Characterization of Meiotic Recombination Initiation Sites Using Pulsed-Field Gel Electrophoresis

2011 ◽  
pp. 33-45 ◽  
Author(s):  
Sarah Farmer ◽  
Wing-Kit Leung ◽  
Hideo Tsubouchi
Keyword(s):  
Meiotic Recombination ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Characterization of Staphylococcus intermedius from Pigeons, Dogs, Foxes, Mink, and Horses by Pulsed-Field Gel Electrophoresis

2002 ◽  
Vol 64 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Yoshihisa WAKITA ◽  
Akira SHIMIZU ◽  
Václav HÁJEK ◽  
Junichi KAWANO ◽  
Kenji YAMASHITA
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Staphylococcus Intermedius

scholarly journals Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

1994 ◽  
Vol 113 (1) ◽  
pp. 75-81 ◽  
Author(s):  
F. M. Khambaty ◽  
R. W. Bennett ◽  
D. B. Shah
Keyword(s):  
Gel Electrophoresis ◽  
Pfge Pattern ◽  
Pulsed Field Gel Electrophoresis ◽  
Western United States ◽  
Single Strain ◽  
Pulsed Field ◽  
Definitive Evidence ◽  
Staphylococcus Intermedius ◽  
Epidemiological Characterization

SUMMARYAn outbreak of food intoxication involving over 265 cases in western United States occurred in October 1991.Staphylococcus intermediuswas implicated as the aetiologic agent. Representative outbreak isolates (five clinical and ten from foods) produced type A enterotoxin. DNA fragments generated by four restriction endonucleases and analysed by pulsed-field gel electrophoresis (PFGE) provided definitive evidence that all isolates from nine different counties in California and Nevada were derived from a single strain. The PFGE pattern of these outbreak isolates was distinct from those of a heterogeneous collection of sevenS. intermediusstrains of veterinary origin and five unrelatedS. aureuslaboratory strains. The data show a significant PFGE pattern heterogeneity not only among members of differentStaphylococcusspecies but also within members of the same species and even the same enterotoxin type. The results indicate that PFGE is a valuable strain-specific discriminator for the epidemiological characterization ofS. intermedius. To our knowledge, this represents the first documented foodborne outbreak caused byS. intermedius. These findings suggest that the presence ofS. intermediusand other species such asS. hyicusin food should be reason for concern.

Characterization of Erwinia amylovora Strains from Bulgaria by Pulsed-Field Gel Electrophoresis

2012 ◽  
Vol 67 ◽  
pp. 0187 ◽  
Author(s):  
Iliana Atanasova ◽  
Zoltan Urshev ◽  
Petya Hristova ◽  
Nevena Bogatzevska ◽  
Penka Moncheva
Keyword(s):  
Gel Electrophoresis ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Characterization of Listeria monocytogenes strains isolated from food and humans in Italy by pulsed-field gel electrophoresis

2002 ◽  
Vol 19 (1) ◽  
pp. 47-55 ◽  
Author(s):  
M Gianfranceschi ◽  
M Pourshaban ◽  
A Gattuso ◽  
C Wedell-Neergaard ◽  
P Aureli
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field
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