Cloning and expression of a prion protein (PrP) gene from Korean bovine (Bos taurus coreanae) and production of rabbit anti-bovine PrP antibody

A total of 216 local crossbred sheep from 16 scrapie-affected Greek flocks and 210 purebred sheep of the milk breeds Chios and Karagouniko from healthy flocks were analysed for scrapie-linked polymorphisms in the prion protein (PrP) gene. Of the 216 sheep in this case–control study, 96 sheep were clinical cases, 25 subclinical cases (asymptomatic at the moment of euthanasia but positive by histopathology and/or ELISA detecting proteinase-resistant PrP) and 95 healthy controls (negative by all evaluations). Polymorphisms at codons 136, 154 and 171 were determined by denaturing gradient gel electrophoresis, followed by RFLP and sequencing. Scrapie, both clinical and subclinical, was associated with the genotypes ARQ/ARQ (88 of 110 sheep of that genotype), ARQ/TRQ (9 of 13), ARQ/AHQ (15 of 38) and VRQ/VRQ (9 of 17). Histopathological lesions were more severe in the clinical cases. Genotypes ARQ/ARR (26 sheep), ARQ/ARK (seven sheep), AHQ/ARR (one sheep), ARH/ARH (one sheep) and ARR/ARH (three sheep) were detected exclusively in healthy control sheep. In the purebred survey, four genotypes were present in the Chios sheep (ARQ/ARQ, ARQ/TRQ, ARQ/AHQ and ARQ/ARR) and four in the Karagouniko sheep (ARQ/ARQ, ARQ/AHQ, ARQ/ARR and ARQ/ARH).

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Serum Withdrawal-Induced Apoptosis in ZrchI Prion Protein (PrP) Gene-Deficient Neuronal Cell Line Is Suppressed by PrP, Independent of Doppel

Microbiology and Immunology ◽

10.1111/j.1348-0421.2007.tb03920.x ◽

2007 ◽

Vol 51 (4) ◽

pp. 457-466 ◽

Cited By ~ 12

Author(s):

Takuya Nishimura ◽

Akikazu Sakudo ◽

Yoriko Hashiyama ◽

Akiko Yachi ◽

Keiichi Saeki ◽

...

Keyword(s):

Cell Line ◽

Prion Protein ◽

Neuronal Cell ◽

Neuronal Cell Line ◽

Serum Withdrawal ◽

Induced Apoptosis ◽

Prp Gene

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Identification of new allelic variants in the ovine prion protein (PrP) gene

Journal of Animal Breeding and Genetics ◽

10.1046/j.1439-0388.2002.00335.x ◽

2002 ◽

Vol 119 (4) ◽

pp. 201-208 ◽

Cited By ~ 25

Author(s):

T. KUTZER ◽

I. PFEIFFER ◽

B. BRENIG

Keyword(s):

Prion Protein ◽

Allelic Variants ◽

Prp Gene

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Cloning and expression analysis of a prion protein encoding gene in guppy (Poecilia reticulata)

Journal of Ocean University of China ◽

10.1007/s11802-008-0425-2 ◽

2008 ◽

Vol 7 (4) ◽

pp. 425-431

Author(s):

Suihan Wu ◽

Qiwei Wei ◽

Guanpin Yang ◽

Dengqiang Wang ◽

Guiwei Zou ◽

...

Keyword(s):

Prion Protein ◽

Expression Analysis ◽

Poecilia Reticulata ◽

Cloning And Expression ◽

Protein Encoding ◽

Encoding Gene

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Cloning and expression of caprine KIT gene and associations of polymorphisms with litter size

Animal Production Science ◽

10.1071/an13497 ◽

2016 ◽

Vol 56 (10) ◽

pp. 1579 ◽

Cited By ~ 1

Author(s):

X. P. An ◽

J. X. Hou ◽

T. Y. Gao ◽

B. Y. Cao

Keyword(s):

Amino Acid ◽

Litter Size ◽

Bos Taurus ◽

Homo Sapiens ◽

Ovis Aries ◽

Cloning And Expression ◽

Nucleotide Polymorphisms ◽

Coding Region ◽

Reading Frame ◽

Kit Gene

The full coding region of KIT mRNA was cloned from the caprine ovary. The results showed the caprine KIT cDNA (GenBank accession number KF364483) contained a 2925-bp open reading frame encoding a protein with 974 amino acid residues. BLAST analysis revealed that the caprine KIT protein had high similarity with that of four species: Ovis aries (99%), Bos taurus (99%), Sus scrofa (94%) and Homo sapiens (90%). The KIT mRNA expression pattern showed that KIT mRNA was expressed highly in kidney, ovary, uterus and breast. Two single nucleotide polymorphisms (g.88430T > A and g.120466G > A) in the caprine KIT gene were detected by PCR–restriction fragment length polymorphism (RFLP) and DNA sequencing in 735 goats of Xinong Saanen, Guanzhong and Boer breeds. The g.88430T > A mutation was a missense mutation (Tyr > Asn at position 409 amino acid of KIT). The association study has been done by jointly analysing all data in one analysis. The result showed that individuals with TT and TA genotypes had their litter size increased by 0.11 and 0.09, respectively, compared with those with AA genotype at the g.88430T > A locus for three goat breeds (P < 0.05). Further analysis revealed that combined genotype TTAA was better than the others for litter size in three goat breeds. Therefore, the biochemical and physiological functions, together with the results obtained in our investigation, suggest that the KIT gene could serve as a genetic marker for litter size in goat breeding.

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The prion protein gene: a role in mouse embryogenesis?

Development ◽

10.1242/dev.115.1.117 ◽

1992 ◽

Vol 115 (1) ◽

pp. 117-122 ◽

Cited By ~ 1

Author(s):

J. Manson ◽

J.D. West ◽

V. Thomson ◽

P. McBride ◽

M.H. Kaufman ◽

...

Keyword(s):

Gene Expression ◽

Nervous System ◽

Prion Protein ◽

In Situ Hybridisation ◽

Neuronal Cell ◽

Normal Function ◽

Cell Populations ◽

Specific Expression ◽

The Brain ◽

Prp Gene

The neural membrane glycoprotein PrP (prion protein) has a key role in the development of scrapie and related neurodegenerative diseases. During pathogenesis, PrP accumulates in and around cells of the brain from which it can be isolated in a disease-specific, protease-resistant form. Although the involvement of PrP in the pathology of these diseases has long been known, the normal function of PrP remains unknown. Previous studies have shown that the PrP gene is expressed tissue specifically in adult animals, the highest levels in the brain, with intermediate levels in heart and lung and low levels in spleen. Prenatally, PrP mRNA has been detected in the brain of rat and hamster just prior to birth. In this study we have examined the expression of the PrP gene during mouse embryonic development by in situ hybridisation and observed dramatic regional and temporal gene expression in the embryo. Transcripts were detected in developing brain and spinal cord by 13.5 days. In addition, PrP gene expression was detected in the peripheral nervous system, in ganglia and nerve trunks of the sympathetic nervous system and neural cell populations of sensory organs. Expression of the PrP gene was not limited to neuronal cells, but was also detected in specific non-neuronal cell populations of the 13.5 and 16.5 day embryos and in extra-embryonic tissues from 6.5 days. This cell-specific expression suggests a pleiotropic role for PrP during development.

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