Molecular Adaptation to High Pressure: Proteins in Deep-Sea Animals

2021 ◽  
pp. 151-172
Author(s):  
Alister Macdonald
Keyword(s):  
High Pressure ◽  
Deep Sea ◽  
Molecular Adaptation

A Study of the Sealing Performance of a New High-Pressure Cone Valve for Deep-Sea Gas-Tight Water Samplers

2010 ◽  
Vol 132 (4) ◽  
Author(s):  
Shi-Jun Wu ◽  
Can-Jun Yang ◽  
Ying Chen ◽  
Yan-Qing Xie
Keyword(s):  
Finite Element Analysis ◽  
High Pressure ◽  
Deep Sea ◽  
Hydrothermal Fluid ◽  
Contact Model ◽  
Element Analysis ◽  
Sea Trial ◽  
Sealing Performance ◽  
Pressure Cone ◽  
Gas Tight

The cone valve plays an important role in high-pressure sealing applications. In this paper, a new high-pressure cone valve, based on the titanium alloy poppet-to-polyetheretherketone seat sealing structure, is proposed for deep-sea gas-tight water samplers. In order to study the sealing performance of the new valve, both the conforming poppet-seat contact model and the nonconforming poppet-seat contact model were evaluated. Finite element analysis based on the two models was performed and validated by experiments. The results indicate that the nonconforming poppet-seat contact model has a better sealing performance than the conforming poppet-seat contact model. The new cone valve also was applied in a gas-tight hydrothermal fluid sampler and successfully tested in a sea trial during the KNOX18RR cruise from 9 July to 12 August 2008.

scholarly journals FabF Is Required for Piezoregulation ofcis-Vaccenic Acid Levels and Piezophilic Growth of the Deep-Sea Bacterium Photobacterium profundum Strain SS9

2000 ◽  
Vol 182 (5) ◽  
pp. 1264-1271 ◽  
Author(s):  
Eric E. Allen ◽  
Douglas H. Bartlett
Keyword(s):  
High Pressure ◽  
Hydrostatic Pressure ◽  
Deep Sea ◽  
Unsaturated Fatty Acids ◽  
Acyl Carrier Protein ◽  
Vaccenic Acid ◽  
Bacterial Species ◽  
Elevated Pressure ◽  
Wild Type ◽  
Photobacterium Profundum

ABSTRACT To more fully explore the role of unsaturated fatty acids in high-pressure, low-temperature growth, the fabF gene from the psychrotolerant, piezophilic deep-sea bacteriumPhotobacterium profundum strain SS9 was characterized and its role and regulation were examined. An SS9 strain harboring a disruption in the fabF gene (strain EA40) displayed growth impairment at elevated hydrostatic pressure concomitant with diminishedcis-vaccenic acid (18:1) production. However, growth ability at elevated pressure could be restored to wild-type levels by the addition of exogenous 18:1 to the growth medium. Transcript analysis did not indicate that the SS9 fabF gene is transcriptionally regulated, suggesting that the elevated 18:1 levels produced in response to pressure increase result from posttranscriptional changes. Unlike many pressure-adapted bacterial species such as SS9, the mesophile Escherichia coli did not regulate its fatty acid composition in an adaptive manner in response to changes in hydrostatic pressure. Moreover, an E. coli fabF strain was as susceptible to elevated pressure as wild-type cells. It is proposed that the SS9 fabF product, β-ketoacyl–acyl carrier protein synthase II has evolved novel pressure-responsive characteristics which facilitate SS9 growth at high pressure.

Review of Deep-Ocean High-Pressure Simulation Systems

2020 ◽  
Vol 54 (3) ◽  
pp. 68-84
Author(s):  
Wentao Song ◽  
Weicheng Cui
Keyword(s):  
High Pressure ◽  
Coming Out ◽  
Deep Sea ◽  
Mathematical Theory ◽  
Deep Ocean ◽  
Cold Isostatic Pressing ◽  
Future Design ◽  
Stress Coefficient ◽  
History Of ◽  
Simulation Systems

AbstractDeep-sea technology and equipment are required to explore the oceans and utilize ocean resources in the 21st century. Deep-ocean simulation systems (DOSs) play an essential role in the development of deep-sea equipment. This paper gives a detailed overview of deep-ocean high-pressure simulation systems (DOHPSs) worldwide. First, the history of DOS is introduced, and then the primary available equipment, particularly coming out of China, is described. Next, the new concept of the cold isostatic pressing (CIP) chamber and its technology and equipment are reviewed. Then, the basic mathematical theory for the design of pressure chambers is introduced to illustrate the limitations of the traditional monobloc chamber. To easily understand the pre-stressed wire-wound (PSWW) design, the pre-stress coefficient is introduced in theoretical analysis. Some valuable researches of PSWW are presented. Finally, the sealing design of DOS, especially tooth-locked quick-actuating closures (TLQAC), is discussed. The paper aims to inspire readers to develop innovative ideas about the future design of DOS.

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