scholarly journals ESTIMATION OF THE EFFICIENCY MESENCHYMAL STEM CELL SECRETOME USING IN POST-OPERATIVE CORNEAL SEQUESTRUM THERAPY CLINICAL TREATMENT RESULTS

2021 ◽  
Vol 248 (4) ◽  
pp. 196-203
Author(s):  
S.V. Saroyan ◽  
◽  
S.V. Saroyan ◽  
◽  
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Mesenchymal Stem Cell ◽  
Clinical Treatment ◽  
Donor Cornea ◽  
Treatment Results ◽  
Post Operative Period ◽  
Corneal Regeneration ◽  
Positive Effect

This article summarizes our clinical treatment results for various stages of corneal sequestrum in 24 cats using mesenchymal stem cell secretome (cytokines / growth factors). Our results show that the regenerative drug used in the study had a positive effect on the quality and speed of the corneal regeneration and effectively reduced inflammation during the post-operative period, which contributed to the lack of any rejection of donor cornea.

scholarly journals Incorporating β-cyclodextrin into collagen scaffolds to sequester growth factors and modulate mesenchymal stem cell activity

2018 ◽  
Vol 76 ◽  
pp. 116-125 ◽  
Author(s):  
William K. Grier ◽  
Aleczandria S. Tiffany ◽  
Matthew D. Ramsey ◽  
Brendan A.C. Harley
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Mesenchymal Stem Cell ◽  
Cell Activity ◽  
Collagen Scaffolds ◽  
Stem Cell Activity

Limbal Epithelial and Mesenchymal Stem Cell Therapy for Corneal Regeneration

2019 ◽  
Vol 45 (3) ◽  
pp. 265-277 ◽  
Author(s):  
Sachin Shukla ◽  
Swapna S Shanbhag ◽  
Fatemeh Tavakkoli ◽  
Shobhit Varma ◽  
Vivek Singh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Mesenchymal Stem Cell Therapy ◽  
Corneal Regeneration

Polysaccharide-Based Polyelectrolyte Multilayer Surface Coatings can Enhance Mesenchymal Stem Cell Response to Adsorbed Growth Factors

2010 ◽  
Vol 11 (10) ◽  
pp. 2629-2639 ◽  
Author(s):  
Jorge Almodóvar ◽  
Samantha Bacon ◽  
Jarrod Gogolski ◽  
John D. Kisiday ◽  
Matt J. Kipper
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Mesenchymal Stem Cell ◽  
Cell Response ◽  
Surface Coatings ◽  
Polyelectrolyte Multilayer

scholarly journals The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5683
Author(s):  
A. Thirumal Raj ◽  
Supriya Kheur ◽  
Zohaib Khurshid ◽  
Mohammed E. Sayed ◽  
Maryam H. Mugri ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Oral Cancer ◽  
Mesenchymal Stem Cell ◽  
Cancer Cells ◽  
Dental Pulp ◽  
Inflammatory Cytokine ◽  
Ki 67 ◽  
Oral Carcinogenesis ◽  
Oral Cancer Cells

Background: Growth factors and cytokines responsible for the regenerative potential of the dental pulp mesenchymal stem cell secretome (DPMSC-S) are implicated in oral carcinogenesis. The impact and effects of these secretory factors on cancer cells must be understood in order to ensure their safe application in cancer patients. Objective: We aimed to quantify the growth factors and cytokines in DPMSC-S and assess their effect on oral cancer cell proliferation. Materials and methods: DPMSCs were isolated from patients with healthy teeth (n = 5) that were indicated for extraction for orthodontic reasons. The cells were characterized using flow cytometry and conditioned medium (DPMSC-CM) was prepared. DPMSC-CM was subjected to a bead-based array to quantify the growth factors and cytokines that may affect oral carcinogenesis. The effect of DPMSC-CM (20%, 50%, 100%) on the proliferation of oral cancer cells (AW123516) was evaluated using a Ki-67-based assay at 48 h. AW13516 cultured in the standard growth medium acted as the control. Results: VEGF, HCF, Ang-2, TGF-α, EPO, SCF, FGF, and PDGF-BB were the growth factors with the highest levels in the DPMSC-CM. The highest measured pro-inflammatory cytokine was TNF-α, followed by CXCL8. The most prevalent anti-inflammatory cytokine in the DPMSC-CM was IL-10, followed by TGF-β1 and IL-4. Concentrations of 50% and 100% DPMSC-CM inhibited Ki-67 expression in AW13516, although the effect was non-significant. Moreover, 20% DPMSC-CM significantly increased Ki-67 expression compared to the control. Conclusions: The increased Ki-67 expression of oral cancer cells in response to 20% DPMSC-CM indicates the potential for cancer progression. Further research is needed to identify their effects on other carcinogenic properties, including apoptosis, stemness, migration, invasion, adhesion, and therapeutic resistance.

