Qualitative and Quantitative Screening of Amino Acids in Plant Tissues

2012 ◽  
pp. 165-178 ◽  
Author(s):  
Will I. Menzel ◽  
Wen-Ping Chen ◽  
Adrian D. Hegeman ◽  
Jerry D. Cohen
Keyword(s):  
Amino Acids ◽  
Plant Tissues ◽  
Qualitative And Quantitative ◽  
Quantitative Screening

γ-Amino-Butyric Acid and β-Alanine in Plant Tissues: Amino-Acids of the Apple Fruit

Nature ◽  
1950 ◽  
Vol 165 (4201) ◽  
pp. 716-717 ◽  
Author(s):  
A. C. HULME ◽  
W. ARTHINGTON
Keyword(s):  
Amino Acids ◽  
Butyric Acid ◽  
Apple Fruit ◽  
Plant Tissues ◽  
Amino Butyric Acid

scholarly journals HISTOCHEMICAL DIFFERENTIATION BETWEEN TYROSINE AND CHLOROGENIC ACID IN PLANT TISSUES I. NITROUS ACID REACTIONS AND METAL CHELATION OF NITROSOTYROSINE

1968 ◽  
Vol 16 (3) ◽  
pp. 191-198 ◽  
Author(s):  
R. M. REEVE
Keyword(s):  
Amino Acids ◽  
Chlorogenic Acid ◽  
Nitrous Acid ◽  
Metal Chelate ◽  
Ultrastructural Localization ◽  
Plant Tissues ◽  
Metal Chelation ◽  
Copper Chelation ◽  
Acid Reaction ◽  
Color Reactions

Copper chelation of nitrosotyrosine has been found useful for histochemical localization for tyrosine in thick, fresh sections of large celled plant tissues. The nitrous acid reaction for ortho-dihydroxyphenolics also has been found useful for localization of chlorogenic acid, caffeic acid and dihydroxyphenylalanine in plant tissues. Application of these tests separately to serially adjacent sections demonstrated the distribution of tyrosine and chlorogenic acid in different plant tissues. Tests tube reactions on known substances verified specificity and also demonstrated that the presence of other amino acids and phenolics did not interfere with the positive test for tyrosine. The color reactions are sufficiently intense for stereoscopic microscopy and tested sections may be measured photometrically. Further adaptability of the nitrosotyrosine-metal chelate reaction to procedures for ultrastructural localization is suggested.

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