Oral Biofilms and Secondary Caries Formation

2021 ◽  
pp. 19-35
Author(s):  
Eugenio Brambilla ◽  
Andrei Cristian Ionescu
Keyword(s):  
Secondary Caries ◽  
Oral Biofilms

scholarly journals Interfacial degradation of adhesive composite restorations mediated by oral biofilms and mechanical challenge in an extracted tooth model of secondary caries

2017 ◽  
Vol 66 ◽  
pp. 62-70 ◽  
Author(s):  
Carola A. Carrera ◽  
Yuping Li ◽  
Ruoquiong Chen ◽  
Conrado Aparicio ◽  
Alex Fok ◽  
...  
Keyword(s):  
Secondary Caries ◽  
Composite Restorations ◽  
Oral Biofilms

Cultivated and not-yet-cultivated bacteria in oral biofilms

2009 ◽  
Vol 21 (2) ◽  
Author(s):  
Ingar Olsen ◽  
Dorita Preza ◽  
Jørn A. Aas ◽  
Bruce J. Paster
Keyword(s):  
Oral Biofilms

scholarly journals In Vitro Model Systems for Exploring Oral Biofilms: From Single‐Species Populations to Complex Multi‐Species Communities

2021 ◽  
Author(s):  
Ting L. Luo ◽  
Michael E. Vanek ◽  
Carlos Gonzalez‐Cabezas ◽  
Carl F. Marrs ◽  
Betsy Foxman ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Single Species ◽  
Model Systems ◽  
Oral Biofilms ◽  
Vitro Model

scholarly journals Management of oral biofilms by nisin delivery in adhesive microdevices

2021 ◽  
Author(s):  
Stine Egebro Birk ◽  
Mette Dalskov Mosgaard ◽  
Rolf Bech Kjeldsen ◽  
Anja Boisen ◽  
Rikke Louise Meyer ◽  
...  
Keyword(s):  
Oral Biofilms

Influence of biofilm removal from the tooth-restoration interface on the progression of secondary caries lesions: a preliminary in vitro model study

Biofouling ◽  
2021 ◽  
pp. 1-12
Author(s):  
Cácia Signori ◽  
Tamires Timm Maske ◽  
Vitor Henrique Digmayer Romero ◽  
Maximiliano Sérgio Cenci
Keyword(s):  
In Vitro Model ◽  
Secondary Caries ◽  
Model Study ◽  
Biofilm Removal ◽  
Vitro Model ◽  
Caries Lesions

scholarly journals Antibacterial and antibiofilm activities of cinnamon essential oil nanoemulsion against multi-species oral biofilms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yeo-Jin Jeong ◽  
Hee-Eun Kim ◽  
Su-Jin Han ◽  
Jun-Seon Choi
Keyword(s):  
Essential Oil ◽  
Repeated Measures ◽  
Antibacterial Properties ◽  
Three Solutions ◽  
Bacterial Colony ◽  
Biofilm Model ◽  
Oral Biofilms ◽  
Cinnamon Essential Oil ◽  
Cocamidopropyl Betaine ◽  
Bovine Enamel

AbstractCinnamon essential oil (CEO) has antibacterial properties, but its ability to suppress the formation of multi-species oral biofilms has not been fully elucidated. This study evaluated the antibacterial and antibiofilm activities of cinnamon essential oil nanoemulsion (CEON) against oral biofilms formed using a microcosm biofilm model. The biofilms were formed on bovine enamel specimens over a 7-day period, during which all specimens were treated with one of three solutions: 5% CEON (n = 35), 0.5% cocamidopropyl betaine (n = 35), or 0.12% chlorhexidine gluconate (CHX; n = 35). Antibacterial and antibiofilm activities were determined by the red/green ratios (R/G values) of 7-day-old mature biofilms photographed with quantitative light-induced fluorescence-digital, the number of aciduric bacterial colony-forming units (CFUs) within each biofilm, and the absorbance of bacterial suspensions. One-way and repeated-measures analysis of variance were performed to compare differences among the three solutions. R/G values were lowest in the 0.12% CHX group, but not significantly differ from the 5% CEON group. The number of CFUs and absorbance were lowest in the 5% CEON group. This study showed that nanoemulsified CEO inhibited the maturation of multi-species oral biofilms and the growth of oral microorganisms in biofilms, including aciduric bacteria that cause dental caries.

scholarly journals How to intervene in the caries process in adults: proximal and secondary caries? An EFCD-ORCA-DGZ expert Delphi consensus statement

2020 ◽  
Vol 24 (9) ◽  
pp. 3315-3321 ◽  
Author(s):  
Falk Schwendicke ◽  
Christian H Splieth ◽  
Peter Bottenberg ◽  
Lorenzo Breschi ◽  
Guglielmo Campus ◽  
...  
Keyword(s):  
Consensus Statement ◽  
Delphi Consensus ◽  
Secondary Caries

scholarly journals Acid tolerance in early colonizers of oral biofilms

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Gabriella Boisen ◽  
Julia R. Davies ◽  
Jessica Neilands
Keyword(s):  
Acid Tolerance ◽  
Low Ph ◽  
Salivary Proteins ◽  
Confocal Scanning Laser Microscopy ◽  
Planktonic Cells ◽  
Tolerance Development ◽  
Salivary Pellicle ◽  
Oral Biofilms ◽  
Acid Tolerant

