The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels

2011 ◽  
pp. 7-23 ◽  
Author(s):  
Maria Forlenza ◽  
Thomas Kaiser ◽  
Huub F. J. Savelkoul ◽  
Geert F. Wiegertjes
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Mrna Levels ◽  
Cytokine Mrna ◽  
Real Time Quantitative Pcr

Retinoid receptors in the developing human lung

2002 ◽  
Vol 103 (6) ◽  
pp. 613-621 ◽  
Author(s):  
Yuichiro KIMURA ◽  
Takashi SUZUKI ◽  
Chika KANEKO ◽  
Andrew D. DARNEL ◽  
Takuya MORIYA ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Lung Development ◽  
Thyroid Hormone Receptor ◽  
Fetal Lung ◽  
Mesenchymal Cells ◽  
Mrna Levels ◽  
Adult Lung ◽  
Real Time Quantitative Pcr ◽  
Retinoid Receptors

Nuclear receptors and their ligands are known to play very important roles in lung development. Among these receptors, retinoid receptors, members of the steroid/thyroid hormone receptor superfamily, are classified into retinoic acid receptor (RAR) isoforms α, β, and γ and retinoid X receptor (RXR) isoforms α, β, and γ. In addition, isoforms I and II of the orphan receptor chicken ovalbumin upstream promoter-transcription factor (COUP-TF) have been shown to negatively regulate the activation of retinoid receptors. Both of these receptors have been shown to regulate lung development in the mouse. In the present study we utilized immunohistochemistry and real-time quantitative PCR to examine the expression of RAR-α, -β and -γ, RXR-α, -β and -γ and COUP-TFII in the human fetal lung at 13–16 gestational weeks, a very critical stage of human pulmonary development, in order to study possible roles in pulmonary morphogenesis by comparing these findings with those of the adult lung. RXR-γ immunoreactivity was detected at both proximal (epithelia and mesenchyme of the trachea and bronchi associated with cartilage) and distal (epithelia and mesenchyme of smaller distal bronchi) sites in the fetal lung, but was markedly weaker in the adult lung. RAR-β immunoreactivity was detected in distal mesenchymal cells of the fetal lung, but was not discernible in distal mesenchymal cells in the adult lung (bronchioles, alveolar ducts and alveolus). Relatively intense RAR-γ immunoreactivity was detected in the chondrocytes of bronchial cells. COUP-TFII immunoreactivity was detected with a similar pattern to that of RAR-β. Real-time quantitative PCR analyses revealed that mRNA levels of RXR-γ at proximal and distal sites (ratio of fetal lung/adult lung: 3.4±0.05-fold and 3.1±0.03-fold respectively; P<0.01), RAR-β at distal sites (2.4±0.01-fold; P<0.05) and RAR-γ at proximal sites (2.2±0.11-fold; P<0.05) were significantly higher in the fetus than in the adult.

Evaluation of rat arterial allograft viability by measuring cytokine mRNA using real-time quantitative PCR

2003 ◽  
Vol 35 (1) ◽  
pp. 96-98
Author(s):  
K Nishizaki ◽  
Y Dohi ◽  
T Kiji ◽  
S Nagasaka ◽  
H Sakaguchi ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Cytokine Mrna ◽  
Real Time Quantitative Pcr ◽  
Arterial Allograft

A real-time quantitative PCR comparative study between rat optic and sciatic nerves: determination of neuregulin-1 mRNA levels

2004 ◽  
Vol 130 (1-2) ◽  
pp. 49-60 ◽  
Author(s):  
J.C. Martínez ◽  
C. Malavé ◽  
I. Bosch ◽  
C. Castillo ◽  
J. Núñez ◽  
...  
Keyword(s):  
Comparative Study ◽  
Real Time ◽  
Quantitative Pcr ◽  
Mrna Levels ◽  
Neuregulin 1 ◽  
Real Time Quantitative Pcr ◽  
Sciatic Nerves

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

IFN-γ induced persistent Chlamydia pneumoniae infection in HL and Mono Mac 6 cells: characterization by real-time quantitative PCR and culture

2004 ◽  
Vol 36 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Laura Mannonen ◽  
Eveline Kamping ◽  
Tuula Penttilä ◽  
Mirja Puolakkainen
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Chlamydia Pneumoniae ◽  
Real Time Quantitative Pcr ◽  
Ifn Γ

Analysis of Caecal Microbiota in Rats Fed with Genetically Modified Rice by Real-Time Quantitative PCR

2011 ◽  
Vol 76 (1) ◽  
pp. M88-M93 ◽  
Author(s):  
Wentao Xu ◽  
Liting Li ◽  
Jiao Lu ◽  
YunBo Luo ◽  
Ying Shang ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Genetically Modified ◽  
Genetically Modified Rice ◽  
Real Time Quantitative Pcr ◽  
Caecal Microbiota
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