Detection of Leishmania infantum kinetoplast DNA by Real Time PCR in hair of wild rabbits

The study of potential wild mammal reservoirs is necessary for the surveillance of leishmaniosis, as Leishmania protozoans have been isolated from a wide range of wild and domestic animal species, including Leporidae. Recently, it has been demonstrated that both hares and wild rabbits can act as sylvatic reservoirs of Leishmania. In Spain, most of the research involving wild rabbits has been developed in the central area of Madrid and in the southeastern Mediterranean coast. We studied the presence of Leishmania infantum in 116 wild rabbits (Oryctolagus cuniculus) captured in Santovenia de Pisuerga, Valladolid, Spain. Hair samples were analyzed by real time PCR. L. infantum kinetoplast DNA (kDNA) was detected and quantified in 4 out of 116 analyzed animals. The estimated number of parasites obtained were quite variable, ranging from 2.60 to 276.60. Hair samples can be collected by non-invasive methods, being a proper sample for Leishmania detection in wild Leporidae, which have an important role as reservoirs of Leishmania. Our findings enhance the need for more extensive studies in different geographical areas.

Marina el-Alamein as an Example of Painting Decoration of Main Spaces of Hellenistic-Roman Houses in Egypt

The article is based on the research conducted by the authors. The houses from the ancient town discovered in 1985 on the Mediterranean coast at the location of today’s Marina el-Alamein, are among the rare remains of Egyptian residential buildings from Hellenistic and Roman times. There are few remains of houses from this period in major cities, including the capital of Alexandria. The ancient town, which functioned under the influence of nearby Alexandria, developed from the second century BC to the sixth century AD. Various types of buildings, relatively well preserved here, provide information on a reduced scale about the architecture of Alexandria, as well as the lives of its inhabitants. This also applies in particular to residential houses, their décor and colours. The ancient town of Marina el-Alamein can be seen as demonstrating solutions that are more common. The present article aims to analyse the preserved remains of painting decoration in the main spaces of houses and attempts to reconstruct forms and the principles of their creation on the background of better-known solutions from other regions. Houses in Marina generally implemented layouts defined by flagstone-paved portico courtyards, sporadically taking the form of an incomplete peristyle, and reception halls oikos, which could be accompanied by smaller adjoining rooms. Both the columns and entablature with the cornices of the porticoes as well as the walls of the main rooms were painted. Wall decoration was organised by geometric partitions, filled in variously. The aedicula that served religious purposes, placed centrally in the rear walls of the main reception rooms, was also polychrome. The painted decoration can be reconstructed from the preserved remains, as rich and intensely colourful, similar to Hellenistic and Roman layouts from other regions, but differing in details.

Medicinal-Cosmetic Potential of the Local Endemic Plants of Crete (Greece), Northern Morocco and Tunisia: Priorities for Conservation and Sustainable Exploitation of Neglected and Underutilized Phytogenetic Resources

Medicinal-aromatic plants (MAPs) are important sources for the development of new valuable products of interest to human and animal health, and are also used as ornamentals for the horticulture industry. However, the increased global demand and the uncontrolled exploitation of these plants constitute a threat to their sustainability. To date, few scientific investigations have focused on MAPs valorization and their domestication. The purpose of this study was to evaluate for the first time the medicinal-cosmetic potential of 399 local endemic Mediterranean plants confined to Crete (223 taxa), the Mediterranean coast-Rif of Morocco (94), and Tunisia (82). The new methodological scheme was developed by experts through three multidisciplinary co-creative workshops and was adjusted by end-users to point-scoring of nine attributes evaluating the potential of the targeted neglected and underutilized plants (NUPs) in the medicinal-cosmetic sector. The results were demonstrated as percentage of the maximum possible score. These assessments were further linked and discussed with respect to feasibility and readiness timescale evaluations for sustainable exploitation of the focal NUPs. A great diversity of local endemic NUPs (30 taxa, 11 families) were associated with interesting medicinal-cosmetic properties (>35% up to 94.44%). Among them, 8 taxa showed the highest medicinal-cosmetic potential (>55% of maximum possible score), half of which are threatened with extinction. Although ex-situ conservation efforts and applied research work are needed to safeguard and unlock the full potential of the local endemic NUPs evaluated herein, the proposed multifaceted evaluation scheme revealed that some local endemic NUPs of the studied regions can be sustainably exploited in short- or medium-term, following successful examples of Cretan NUPs e.g., Origanum dictramnus. The sustainable exploitation of high scored taxa of the studied regions can be fastened through targeted species-specific research bridging extant research gaps and facilitating conservation and stakeholder attraction.

