Regulation of Notch Signaling in Drosophila melanogaster: The Role of the Heterogeneous Nuclear Ribonucleoprotein Hrp48 and Deltex

2020 ◽  
pp. 95-105
Author(s):  
Debdeep Dutta ◽  
Mousumi Mutsuddi ◽  
Ashim Mukherjee
Keyword(s):  
Drosophila Melanogaster ◽  
Notch Signaling ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein

scholarly journals Regulation of Notch Signaling by the Heterogeneous Nuclear Ribonucleoprotein Hrp48 and Deltex in Drosophila melanogaster

Genetics ◽  
2017 ◽  
Vol 206 (2) ◽  
pp. 905-918 ◽  
Author(s):  
Debdeep Dutta ◽  
Maimuna Sali Paul ◽  
Ankita Singh ◽  
Mousumi Mutsuddi ◽  
Ashim Mukherjee
Keyword(s):  
Drosophila Melanogaster ◽  
Notch Signaling ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein

Heterogeneous nuclear ribonucleoprotein complexes and proteins in Drosophila melanogaster

1992 ◽  
Vol 12 (2) ◽  
pp. 847-855
Author(s):  
G Raychaudhuri ◽  
S R Haynes ◽  
A L Beyer
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Rna Sequences ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Culture Cells ◽  
Ribonucleoprotein Complexes ◽  
Tissue Culture Cells ◽  
Hnrnp Proteins ◽  
Nuclear Ribonucleoprotein ◽  
Basic Region

Pre-mRNAs cotranscriptionally associate with a small group of proteins to form heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. We have previously identified two genes in Drosophila melanogaster, Hrb98DE and Hrb87F (i.e., genes at 98DE and 87F encoding putative hnRNA binding proteins), which encode five protein species homologous to the mammalian A-B hnRNP proteins. The studies presented herein show that antibodies against the RNP domains of Hrb98DE reacted with 10 to 15 distinct spots of 38 to 40 kDa in the basic region of two-dimensional gels. These nuclear proteins bound single-stranded nucleic acids and were extracted from Drosophila tissue culture cells as 40 to 80S hnRNP complexes in association with 300 to 800 nucleotide fragments of RNA. The peak of poly(A)+ RNA sequences was coincident with the peak of HRB proteins in sucrose gradients, strongly suggesting that the HRB complexes identified are Drosophila hnRNP complexes. The repertoire of HRB proteins did not change significantly during embryogenesis and was similar to that observed in Drosophila tissue culture cells. Analyses with peptide-specific antisera demonstrated that the major proteins in the hnRNP complex were encoded by the two genes previously identified. Although the Drosophila HRB proteins are only approximately 60% identical throughout the RNP domains to the mammalian A-B hnRNP proteins, features of the basic pre-mRNA packaging mechanism appear to be highly conserved between D. melanogaster and mammals.

scholarly journals Role of Notch signaling in establishing the hemilineages of secondary neurons in Drosophila melanogaster

Development ◽  
2009 ◽  
Vol 137 (1) ◽  
pp. 53-61 ◽  
Author(s):  
J. W. Truman ◽  
W. Moats ◽  
J. Altman ◽  
E. C. Marin ◽  
D. W. Williams
Keyword(s):  
Drosophila Melanogaster ◽  
Notch Signaling

scholarly journals Arginine methylation of hnRNPK negatively modulates apoptosis upon DNA damage through local regulation of phosphorylation

2014 ◽  
Vol 42 (15) ◽  
pp. 9908-9924 ◽  
Author(s):  
Jen-Hao Yang ◽  
Yi-Ying Chiou ◽  
Shu-Ling Fu ◽  
I-Yun Shih ◽  
Tsai-Hsuan Weng ◽  
...  
Keyword(s):  
Dna Damage ◽  
Rna Processing ◽  
Tumor Development ◽  
Arginine Methylation ◽  
Wild Type ◽  
Post Translational Modifications ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Damage Response ◽  
Nuclear Ribonucleoprotein

Abstract Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is an RNA/DNA-binding protein involved in chromatin remodeling, RNA processing and the DNA damage response. In addition, increased hnRNPK expression has been associated with tumor development and progression. A variety of post-translational modifications of hnRNPK have been identified and shown to regulate hnRNPK function, including phosphorylation, ubiquitination, sumoylation and methylation. However, the functional significance of hnRNPK arginine methylation remains unclear. In the present study, we demonstrated that the methylation of two essential arginines, Arg296 and Arg299, on hnRNPK inhibited a nearby Ser302 phosphorylation that was mediated through the pro-apoptotic kinase PKCδ. Notably, the engineered U2OS cells carrying an Arg296/Arg299 methylation-defective hnRNPK mutant exhibited increased apoptosis upon DNA damage. While such elevated apoptosis can be diminished through addition with wild-type hnRNPK, we further demonstrated that this increased apoptosis occurred through both intrinsic and extrinsic pathways and was p53 independent, at least in part. Here, we provide the first evidence that the arginine methylation of hnRNPK negatively regulates cell apoptosis through PKCδ-mediated signaling during DNA damage, which is essential for the anti-apoptotic role of hnRNPK in apoptosis and the evasion of apoptosis in cancer cells.

Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer

2015 ◽  
Vol 11 (3) ◽  
pp. 743-752 ◽  
Author(s):  
Paul Dowling ◽  
Damian Pollard ◽  
AnneMarie Larkin ◽  
Michael Henry ◽  
Paula Meleady ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumour Tissue ◽  
Blood Samples ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein

Role of hnRNPA2B1 in lung cancer.

scholarly journals Role of human heterogeneous nuclear ribonucleoprotein C1/C2 in dengue virus replication

2015 ◽  
Vol 12 (1) ◽  
pp. 14 ◽  
Author(s):  
Thanyaporn Dechtawewat ◽  
Pucharee Songprakhon ◽  
Thawornchai Limjindaporn ◽  
Chunya Puttikhunt ◽  
Watchara Kasinrerk ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein
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