Stem Cell Spheres for Corneal Regeneration

2019 ◽  
pp. 299-316 ◽  
Author(s):  
Salim Ismail ◽  
Jennifer J. McGhee ◽  
Ye Li ◽  
Jeremy John Mathan ◽  
Jinny Jung Yoon ◽  
...  
Keyword(s):  
Stem Cell ◽  
Corneal Regeneration

Limbal Epithelial and Mesenchymal Stem Cell Therapy for Corneal Regeneration

2019 ◽  
Vol 45 (3) ◽  
pp. 265-277 ◽  
Author(s):  
Sachin Shukla ◽  
Swapna S Shanbhag ◽  
Fatemeh Tavakkoli ◽  
Shobhit Varma ◽  
Vivek Singh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Mesenchymal Stem Cell Therapy ◽  
Corneal Regeneration

scholarly journals ESTIMATION OF THE EFFICIENCY MESENCHYMAL STEM CELL SECRETOME USING IN POST-OPERATIVE CORNEAL SEQUESTRUM THERAPY CLINICAL TREATMENT RESULTS

2021 ◽  
Vol 248 (4) ◽  
pp. 196-203
Author(s):  
S.V. Saroyan ◽  
◽  
S.V. Saroyan ◽  
◽  
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Mesenchymal Stem Cell ◽  
Clinical Treatment ◽  
Donor Cornea ◽  
Treatment Results ◽  
Post Operative Period ◽  
Corneal Regeneration ◽  
Positive Effect

This article summarizes our clinical treatment results for various stages of corneal sequestrum in 24 cats using mesenchymal stem cell secretome (cytokines / growth factors). Our results show that the regenerative drug used in the study had a positive effect on the quality and speed of the corneal regeneration and effectively reduced inflammation during the post-operative period, which contributed to the lack of any rejection of donor cornea.

scholarly journals COMPARATIVE STUDY OF THE EFFICACY OF STEM CELLS IN CORNEAL REGENERATION IN A CHEMICAL BURN IN RABBITS

2020 ◽  
Author(s):  
Abdelkrim Salama ◽  
Abdul-Malik Abudunia ◽  
Ali Gamal Al-kaf ◽  
Nabeel Muhsin Ziad ◽  
Azeddine Ibrahimi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Peer Review ◽  
Cell Transplantation ◽  
Cell Transplant ◽  
Chemical Burn ◽  
Limbal Stem Cells ◽  
Corneal Regeneration ◽  
E Mail

Objectives: This study compares the efficacy of stem cell transplantation in corneal regeneration and restoration of the limbic deficit in an experimental chemical burn in rabbits. Methods: Biopsy was performed of the limbus and the chemical burns for all rabbits, and we collected the amniotic membranes from a pregnant female rabbit. We kept a control group without transplantation, to study spontaneous and natural healing, and we transplanted the stem cells produced in vitro under the corneal epithelium burned. To compare the result, we tested a group for amniotic stem cell transplantation, a group for limbal stem cell graft, and another group for combined transplantation of both types of stem cells. Results: Transplanted rabbits develop permanent unilateral blindness due to a severe limbic deficit. The group receiving only amniotic stem cells shows temporary anatomical improvement without functional recovery. The two groups receiving limbal stem cells alone or combined with amniotic stem cells showed anatomical and functional satisfaction with quick recovery time for the combined transplantation. Conclusions: A simple chemical burn can establish permanent blindness. When the limbic deficit is important, spontaneous healing is not available. Transplantation of stem cell transplant is the only way to repair this deficit and regenerate the cornea. Only limbic stem cells can be sufficient. Amniotic stem cells can support and speed up the healing time when it combined to limbal stem cells graft.  Peer Review History: Received 23 July 2020; Revised 14 August; Accepted 28 August, Available online 15 September 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Name: Dr. Mohamed Amin El-Emam Affiliation: Department of Pharmacology and Therapeutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria (PUA), Alexandria, Egypt. E-mail: [email protected]   Name: Francesco Ferrara Affiliation: USL Umbria 1, Perugia, Italy E-mail: [email protected]   Name: Maged Almezgagi Affiliation: Department of Immunology, Medical College of Qinghai University, Qinghai Xining 810001, China E-mail: [email protected]   Name: Dr. Asia Selman Abdullah Affiliation: University of Basrah, Iraq E-mail: [email protected]   Comments of reviewer(s):

scholarly journals Bi-compartmentalized stem cell organization of the corneal limbal niche