scholarly journals Soluble matrix protein is a potent modulator of mesenchymal stem cell performance

2019 ◽  
Vol 116 (6) ◽  
pp. 2042-2051 ◽  
Author(s):  
Giselle C. Yeo ◽  
Anthony S. Weiss
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Growth Factor ◽  
Mesenchymal Stem Cell ◽  
Wound Repair ◽  
Matrix Protein ◽  
Matrix Proteins ◽  
Extracellular Matrix Protein ◽  
Proliferative Potential ◽  
Potent Growth

We challenge the conventional designation of structural matrix proteins primarily as supporting scaffolds for resident cells. The extracellular matrix protein tropoelastin is classically regarded as a structural component that confers mechanical strength and resilience to tissues subject to repetitive elastic deformation. Here we describe how tropoelastin inherently induces a range of biological responses, even in cells not typically associated with elastic tissues and in a manner unexpected of typical substrate-dependent matrix proteins. We show that tropoelastin alone drives mesenchymal stem cell (MSC) proliferation and phenotypic maintenance, akin to the synergistic effects of potent growth factors such as insulin-like growth factor 1 and basic fibroblast growth factor. In addition, tropoelastin functionally surpasses these growth factors, as well as fibronectin, in allowing substantial media serum reduction without loss of proliferative potential. We further demonstrate that tropoelastin elicits strong mitogenic and cell-attractive responses, both as an immobilized substrate and as a soluble additive, via direct interactions with cell surface integrins αvβ3 and αvβ5. This duality of action converges the long-held mechanistic dichotomy between adhesive matrix proteins and soluble growth factors and uncovers the powerful, untapped potential of tropoelastin for clinical MSC expansion and therapeutic MSC recruitment. We propose that the potent, growth factor-like mitogenic and motogenic abilities of tropoelastin are biologically rooted in the need for rapid stem cell homing and proliferation during early development and/or wound repair.

scholarly journals Combinations of growth factors for human mesenchymal stem cell proliferation and osteogenic differentiation

2020 ◽  
Vol 9 (7) ◽  
pp. 412-420 ◽  
Author(s):  
Veronika Hefka Blahnova ◽  
Jana Dankova ◽  
Michala Rampichova ◽  
Eva Filova
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Growth Factors ◽  
Growth Factor ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cellular Metabolism ◽  
Human Mesenchymal Stem Cell ◽  
Mesodermal Cell ◽  
Alp Activity

Aims Here we introduce a wide and complex study comparing effects of growth factors used alone and in combinations on human mesenchymal stem cell (hMSC) proliferation and osteogenic differentiation. Certain ways of cell behaviour can be triggered by specific peptides – growth factors, influencing cell fate through surface cellular receptors. Methods In our study transforming growth factor β (TGF-β), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF) were used in order to induce osteogenesis and proliferation of hMSCs from bone marrow. These cells are naturally able to differentiate into various mesodermal cell lines. Effect of each factor itself is pretty well known. We designed experimental groups where two and more growth factors were combined. We supposed cumulative effect would appear when more growth factors with the same effect were combined. The cellular metabolism was evaluated using MTS assay and double-stranded DNA (dsDNA) amount using PicoGreen assay. Alkaline phosphatase (ALP) activity, as early osteogenesis marker, was observed. Phase contrast microscopy was used for cell morphology evaluation. Results TGF-β and bFGF were shown to significantly enhance cell proliferation. VEGF and IGF-1 supported ALP activity. Light microscopy showed initial extracellular matrix mineralization after VEGF/IGF-1 supply. Conclusion A combination of more than two growth factors did not support the cellular metabolism level and ALP activity even though the growth factor itself had a positive effect. This is probably caused by interplay of various messengers shared by more growth factor signalling cascades. Cite this article: Bone Joint Res 2020;9(7):412–420.

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