High-Throughput Data Pipelines for Metabolic Flux Analysis in Plants

2013 ◽  
pp. 223-246 ◽  
Author(s):  
C. Hart Poskar ◽  
Jan Huege ◽  
Christian Krach ◽  
Yair Shachar-Hill ◽  
Björn H. Junker
Keyword(s):  
High Throughput ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
High Throughput Data

scholarly journals State-of-the-Art Genetic Modalities to Engineer Cyanobacteria for Sustainable Biosynthesis of Biofuel and Fine-Chemicals to Meet Bio–Economy Challenges

Life ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 54 ◽  
Author(s):  
Aqib Zafar Khan ◽  
Muhammad Bilal ◽  
Shahid Mehmood ◽  
Ashutosh Sharma ◽  
Hafiz M. N. Iqbal
Keyword(s):  
High Throughput ◽  
Metabolic Flux Analysis ◽  
Large Scale ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Fine Chemicals ◽  
Carbon Regulation ◽  
Regulation Mechanisms ◽  
Large Scale Cultivation ◽  
Genome Scale

In recent years, metabolic engineering of microorganisms has attained much research interest to produce biofuels and industrially pertinent chemicals. Owing to the relatively fast growth rate, genetic malleability, and carbon neutral production process, cyanobacteria has been recognized as a specialized microorganism with a significant biotechnological perspective. Metabolically engineering cyanobacterial strains have shown great potential for the photosynthetic production of an array of valuable native or non-native chemicals and metabolites with profound agricultural and pharmaceutical significance using CO2 as a building block. In recent years, substantial improvements in developing and introducing novel and efficient genetic tools such as genome-scale modeling, high throughput omics analyses, synthetic/system biology tools, metabolic flux analysis and clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease (CRISPR/cas) systems have been made for engineering cyanobacterial strains. Use of these tools and technologies has led to a greater understanding of the host metabolism, as well as endogenous and heterologous carbon regulation mechanisms which consequently results in the expansion of maximum productive ability and biochemical diversity. This review summarizes recent advances in engineering cyanobacteria to produce biofuel and industrially relevant fine chemicals of high interest. Moreover, the development and applications of cutting-edge toolboxes such as the CRISPR-cas9 system, synthetic biology, high-throughput “omics”, and metabolic flux analysis to engineer cyanobacteria for large-scale cultivation are also discussed.

scholarly journals iMS2Flux – a high–throughput processing tool for stable isotope labeled mass spectrometric data used for metabolic flux analysis

2012 ◽  
Vol 13 (1) ◽  
pp. 295 ◽  
Author(s):  
C Hart Poskar ◽  
Jan Huege ◽  
Christian Krach ◽  
Mathias Franke ◽  
Yair Shachar-Hill ◽  
...  
Keyword(s):  
Stable Isotope ◽  
High Throughput ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Mass Spectrometric ◽  
Flux Analysis ◽  
Mass Spectrometric Data ◽  
Spectrometric Data

Towards high throughput metabolic flux analysis in plants

2012 ◽  
Vol 8 (10) ◽  
pp. 2466 ◽  
Author(s):  
Jan Huege ◽  
C. Hart Poskar ◽  
Mathias Franke ◽  
Björn H. Junker
Keyword(s):  
High Throughput ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis
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