An Ancient Medicinal Plant at the Crossroads of Modern Horticulture and Genetics: Genetic Resources and Biotechnology of Sea Buckthorn (Hippophae L., Elaeagnaceae)

2016 ◽  
pp. 415-446 ◽  
Author(s):  
Igor V. Bartish
Keyword(s):  
Medicinal Plant ◽  
Genetic Resources ◽  
Sea Buckthorn

Ex-situ conservation of medicinal plant genetic resources managed by national agrobiodiversity center of Korea

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
JS Sung ◽  
CW Jeong ◽  
YY Lee ◽  
HS Lee ◽  
YA Jeon ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Resources ◽  
Ex Situ Conservation ◽  
Plant Genetic Resources ◽  
Ex Situ

scholarly journals Comparative Characteristics of Determination of Antioxidant Activity of Sea Buckthorn Fruits by Various Methods

2019 ◽  
Vol 8 (4) ◽  
pp. 48-52
Author(s):  
O. V. Trineeva
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Plant Material ◽  
Natural Antioxidants ◽  
Sea Buckthorn ◽  
In Vitro Tests ◽  
Medicinal Plant Material

Introduction. Recently, much attention has been paid to the primary assessment of the pharmacological effect of various drugs using in vivo and in vitro tests. It is known that such a medicinal plant as sea buckthorn, in its phytochemical composition is rich in natural antioxidants: carotenoids, tocopherols, flavonoids, ascorbic acid, etc. In some publications there is information about the antioxidant activity of sea buckthorn and fatty oil based on them. However, information on the comparative characteristics of the use of various methods for determining the antioxidant activity of this type of medicinal plant material and the results obtained are not found in the scientific literature.Aim. The aim of this work was a comparative determination of the antioxidant activity of medicinal plant material of buckthorn fruits of various species of buckthorn.Materials and methods. The total antioxidant activity of water and water-alcohol extracts from the fruits of sea buckthorn fruits was determined using various techniques recommended in the literature. The antioxidant activity of the extracts was determined by permanganometric titration, in vitro inhibition of adrenaline autooxidation, and also in a biological model, Parametium caudatum cell culture.Results and discussion. The effect of the extractant polarity on the value of antioxidant activity was studied. It was found that the highest content of antioxidants in the extraction is observed when using 96 % ethanol as an extractant.Conclusion. Using three methods, the prospects of using sea buckthorn fruits and preparations based on them as a source of antioxidants are shown. 

scholarly journals Application of Luminescent Microscopy in Analysis of Anatomic-diagnostic Signs of Fruits of Sea Buckthorn

2020 ◽  
Vol 9 (1) ◽  
pp. 40-45
Author(s):  
O. V. Trineeva ◽  
A. A. Gudkova ◽  
M. A. Rudaya
Keyword(s):  
Medicinal Plant ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Sea Buckthorn ◽  
Biologically Active ◽  
Plant Materials ◽  
Fatty Oil ◽  
Luminescent Analysis ◽  
Diagnostic Signs ◽  
Promising Source

Introduction. Sea buckthorn (Hippophae rhamnoides), the family Eleagnaceae – a promising source of biologically active substances, the traditional raw materials of which are fruits. The existing regulatory documentation (ND) lacks such an indicator of the authenticity and good quality of the fruit as «microscopy». Despite the available data on the anatomy and histology of the fruits of this type of medicinal plant material (MPM), the scientific literature does not describe the luminescence features of the tissues of sea buckthorn fruit. It is known that the luminescent features of tissues make it possible to identify the localization of biologically active structures, and also, in some cases, to conduct selective diagnosis of MPM.Aim. The aim of this work was to study the characteristics of the luminescence of the tissues of dried crushed fruits of sea buckthorn, and also to optimize the conditions for analysis.Materials and methods. The object of the study was the dried, crushed fruits of sea buckthorn of various species. The study of microdiagnostic signs was carried out according to the State Pharmacopoeia of the XIV RF GPA.1.5.3.0003.15 «Technique of microscopic and microchemical studies of medicinal plant materials and herbal medicines». A stereomicroscopic study was carried out on a Biomed-6 microscope. The luminescence of sea buckthorn fruit tissues was examined using a luminescent microscope of the Micromed-3 Lum brand.Results and discussion. The most pronounced yellow glow is observed for the tissue of the pulp and epidermis of the fetus, which is associated with the highest content of fatty oil in these structures. Groups of phenolic compounds contained along with oil give a greenish tint to objects, while condensed tannins give a brownish tint. The plates that make up the corymbose hairs do not exhibit bright luminescence; a weak glow is characteristic of the joints of the hair platelets. The walls of stellate hairs have a faint greenish glow. Fragments of the actual fruit («sac») are brownish in color, without their own luminescence, as well as the seed peel. The embryo has its own greenish luminescence due to the presence of both a fatty oil and a complex of storage substances.Conclusion. The luminescent analysis of sea buckthorn fruits was carried out for the first time. The choice of a method for preparing the studied MPM for microscopic examination is experimentally substantiated. The luminescent analysis revealed the peculiarities of the glow of the tissues of the fruits of sea buckthorn. The main microdiagnostic signs of crushed dried sea buckthorn fruits and their biometric characteristics are specified. The analysis of the obtained data will allow us to further develop the section «Microscopic signs» for inclusion in the PA project on MPM, widely cultivated and used by the domestic pharmaceutical industry for the production of herbal medicines.

