An Integrative Approach for the Functional Analysis of Metagenomic Studies

An integrative analysis identifying transcriptional features and key genes involved in COVID-19

Epigenomics ◽

10.2217/epi-2020-0168 ◽

2020 ◽

Vol 12 (22) ◽

pp. 1969-1981

Author(s):

Guoping Li ◽

Junyi Wang ◽

Xiang He ◽

Lei Zhang ◽

Qin Ran ◽

...

Keyword(s):

Functional Analysis ◽

Immune Cells ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Integrative Approach ◽

Molecular Features ◽

Infected Cells ◽

Key Genes ◽

Upregulated Genes ◽

Core Genes

Aim: To elucidate the transcriptional characteristics of COVID-19. Materials & methods: We utilized an integrative approach to comprehensively analyze the transcriptional features of both COVID-19 patients and SARS-CoV-2 infected cells. Results: Widespread infiltration of immune cells was observed. We identified 233 genes that were codifferentially expressed in both bronchoalveolar lavage fluid and lung samples of COVID-19 patients. Functional analysis suggested upregulated genes were related to immune response such as neutrophil activation and antivirus response, while downregulated genes were associated with cell adhesion. Finally, we identified LCN2, STAT1 and UBE2L6 as core genes during SARS-CoV-2 infection. Conclusion: The identification of core genes involved in COVID-19 can provide us with more insights into the molecular features of COVID-19.

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Perceptions of a Collaborative Professional Learning Program: Seeing the “Bigger Picture”

Perspectives of the ASHA Special Interest Groups ◽

10.1044/2019_persp-19-00041 ◽

2020 ◽

Vol 5 (1) ◽

pp. 290-303

Author(s):

P. Charlie Buckley ◽

Kimberly A. Murza ◽

Tami Cassel

Keyword(s):

Data Analysis ◽

Professional Learning ◽

Social Communication ◽

Special Educators ◽

Service Providers ◽

Integrative Approach ◽

Theory Approach ◽

Learning Program ◽

Speech Language Pathologists ◽

Related Service

Purpose The purpose of this study was to explore the perceptions of special education practitioners (i.e., speech-language pathologists, special educators, para-educators, and other related service providers) on their role as communication partners after participation in the Social Communication and Engagement Triad (Buckley et al., 2015 ) yearlong professional learning program. Method A qualitative approach using interviews and purposeful sampling was used. A total of 22 participants who completed participation in either Year 1 or Year 2 of the program were interviewed. Participants were speech-language pathologists, special educators, para-educators, and other related service providers. Using a grounded theory approach (Glaser & Strauss, 1967 ) to data analysis, open, axial, and selective coding procedures were followed. Results Three themes emerged from the data analysis and included engagement as the goal, role as a communication partner, and importance of collaboration. Conclusions Findings supported the notion that educators see the value of an integrative approach to service delivery, supporting students' social communication and engagement across the school day but also recognizing the challenges they face in making this a reality.

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Clinical Issues: What’s in a Word? A Functional Analysis of Word Learning

Perspectives on Language Learning and Education ◽

10.1044/lle12.3.4 ◽

2005 ◽

Vol 12 (3) ◽

pp. 4-8 ◽

Cited By ~ 7

Author(s):

Prahlad Gupta

Keyword(s):

Functional Analysis ◽

Word Learning ◽

Clinical Issues

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Review for "High‐throughput single‐cell sequencing of paired TCRα and TCRβ genes for the direct expression‐cloning and functional analysis of murine T cell receptors"

10.1002/eji.201848030/v1/review1 ◽

2019 ◽

Keyword(s):

Functional Analysis ◽

T Cell ◽

Single Cell ◽

High Throughput ◽

T Cell Receptors ◽

Expression Cloning ◽

Cell Receptors ◽

Single Cell Sequencing

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Case Formulation and Design of Behavioral Treatment Programs

European Journal of Psychological Assessment ◽

10.1027//1015-5759.19.3.164 ◽

2003 ◽

Vol 19 (3) ◽

pp. 164-174 ◽

Cited By ~ 27

Author(s):

Stephen N. Haynes ◽

Andrew E. Williams

Keyword(s):

Functional Analysis ◽

Behavior Problems ◽

Behavioral Treatment ◽

Clinical Case ◽

Relative Magnitude ◽

Mechanisms Of Action ◽

Treatment Programs ◽

Case Formulation ◽

Functional Relations ◽

Causal Variables

Summary: We review the rationale for behavioral clinical case formulations and emphasize the role of the functional analysis in the design of individualized treatments. Standardized treatments may not be optimally effective for clients who have multiple behavior problems. These problems can affect each other in complex ways and each behavior problem can be influenced by multiple, interacting causal variables. The mechanisms of action of standardized treatments may not always address the most important causal variables for a client's behavior problems. The functional analysis integrates judgments about the client's behavior problems, important causal variables, and functional relations among variables. The functional analysis aids treatment decisions by helping the clinician estimate the relative magnitude of effect of each causal variable on the client's behavior problems, so that the most effective treatments can be selected. The parameters of, and issues associated with, a functional analysis and Functional Analytic Clinical Case Models (FACCM) are illustrated with a clinical case. The task of selecting the best treatment for a client is complicated because treatments differ in their level of specificity and have unequally weighted mechanisms of action. Further, a treatment's mechanism of action is often unknown.

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