scholarly journals Repeatability and Reproducibility of In Vivo Cone Density Measurements in the Adult Zebrafish Retina

2018 ◽  
pp. 151-156 ◽  
Author(s):  
Alison Huckenpahler ◽  
Melissa Wilk ◽  
Brian Link ◽  
Joseph Carroll ◽  
Ross Collery
Keyword(s):  
Adult Zebrafish ◽  
Density Measurements ◽  
Repeatability And Reproducibility ◽  
Cone Density

Combretum lanceolatum extract reverses anxiety and seizure behavior in adult zebrafish through GABAergic neurotransmission: an in vivo and in silico study

2021 ◽  
pp. 1-14
Author(s):  
Antonio Wlisses da Silva ◽  
Maria Kueirislene A. Ferreira ◽  
Lucas Ramos Pereira ◽  
Emanuela L. Rebouças ◽  
Marnielle Rodrigues Coutinho ◽  
...  
Keyword(s):  
In Silico ◽  
Adult Zebrafish ◽  
Gabaergic Neurotransmission ◽  
In Silico Study ◽  
Combretum Lanceolatum

scholarly journals The Reliability of Cone Density Measurements in the Presence of Rods

2018 ◽  
Vol 7 (3) ◽  
pp. 21 ◽  
Author(s):  
Jessica I. W. Morgan ◽  
Grace K. Vergilio ◽  
Jessica Hsu ◽  
Alfredo Dubra ◽  
Robert F. Cooper
Keyword(s):  
Density Measurements ◽  
Cone Density

scholarly journals Modeling Human Cardiac Arrhythmias: Insights from Zebrafish

2022 ◽  
Vol 9 (1) ◽  
pp. 13
Author(s):  
Sébastien Gauvrit ◽  
Jaclyn Bossaer ◽  
Joyce Lee ◽  
Michelle M. Collins
Keyword(s):  
Public Health ◽  
Cardiac Arrhythmia ◽  
Cardiac Arrhythmias ◽  
Heart Rhythm ◽  
Therapeutic Strategies ◽  
Adult Zebrafish ◽  
Disease Mechanisms ◽  
New Models ◽  
Public Health Challenges

Cardiac arrhythmia, or irregular heart rhythm, is associated with morbidity and mortality and is described as one of the most important future public health challenges. Therefore, developing new models of cardiac arrhythmia is critical for understanding disease mechanisms, determining genetic underpinnings, and developing new therapeutic strategies. In the last few decades, the zebrafish has emerged as an attractive model to reproduce in vivo human cardiac pathologies, including arrhythmias. Here, we highlight the contribution of zebrafish to the field and discuss the available cardiac arrhythmia models. Further, we outline techniques to assess potential heart rhythm defects in larval and adult zebrafish. As genetic tools in zebrafish continue to bloom, this model will be crucial for functional genomics studies and to develop personalized anti-arrhythmic therapies.

scholarly journals Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5840
Author(s):  
Hiroko Nakayama ◽  
Kanae Hata ◽  
Izumi Matsuoka ◽  
Liqing Zang ◽  
Youngil Kim ◽  
...  
Keyword(s):  
Natural Products ◽  
Adverse Effects ◽  
Lipid Accumulation ◽  
Juvenile Fish ◽  
Visceral Obesity ◽  
Adult Zebrafish ◽  
Obesity Epidemic ◽  
In Vivo Testing ◽  
Tea Extract

(1) Background: The obesity epidemic has been drastically progressing in both children and adults worldwide. Pharmacotherapy is considered necessary for its treatment. However, many anti-obesity drugs have been withdrawn from the market due to their adverse effects. Instead, natural products (NPs) have been studied as a source for drug discovery for obesity, with the goal of limiting the adverse effects. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. However, the evidence for zebrafish as an anti-obesity drug screening model are still limited. (2) Methods: We performed anti-adipogenic testing using the juvenile zebrafish obesogenic test (ZOT) and mouse 3T3-L1 preadipocytes using the focused NP library containing 38 NPs and compared their results. (3) Results: Seven and eleven NPs reduced lipid accumulation in zebrafish visceral fat tissues and mouse adipocytes, respectively. Of these, five NPs suppressed lipid accumulation in both zebrafish and 3T3-L1 adipocytes. We confirmed that these five NPs (globin-digested peptides, green tea extract, red pepper extract, nobiletin, and Moringa leaf powder) exerted anti-obesity effects in diet-induced obese adult zebrafish. (4) Conclusions: ZOT using juvenile fish can be a high-throughput alternative to ZOT using adult zebrafish and can be applied for in vivo screening to discover novel therapeutics for visceral obesity and potentially also other disorders.

scholarly journals Reliability and Repeatability of Cone Density Measurements in Patients With Stargardt Disease and RPGR-Associated Retinopathy

2017 ◽  
Vol 58 (9) ◽  
pp. 3608 ◽  
Author(s):  
Preena Tanna ◽  
Melissa Kasilian ◽  
Rupert Strauss ◽  
James Tee ◽  
Angelos Kalitzeos ◽  
...  
Keyword(s):  
Stargardt Disease ◽  
Density Measurements ◽  
Cone Density

scholarly journals Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging

Sensors ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 4094 ◽  
Author(s):  
Sunmi Yeo ◽  
Changhan Yoon ◽  
Ching-Ling Lien ◽  
Tai-Kyong Song ◽  
K. Kirk Shung
Keyword(s):  
High Frequency ◽  
Doppler Imaging ◽  
Adult Zebrafish ◽  
High Frequency Ultrasound ◽  
In Vivo Experiments ◽  
Longitudinal Measurements ◽  
Array Transducer ◽  
Spectral Doppler ◽  
Frequency Ultrasound

This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A 30-MHz high-frequency ultrasound array transducer was used to acquire backscattered echo signal for spectral Doppler and Nakagami imaging. The performances of both methods were validated with flow and tissue-mimicking phantom experiments. For in vivo experiments, both spectral Doppler and Nakagami imaging were simultaneously obtained from adult zebrafish with amputated hearts. Longitudinal measurements were performed for five zebrafish. From the experiments, the E/A ratio measured using spectral Doppler imaging increased at 3 days post-amputation (3 dpa) and then decreased to the value before amputation, which were consistent with previous studies. Similar results were obtained from the Nakagami imaging where the Nakagami parameter value increased at 3 dpa and decreased to its original value. These results suggested that the Nakagami and spectral Doppler imaging would be useful techniques in monitoring the regeneration of heart or tissues.

scholarly journals Interocular symmetry, intraobserver repeatability, and interobserver reliability of cone density measurements in the 13-lined ground squirrel

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0223110 ◽  
Author(s):  
Benjamin S. Sajdak ◽  
Alexander E. Salmon ◽  
Rachel E. Linderman ◽  
Jenna A. Cava ◽  
Heather Heitkotter ◽  
...  
Keyword(s):  
Ground Squirrel ◽  
Interobserver Reliability ◽  
Density Measurements ◽  
Cone Density
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