2020 ◽  
Author(s):  
Olivia Farrelly ◽  
Yoko Suzuki-Horiuchi ◽  
Megan Brewster ◽  
Paola Kuri ◽  
Sixia Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Tissue Architecture ◽  
Corneal Regeneration ◽  
Cell Organization ◽  
Cell Niche ◽  
Slow Cycling ◽  
And Function

AbstractStem cells exist in precise locations within tissues, yet how their organization supports tissue architecture and function is poorly understood. The limbus is the presumptive stem cell niche of the corneal epithelium. Here, we visualize the live limbus and track the activity of single stem cells in their native environment by 2-photon microscopy. We identify previously unknown niche compartments and show that long implicated slow-cycling cells form separate lineages in the outer limbus, with only local clonal dynamics. Instead, we find distinct stem cells in the pericorneal limbus to be required for corneal regeneration. Unbiased photolabeling captures their progeny exiting the niche, then moving centripetally in unison before undergoing terminal differentiation. This study demonstrates how a compartmentalized stem cell organization coordinates tissue regeneration.One Sentence SummaryIn vivo live imaging of the regenerating cornea reveals distinct stem cell activities in the limbal niche

Stem Cell Applications in Corneal Regeneration and Wound Repair

2017 ◽  
pp. 213-255
Author(s):  
Steffi Matthyssen ◽  
Bert Van den Bogerd ◽  
Sorcha Ní Dhubhghaill ◽  
Carina Koppen ◽  
Nadia Zakaria
Keyword(s):  
Stem Cell ◽  
Wound Repair ◽  
Corneal Regeneration

Stem Cell Therapy for Corneal Regeneration Medicine and Contemporary Nanomedicine for Corneal Disorders

2015 ◽  
Vol 24 (10) ◽  
pp. 1915-1930 ◽  
Author(s):  
Chih-Chien Hsu ◽  
Chi-Hsien Peng ◽  
Kuo-Hsuan Hung ◽  
Yi-Yen Lee ◽  
Tai-Chi Lin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Regeneration Medicine ◽  
Corneal Regeneration

Stem cell–based therapeutic approaches toward corneal regeneration

2020 ◽  
pp. 263-294
Author(s):  
Balu Venugopal ◽  
Bernadette K. Madathil ◽  
Anil Kumar P.R.
Keyword(s):  
Stem Cell ◽  
Therapeutic Approaches ◽  
Corneal Regeneration

scholarly journals Limbal Stem-Cell Therapy and Long-Term Corneal Regeneration

2010 ◽  
Vol 363 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Paolo Rama ◽  
Stanislav Matuska ◽  
Giorgio Paganoni ◽  
Alessandra Spinelli ◽  
Michele De Luca ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Corneal Regeneration ◽  
Limbal Stem Cell

scholarly journals Corneal Stem Cells as a Source of Regenerative Cell-Based Therapy

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Jasmin S. Nurković ◽  
Radiša Vojinović ◽  
Zana Dolićanin
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Therapeutic Potential ◽  
Corneal Stroma ◽  
Epithelial Stem Cells ◽  
Corneal Surface ◽  
Cell Based Therapy ◽  
Corneal Regeneration ◽  
Successful Transplantation ◽  
Stromal Stem Cells

In the past few years, intensive research has focused on corneal stem cells as an unlimited source for cell-based therapy in regenerative ophthalmology. Today, it is known that the cornea has at least two types of stem cells: limbal epithelial stem cells (LESCs) and corneal stromal stem cells (CSSCs). LESCs are used for regeneration of corneal surface, while CSSCs are used for regeneration of corneal stroma. Until now, various approaches and methods for isolation of LESCs and CSSCs and their successful transplantation have been described and tested in several preclinical studies and clinical trials. This review describes in detail phenotypic characteristics of LESCs and CSSCs and discusses their therapeutic potential in corneal regeneration. Since efficient and safe corneal stem cell-based therapy is still a challenging issue that requires continuous cooperation between researchers, clinicians, and patients, this review addresses the important limitations and suggests possible strategies for improvement of corneal stem cell-based therapy.

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