Polymorphisms of the ovine prion protein (PrP) Gene in the Pramenka Sheep Breed Population(s) in Bosnia and Herzegovina - Kupreski Strain

A total of 216 local crossbred sheep from 16 scrapie-affected Greek flocks and 210 purebred sheep of the milk breeds Chios and Karagouniko from healthy flocks were analysed for scrapie-linked polymorphisms in the prion protein (PrP) gene. Of the 216 sheep in this case–control study, 96 sheep were clinical cases, 25 subclinical cases (asymptomatic at the moment of euthanasia but positive by histopathology and/or ELISA detecting proteinase-resistant PrP) and 95 healthy controls (negative by all evaluations). Polymorphisms at codons 136, 154 and 171 were determined by denaturing gradient gel electrophoresis, followed by RFLP and sequencing. Scrapie, both clinical and subclinical, was associated with the genotypes ARQ/ARQ (88 of 110 sheep of that genotype), ARQ/TRQ (9 of 13), ARQ/AHQ (15 of 38) and VRQ/VRQ (9 of 17). Histopathological lesions were more severe in the clinical cases. Genotypes ARQ/ARR (26 sheep), ARQ/ARK (seven sheep), AHQ/ARR (one sheep), ARH/ARH (one sheep) and ARR/ARH (three sheep) were detected exclusively in healthy control sheep. In the purebred survey, four genotypes were present in the Chios sheep (ARQ/ARQ, ARQ/TRQ, ARQ/AHQ and ARQ/ARR) and four in the Karagouniko sheep (ARQ/ARQ, ARQ/AHQ, ARQ/ARR and ARQ/ARH).

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Serum Withdrawal-Induced Apoptosis in ZrchI Prion Protein (PrP) Gene-Deficient Neuronal Cell Line Is Suppressed by PrP, Independent of Doppel

Microbiology and Immunology ◽

10.1111/j.1348-0421.2007.tb03920.x ◽

2007 ◽

Vol 51 (4) ◽

pp. 457-466 ◽

Cited By ~ 12

Author(s):

Takuya Nishimura ◽

Akikazu Sakudo ◽

Yoriko Hashiyama ◽

Akiko Yachi ◽

Keiichi Saeki ◽

...

Keyword(s):

Cell Line ◽

Prion Protein ◽

Neuronal Cell ◽

Neuronal Cell Line ◽

Serum Withdrawal ◽

Induced Apoptosis ◽

Prp Gene

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Identification of new allelic variants in the ovine prion protein (PrP) gene

Journal of Animal Breeding and Genetics ◽

10.1046/j.1439-0388.2002.00335.x ◽

2002 ◽

Vol 119 (4) ◽

pp. 201-208 ◽

Cited By ~ 25

Author(s):

T. KUTZER ◽

I. PFEIFFER ◽

B. BRENIG

Keyword(s):

Prion Protein ◽

Allelic Variants ◽

Prp Gene

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Cloning and expression of a prion protein (PrP) gene from Korean bovine (Bos taurus coreanae) and production of rabbit anti-bovine PrP antibody

Biotechnology Letters ◽

10.1007/s10529-008-9768-4 ◽

2008 ◽

Vol 30 (10) ◽

pp. 1705-1711 ◽

Cited By ~ 4

Author(s):

Wooseok Shin ◽

Byungwoo Lee ◽

Sungyoul Hong ◽

Chongsuk Ryou ◽

Moosik Kwon

Keyword(s):

Prion Protein ◽

Bos Taurus ◽

Cloning And Expression ◽

Prp Gene

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The prion protein gene: a role in mouse embryogenesis?

Development ◽

10.1242/dev.115.1.117 ◽

1992 ◽

Vol 115 (1) ◽

pp. 117-122 ◽

Cited By ~ 1

Author(s):

J. Manson ◽

J.D. West ◽

V. Thomson ◽

P. McBride ◽

M.H. Kaufman ◽

...

Keyword(s):

Gene Expression ◽

Nervous System ◽

Prion Protein ◽

In Situ Hybridisation ◽

Neuronal Cell ◽

Normal Function ◽

Cell Populations ◽

Specific Expression ◽

The Brain ◽

Prp Gene

The neural membrane glycoprotein PrP (prion protein) has a key role in the development of scrapie and related neurodegenerative diseases. During pathogenesis, PrP accumulates in and around cells of the brain from which it can be isolated in a disease-specific, protease-resistant form. Although the involvement of PrP in the pathology of these diseases has long been known, the normal function of PrP remains unknown. Previous studies have shown that the PrP gene is expressed tissue specifically in adult animals, the highest levels in the brain, with intermediate levels in heart and lung and low levels in spleen. Prenatally, PrP mRNA has been detected in the brain of rat and hamster just prior to birth. In this study we have examined the expression of the PrP gene during mouse embryonic development by in situ hybridisation and observed dramatic regional and temporal gene expression in the embryo. Transcripts were detected in developing brain and spinal cord by 13.5 days. In addition, PrP gene expression was detected in the peripheral nervous system, in ganglia and nerve trunks of the sympathetic nervous system and neural cell populations of sensory organs. Expression of the PrP gene was not limited to neuronal cells, but was also detected in specific non-neuronal cell populations of the 13.5 and 16.5 day embryos and in extra-embryonic tissues from 6.5 days. This cell-specific expression suggests a pleiotropic role for PrP during development.

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