Creating a collection of the sea buckthorn (Hippophae rhamnoides L.) gene pool

2020 ◽  
pp. 65-76
Author(s):  
V.V. Moskalets ◽  
T.Z. Moskalets ◽  
I.V. Grynyk ◽  
O.B. Ovezmyradova ◽  
O.M. Nevmerzhytska
Keyword(s):  
Genetic Resources ◽  
Gene Pool ◽  
Plant Genetic Resources ◽  
Sea Buckthorn ◽  
Forest Steppe ◽  
Expert Evaluation ◽  
Northern Forest ◽  
Hippophaë Rhamnoides ◽  
Morphobiological Characteristics ◽  
Genetic Profiles

Аim. To create a working collection of the sea buckthorn gene pool for priority breeding trends. Results and Discussion. Scientific and practical results of building up and studying the working collection of the buckthorn gene pool of the Institute of Horticulture of NAAS are presented. Aspects of formation of a working collection and their comparative identification by genetic profiles are analyzed. Morphobiological characteristics and economically valuable features of sea buckthorn plants are summarized. Conclusions. Targeted introduction and studies of the adaptive and productive potentials of sea buckthorn in the experimental fields of the Institute of Horticulture (northern forest-steppe of Ukraine) in 2017-2019 allowed us to transfer the most promising genotypes to the National Center for Plant Genetic Resources of Ukraine (NCPGRU) of the Рlant Production Institute named after VYa Yuriev of NAAS of Ukraine, where, after expert evaluation, they were assigned catalog numbers and included in a list of plants of the genetic bank of Ukraine: F 1-15-1 or Nosivchanka (UA3700073), F 1-15-8S or Mitsna (UA3700079), male form 1-15 -6Ch or Aboryhеn 6/11 (UA3700080), F 1-15-9 or Karotynna (UA3700082), F 1-15-3 or Pamiatka (UA3700076), F 1-15-8V or Soniachne Siayvo (UA3700075), 1-15-11 or Lymonna (UA3700072), F 2-15-73 or Morkviana (UA3700077), F 1-15-5 or Adaptyvna (UA3700078), F 1-15-8B or Osoblyva (UA3700083), F 1-15-6 or Apelsynova (UA3700084), F 6А/11 (UA3700081), and F 1-15-5а or Sribnolysta 5a (UA3700074).

Environment Pressure and Countermeasures of the Development of the Natural Medicine Source Plants

2012 ◽  
Vol 610-613 ◽  
pp. 3319-3322
Author(s):  
Guo Sheng Chen
Keyword(s):  
Heavy Metal ◽  
Medicinal Plants ◽  
Medicinal Plant ◽  
Genetic Resources ◽  
Social Environment ◽  
Pesticide Residues ◽  
Raw Materials ◽  
Harvest Time ◽  
Plant Resources ◽  
Natural Medicine

due to the disordering and robbing exploration, the development of natural medicinal plant resources is facing severe habitat and social environment pressure that may touch the law in the process of getting raw materials from plants. Based on the above problems, to develop and enhance the distribution census about the new medicinal plant resources is a good way to cope with the resource shortages. In addition, for the validated medicinal plants, the scaled and standardized production is also an effective mean. Moreover, we should also have the whole dynamic management about the standardized production. That is to say, we should choose the excellent genetic resources, consider the regional characteristics, complete the gardens environment evaluation, grasp the best harvest time and strengthen the detection of the pesticide residues and heavy metal.

Sea Buckthorn: A Multipurpose Medicinal Plant from Upper Himalayas

2019 ◽  
pp. 399-426
Author(s):  
Ashish Yadav ◽  
Tsering Stobdan ◽  
O. P. Chauhan ◽  
S. K. Dwivedi ◽  
O. P. Chaurasia
Keyword(s):  
Medicinal Plant ◽  
Sea Buckthorn

scholarly journals DETERMINATION OF SIMPLE SUGARS IN MEDICINAL PLANT MATERIALS BY THE METHOD OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY (FOR EXAMPLE, THE FRUITS OF SEA BUCKTHORN L. AND NETTLE LEAVES L.)

2020 ◽  
pp. 215-222
Author(s):  
Ol'ga Valer'yevna Trineeva ◽  
Aleksey Ivanovich Slivkin
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Sea Buckthorn ◽  
Standard Samples ◽  
Plant Materials ◽  
Layer Chromatography

An analysis of the literature over the past 20 years has shown that when controlling the quality of drugs containing monosaccharides, as well as studying the composition of simple sugars in polysaccharide complexes of medicinal plants and not only, preference is given to physicochemical methods, as the most express, sensitive and informative. No means have been found in the scientific literature to identify and quantify simultaneously various monosaccharides by high performance thin layer chromatography (HPTLC). An economical and rapid method has been developed for the identification and quantitative determination of simple reducing sugars (by the example of glucose, rhamnose and xylose) by the HPTLC method. The optimal conditions for their chromatography in a thin layer of sorbent with a quantitative interpretation of HPTLC data on a personal computer were experimentally selected and theoretically substantiated. In a detailed study of the influence of the polarity of the system on the value of Rf, the intervals of values of the polarity of the eluent were chosen, in which these dependences become linear. Using the proposed dependencies, you can select different systems for the separation of monosaccharides in a thin layer of sorbent, so that the value of Rf fit into the optimal values. The proposed method was tested on medicinal plant raw materials of nettle dioica and sea buckthorn fruits of various conservation methods. Zones of simple sugars of characteristic color were found on the chromatograms of extracts from the studied raw materials, among which glucose, xylose and rhamnose were identified by the characteristic value of Rf values in comparison with reliable standard samples. The technique can be used in quality control of substances, single-component and complex preparations, plant objects, dietary supplements, premixes and products of the food industry.

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