Calcium Imaging of Neural Activity in the Olfactory System of Drosophila

2012 ◽  
pp. 43-70 ◽  
Author(s):  
Antonia Strutz ◽  
Thomas Völler ◽  
Thomas Riemensperger ◽  
André Fiala ◽  
Silke Sachse
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Olfactory System

Learning Compact DNN Models for Behavior Prediction from Neural Activity of Calcium Imaging

2021 ◽  
Author(s):  
Xiaomin Wu ◽  
Da-Ting Lin ◽  
Rong Chen ◽  
Shuvra S. Bhattacharyya
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Behavior Prediction

scholarly journals Habituation as a neural algorithm for online odor discrimination

2020 ◽  
Vol 117 (22) ◽  
pp. 12402-12410 ◽  
Author(s):  
Yang Shen ◽  
Sanjoy Dasgupta ◽  
Saket Navlakha
Keyword(s):  
Neural Activity ◽  
Olfactory System ◽  
Simple Algorithm ◽  
Fruit Fly ◽  
Neural Systems ◽  
Redundant Information ◽  
Biological Mechanism ◽  
Sensory Physiology ◽  
Odor Perception ◽  
Odor Mixtures

Habituation is a form of simple memory that suppresses neural activity in response to repeated, neutral stimuli. This process is critical in helping organisms guide attention toward the most salient and novel features in the environment. Here, we follow known circuit mechanisms in the fruit fly olfactory system to derive a simple algorithm for habituation. We show, both empirically and analytically, that this algorithm is able to filter out redundant information, enhance discrimination between odors that share a similar background, and improve detection of novel components in odor mixtures. Overall, we propose an algorithmic perspective on the biological mechanism of habituation and use this perspective to understand how sensory physiology can affect odor perception. Our framework may also help toward understanding the effects of habituation in other more sophisticated neural systems.

scholarly journals Calcium Imaging in the Drosophila Olfactory System with a Genetic Indicator

2013 ◽  
Vol 2013 (11) ◽  
pp. pdb.prot078568-pdb.prot078568
Author(s):  
C. M. Root ◽  
A. M. Wong ◽  
J. Flores ◽  
J. W. Wang
Keyword(s):  
Calcium Imaging ◽  
Olfactory System

scholarly journals Cognitive experience alters cortical involvement in navigation decisions

2021 ◽  
Author(s):  
Christopher D Harvey ◽  
Charlotte Arlt ◽  
Roberto Barroso-Luque ◽  
Shinichiro Kira ◽  
Carissa A Bruno ◽  
...  
Keyword(s):  
Decision Making ◽  
Neural Activity ◽  
Calcium Imaging ◽  
Posterior Parietal Cortex ◽  
Retrosplenial Cortex ◽  
Neuron Activity ◽  
Decision Task ◽  
Simple Task ◽  
Critical Determinant ◽  
Cortical Areas

The neural correlates of decision-making have been investigated extensively, and recent work aims to identify under what conditions cortex is actually necessary for making accurate decisions. We discovered that mice with distinct cognitive experiences, beyond sensory and motor learning, use different cortical areas and neural activity patterns to solve the same task, revealing past learning as a critical determinant of whether cortex is necessary for decision-making. We used optogenetics and calcium imaging to study the necessity and neural activity of multiple cortical areas in mice with different training histories. Posterior parietal cortex and retrosplenial cortex were mostly dispensable for accurate decision-making in mice performing a simple navigation-based decision task. In contrast, these areas were essential for the same simple task when mice were previously trained on complex tasks with delay periods or association switches. Multi-area calcium imaging showed that, in mice with complex-task experience, single-neuron activity had higher selectivity and neuron-neuron correlations were weaker, leading to codes with higher task information. Therefore, past experience sets the landscape for how future tasks are solved by the brain and is a key factor in determining whether cortical areas have a causal role in decision-making.

scholarly journals Interpreting wide-band neural activity using convolutional neural networks