Abstract Background In caries, low pH drives selection and enrichment of acidogenic and aciduric bacteria in oral biofilms, and development of acid tolerance in early colonizers is thought to play a key role in this shift. Since previous studies have focussed on planktonic cells, the effect of biofilm growth as well as the role of a salivary pellicle on this process is largely unknown. We explored acid tolerance and acid tolerance response (ATR) induction in biofilm cells of both clinical and laboratory strains of three oral streptococcal species (Streptococcus gordonii, Streptococcus oralis and Streptococcus mutans) as well as two oral species of Actinomyces (A. naeslundii and A. odontolyticus) and examined the role of salivary proteins in acid tolerance development. Methods Biofilms were formed on surfaces in Ibidi® mini flow cells with or without a coating of salivary proteins and acid tolerance assessed by exposing them to a challenge known to kill non-acid tolerant cells (pH 3.5 for 30 min) followed by staining with LIVE/DEAD BacLight and confocal scanning laser microscopy. The ability to induce an ATR was assessed by exposing the biofilms to an adaptation pH (pH 5.5) for 2 hours prior to the low pH challenge. Results Biofilm formation significantly increased acid tolerance in all the clinical streptococcal strains (P < 0.05) whereas the laboratory strains varied in their response. In biofilms, S. oralis was much more acid tolerant than S. gordonii or S. mutans. A. naeslundii showed a significant increase in acid tolerance in biofilms compared to planktonic cells (P < 0.001) which was not seen for A. odontolyticus. All strains except S. oralis induced an ATR after pre-exposure to pH 5.5 (P < 0.05). The presence of a salivary pellicle enhanced both acid tolerance development and ATR induction in S. gordonii biofilms (P < 0.05) but did not affect the other bacteria to the same extent. Conclusions These findings suggest that factors such as surface contact, the presence of a salivary pellicle and sensing of environmental pH can contribute to the development of high levels of acid tolerance amongst early colonizers in oral biofilms which may be important in the initiation of caries.

scholarly journals The Role of Exopolysaccharides in Oral Biofilms

2019 ◽  
Vol 98 (7) ◽  
pp. 739-745 ◽  
Author(s):  
C. Cugini ◽  
M. Shanmugam ◽  
N. Landge ◽  
N. Ramasubbu
Keyword(s):  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Super Resolution ◽  
Extracellular Proteins ◽  
Extracellular Polysaccharides ◽  
Oral Biofilms ◽  
The Matrix ◽  
Oral Microbes ◽  
Biofilm Development

The oral cavity contains a rich consortium of exopolysaccharide-producing microbes. These extracellular polysaccharides comprise a major component of the oral biofilm. Together with extracellular proteins, DNA, and lipids, they form the biofilm matrix, which contributes to bacterial colonization, biofilm formation and maintenance, and pathogenesis. While a number of oral microbes have been studied in detail with regard to biofilm formation and pathogenesis, the exopolysaccharides have been well characterized for only select organisms, namely Streptococcus mutans and Aggregatibacter actinomycetemcomitans. Studies on the exopolysaccharides of other oral organisms, however, are in their infancy. In this review, we present the current research on exopolysaccharides of oral microbes regarding their biosynthesis, regulation, contributions to biofilm formation and stability of the matrix, and immune evasion. In addition, insight into the role of exopolysaccharides in biofilms is highlighted through the evaluation of emerging techniques such as pH probing of biofilm colonies, solid-state nuclear magnetic resonance for macromolecular interactions within biofilms, and super-resolution microscopy analysis of biofilm development. Finally, exopolysaccharide as a potential nutrient source for species within a biofilm is discussed.

scholarly journals Novel Protein-Repellent and Antibacterial Resins and Cements to Inhibit Lesions and Protect Teeth

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Li Cao ◽  
Junling Wu ◽  
Qiang Zhang ◽  
Bashayer Baras ◽  
Ghalia Bhadila ◽  
...  
Keyword(s):  
Orthodontic Treatment ◽  
The Novel ◽  
Dental Resin ◽  
Biofilm Growth ◽  
New Class ◽  
Dental Resins ◽  
Wide Range ◽  
Oral Biofilms ◽  
One Year

Orthodontic treatment is increasingly popular as people worldwide seek esthetics and better quality of life. In orthodontic treatment, complex appliances and retainers are placed in the patients’ mouths for at least one year, which often lead to biofilm plaque accumulation. This in turn increases the caries-inducing bacteria, decreases the pH of the retained plaque on an enamel surface, and causes white spot lesions (WSLs) in enamel. This article reviews the cutting-edge research on a new class of bioactive and therapeutic dental resins, cements, and adhesives that can inhibit biofilms and protect tooth structures. The novel approaches include the use of protein-repellent and anticaries polymeric dental cements containing 2-methacryloyloxyethyl phosphorylcholine (MPC) and dimethylaminododecyl methacrylate (DMAHDM); multifunctional resins that can inhibit enamel demineralization; protein-repellent and self-etching adhesives to greatly reduce oral biofilm growth; and novel polymethyl methacrylate resins to suppress oral biofilms and acid production. These new materials could reduce biofilm attachment, raise local biofilm pH, and facilitate the remineralization to protect the teeth. This novel class of dental resin with dual benefits of antibacterial and protein-repellent capabilities has the potential for a wide range of dental and biomedical applications to inhibit bacterial infection and protect the tissues.

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