally similar to Cre recombinase in porcine fibroblasts and embryos. For this study, 2 fragment DNA constructs (rox GFP-polyA and rox RFP-polyA) were combined with piggybac transposition expression vector (Kim et al. 2011 J. Vet. Med. Sci.) using a multisite gateway cloning system (MultiSite Gateway® Pro, Invitrogen, Carlsbad, CA, USA). The expression vector carrying rox-flanked green fluorescent protein (GFP) followed by red fluorescent protein (RFP) and transposase were transfected into kidney-derived porcine cells by nucleofection (Neon® Transfection System, Invitrogen). A GFP-expressing cell line, which was not expressing RFP, was established. And then rox-flanked GFP were removed by Dre transfection and RFP was expressed in the kidney cells. At the cellular level, this excision was confirmed by site-specific RT-PCR and sequencing. The rox-flanked GFP cells were reconstructed with enucleated oocytes and then the cloned embryos were cultured in porcine zygote medium-5. Dre was micro-injected into 1 of the 2-cell-stage blastomeres. After 6 days, RFP expression was observed on the part of embryos after microinjection. In conclusion, the data demonstrated that, like other SSR, Dre might be applied in conditional target gene expression for generating porcine biomedical models.

scholarly journals Inhibition of Xhox1A gene expression in Xenopus embryos by antisense RNA produced from an expression vector read by RNA polymerase III

1995 ◽  
Vol 52 (1) ◽  
pp. 37-49 ◽  
Author(s):  
Anthony Nichols ◽  
Elisabeth Rungger-Brändle ◽  
Lisbeth Muster ◽  
Duri Rungger
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Antisense Rna ◽  
Expression Vector ◽  
Rna Polymerase Iii ◽  
Xenopus Embryos ◽  
Polymerase Iii

Cauliflower mosaic virus as a gene expression vector for plants

1990 ◽  
Vol 79 (1) ◽  
pp. 154-157 ◽  
Author(s):  
J. Futterer ◽  
J. M. Bonneville ◽  
T. Hohn
Keyword(s):  
Gene Expression ◽  
Mosaic Virus ◽  
Expression Vector ◽  
Cauliflower Mosaic Virus

scholarly journals The Antisense RNA Approach: a New Application forIn VivoInvestigation of the Stress Response of Oenococcus oeni, a Wine-Associated Lactic Acid Bacterium

2015 ◽  
Vol 82 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Maud Darsonval ◽  
Tarek Msadek ◽  
Hervé Alexandre ◽  
Cosette Grandvalet
Keyword(s):  
Gene Expression ◽  
Lactic Acid ◽  
Stress Response ◽  
Lactic Acid Bacterium ◽  
Antisense Rna ◽  
Expression Vector ◽  
Stress Proteins ◽  
Oenococcus Oeni ◽  
Content Type

ABSTRACTOenococcus oeniis a wine-associated lactic acid bacterium mostly responsible for malolactic fermentation in wine. In wine,O. oenigrows in an environment hostile to bacterial growth (low pH, low temperature, and ethanol) that induces stress response mechanisms. To survive,O. oeniis known to set up transitional stress response mechanisms through the synthesis of heat stress proteins (HSPs) encoded by thehspgenes, notably a unique small HSP named Lo18. Despite the availability of the genome sequence, characterization ofO. oenigenes is limited, and little is known about thein vivorole of Lo18. Due to the lack of genetic tools forO. oeni, an efficient expression vector inO. oeniis still lacking, and deletion or inactivation of thehsp18gene is not presently practicable. As an alternative approach, with the goal of understanding the biological function of theO. oenihsp18genein vivo, we have developed an expression vector to produce antisense RNA targeting ofhsp18mRNA. Recombinant strains were exposed to multiple stresses inducinghsp18gene expression: heat shock and acid shock. We showed that antisense attenuation ofhsp18affectsO. oenisurvival under stress conditions. These results confirm the involvement of Lo18 in heat and acid tolerance ofO. oeni. Results of anisotropy experiments also confirm a membrane-protective role for Lo18, as previous observations had already suggested. This study describes a new, efficient tool to demonstrate the use of antisense technology for modulating gene expression inO. oeni.

An expression vector inhibits gene expression in Xenopus embryos by antisense RNA

1992 ◽  
Vol 201 (6) ◽  
pp. 340-345 ◽  
Author(s):  
Michael Schmid ◽  
Herbert Steinbeisser ◽  
Hans-Henning Epperlein ◽  
Michael F. Trendelenburg ◽  
Hans J. Lipps
Keyword(s):  
Gene Expression ◽  
Antisense Rna ◽  
Expression Vector ◽  
Xenopus Embryos

Efficient control of tetracycline-responsive gene expression from an autoregulated bi-directional expression vector

Gene ◽  
1999 ◽  
Vol 229 (1-2) ◽  
pp. 21-29 ◽  
Author(s):  
Craig A. Strathdee ◽  
Marilyn R. McLeod ◽  
Jennifer R. Hall
Keyword(s):  
Gene Expression ◽  
Expression Vector ◽  
Efficient Control ◽  
Responsive Gene

scholarly journals The Influence of SV40 polyA on Gene Expression of Baculovirus Expression Vector Systems

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145019 ◽  
Author(s):  
Tamer Z. Salem ◽  
Craig P. Seaborn ◽  
Colin M. Turney ◽  
Jianli Xue ◽  
Hui Shang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Vector ◽  
Baculovirus Expression ◽  
Vector Systems ◽  
Baculovirus Expression Vector
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