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Markus Frey ◽  
Sander Tanni ◽  
Catherine Perrodin ◽  
Alice O'Leary ◽  
Matthias Nau ◽  
...  
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Wide Band ◽  
Brain Regions ◽  
Imaging Data ◽  
User Input ◽  
Neural Recordings ◽  
Learning Framework ◽  
Behavioral Variables ◽  
Spatial Behaviors

Rapid progress in technologies such as calcium imaging and electrophysiology has seen a dramatic increase in the size and extent of neural recordings. Even so, interpretation of this data requires considerable knowledge about the nature of the representation and often depends on manual operations. Decoding provides a means to infer the information content of such recordings but typically requires highly processed data and prior knowledge of the encoding scheme. Here, we developed a deep-learning framework able to decode sensory and behavioral variables directly from wide-band neural data. The network requires little user input and generalizes across stimuli, behaviors, brain regions, and recording techniques. Once trained, it can be analyzed to determine elements of the neural code that are informative about a given variable. We validated this approach using electrophysiological and calcium-imaging data from rodent auditory cortex and hippocampus as well as human electrocorticography (ECoG) data. We show successful decoding of finger movement, auditory stimuli, and spatial behaviors – including a novel representation of head direction - from raw neural activity.

scholarly journals All-optical imaging and patterned stimulation with a one-photon endoscope

2021 ◽  
Author(s):  
Jinyong Zhang ◽  
Ryan N Hughes ◽  
Namsoo Kim ◽  
Isabella P Fallon ◽  
Konstantin I bakhurin ◽  
...  
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Neural Circuit ◽  
Integrated System ◽  
Striatal Neurons ◽  
Indirect Pathway ◽  
Cellular Resolution ◽  
All Optical ◽  
Freely Moving

While in vivo calcium imaging makes it possible to record activity in defined neuronal populations with cellular resolution, optogenetics allows selective manipulation of neural activity. Recently, these two tools have been combined to stimulate and record neural activity at the same time, but current approaches often rely on two-photon microscopes that are difficult to use in freely moving animals. To address these limitations, we have developed a new integrated system combining a one-photon endoscope and a digital micromirror device for simultaneous calcium imaging and precise optogenetic photo-stimulation with near cellular resolution (Miniscope with All-optical Patterned Stimulation and Imaging, MAPSI). Using this highly portable system in freely moving mice, we were able to image striatal neurons from either the direct pathway or the indirect pathway while simultaneously activating any neuron of choice in the field of view, or to synthesize arbitrary spatiotemporal patterns of photo-stimulation. We could also select neurons based on their relationship with behavior and recreate the behavior by mimicking the natural neural activity with photo-stimulation. MAPSI thus provides a powerful tool for interrogation of neural circuit function in freely moving animals.

Chaotic itinerancy: Insufficient perceptual evidence

2001 ◽  
Vol 24 (5) ◽  
pp. 819-820 ◽  
Author(s):  
Leslie M. Kay
Keyword(s):  
Information Processing ◽  
Neural Activity ◽  
Olfactory System ◽  
Attractor Dynamics ◽  
Large Step ◽  
Perceptual Evidence ◽  
Cantor Coding

Chaotic itinerancy is useful for illustrating transitions in attractor dynamics seen in the olfactory system. Cantor coding is a good model for information processing, but so far it lacks perceptual proof. The theories presented provide a large step toward bridging the use of chaos as an interpretive tool and hard examination of chaotic neural activity during perception.

Non-invasive in vivo functional optoacoustic calcium imaging of neural activity in GCaMP6f-expressing mice

2017 ◽  
Author(s):  
Sven Gottschalk ◽  
Xose Luis Deán-Ben ◽  
Shy Shoham ◽  
Daniel Razansky
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Non Invasive

scholarly journals Simultaneous multi-electrode array recording and two-photon calcium imaging of neural activity

2010 ◽  
Vol 192 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Woodrow L. Shew ◽  
Timothy Bellay ◽  
Dietmar Plenz
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Electrode Array ◽  
Two Photon ◽  
Multi Electrode Array
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