Assessment of the Coastal Sensitivity Using the CS Index in the Gulf of Gabes Tunisia; Southern Mediterranean Coast

Assessment of the coastal degradation using the coastal sensitivity index (CSI) in the microtidal environment (Gulf of Gabes, Tunisia) shows that 78.6% of the coast is under low to moderate sensitivity. Moderate sensitivity class includes the sheltered zones and the stable sea cliffs. The highest sensitivity was recorded in sandy beaches (12.7%) where the drift actions amplify littoral fragility and flood risk.The coastal sensitivity index (CSI) is resulted from the combination of eight ranked variables: (a) geomorphology (b) coastal slope (c) hinterland topography (d) facies (e) shoreline exposure (f) shoreline changes (g) tide (h) sea-level trend. Fifty six morphodynamic cells are deduced from a strictmorphologic classification of the studied coast. Each specified cell presents a numerical pattern according to the ranked physical and structural variables. Once managed, the beaches with the highest sensitivity are immediately disturbed. Littoral disturbance is irreversible when the foredune unit is damaged but sustainable remediation of coastal areas must emphasize with the adequate planning for the environmental rehabilitation.

Larvae ecology and adult activity of Aedes mariae (Diptera: Culicidae) in a touristic rock-pool area of the Balearic Islands (Western Mediterranean)

Mosquitoes are vectors of several diseases of medical concern such as malaria or dengue and can also negatively affect tourism and the life-quality of the neighbourhood. The species Aedes mariae (Sergent and Sergent, 1903) is a poorly studied mosquito that breeds in rock-pools of the Mediterranean coast. General Linear Mixed Models (GLMM) were used to determine drivers affecting the presence and abundance of this species. Abiotic and biotic factors were recorded in rock-pools with the presence of Ae. mariae sub-adults across a supralittoral area of Majorca Island (Balearic Islands, Spain) from July 2018 to June 2019. We tested how abiotic factors affected the presence of larvae, while the biotic factors were used to check their effect on larvae abundance. human landing collection was also conducted to assess the adult activity of this species. Valuable data were recorded to improve our knowledge about the bioecology of Ae. mariae in a touristic area of the island of Majorca. Salinity and pH were the most explanatory variables for the presence of Ae. mariae larvae. The presence of Posidonia oceanica (L.) Delile 1813 leaves negatively affected the abundance of Ae. mariae larvae while the presence of other fauna enhanced it. Adult females of Ae. mariae were active for 26 min after sunset in June and its host-seeking activity decreased during autumn months. Control methods against this species should be focussed on rock-pools and planning treatments according to tides, waves and precipitation.

Crust Biometry of Hydrolithon Boreale (Rhodophyta: Hydrolithaceae) on Leave of Posidonia Oceanica Along a Mediterranean Coast Fed by Siliciclastic-Carbonate

A study was conducted to estimate biometry of an epiphytic micro-calcareous red algae, Hydrolithon boreale found on leaves of a Mediterranean meadow, Posidonia oceanica along the entire Turkish coast of the Mediterranean Sea in time (winter and summer 2019) and space (regions, and bottom type and depth). Percent occurrence of the epiphyte was lower in winter (25%), particularly found in a small bay in the west than summer (44%), entirely along the coast. The epiphyte grew up to 5 mm in diameter, 0.35 mm in thickness of the crust size, and was populated up to 1006 ind/m2 in summer owing to the increased utilization of the carbonate by the epiphyte with the increased water temperature. The size was contrasted to the density (abundance and biomass) in space. The biometry was significantly dependent on the siliciclastic-carbonate deposition as inferred from SiO2 of the water in relation the leaf area index (LAI) of P. oceanica. Therefore, this deposition induced specimens growing in size, followed by the reduced density in relation to N-based nutrient of the water. Further major environmental parameters which negatively affected the biometry were pH and total suspended matter of the water, analogous to the turbidity. Of the trace elements, Ni was negatively correlated with the biometry whereas the LAI was however positively correlated with all the anthropogenic-sourced trace elements (V, Cu, Zn, As, Cd, and Pb) in the leaves. Of the bottom types, the calcite rock had higher density than the other soft bottoms in contrast to the size of the epiphyte.