Analysis of Skeletal Muscle System Loads for the Most Optimal Positions During Lifting in Different Load Distances

The amazing behaviour and adaptation capacity of the skeletal muscle system call the attention of several scientists, including us. Thus, we have the pleasure to announce that it was born in the city of Coimbra, Portugal, the Portuguese Society of Myology (SPMyo), a society which the main aim is to spread skeletal muscle knowledge in the areas of muscle physiology, physiopathology and pharmacology in a multidisciplinary organization that reflects the different areas of the skeletal muscle study. The commitment of SPMyo is the progress of skeletal muscle awareness as well as its investigation, encouraging interdisciplinary, national and international collaboration. These goals will be achieved by organization of scientific meetings, formation courses, publications of books and other scientific and didactic materials. It is also a goal of the SPMyo to organize public events allowing general public to understand skeletal muscle system.

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Redox Signaling and the Cardiovascular and Skeletal Muscle System

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/849095 ◽

2015 ◽

Vol 2015 ◽

pp. 1-2 ◽

Cited By ~ 3

Author(s):

Aldrin V. Gomes ◽

Namakkal S. Rajasekaran ◽

Xinchun Pi

Keyword(s):

Skeletal Muscle ◽

Redox Signaling ◽

Muscle System

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Electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues

AJP Cell Physiology ◽

10.1152/ajpcell.00049.2021 ◽

2021 ◽

Author(s):

Christine T. Nguyen ◽

Majid Ebrahimi ◽

Penney M. Gilbert ◽

Bryan Andrew Stewart

Keyword(s):

Skeletal Muscle ◽

Muscle Cell ◽

Muscle Tissue ◽

Three Dimensional ◽

Dystrophin Gene ◽

Muscle System ◽

Electrophysiological Analysis ◽

Cytoskeletal Network ◽

Muscle Tissues

Recently, methods for creating three-dimensional (3D) human skeletal muscle tissues from myogenic cell lines have been reported. Bioengineered muscle tissues are contractile and respond to electrical and chemical stimulation. In this study we provide an electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues. We focus on Duchenne muscular dystrophy (DMD), a fatal muscle disorder involving the skeletal muscle system. The dystrophin gene, which when mutated causes DMD, encodes for the Dystrophin protein, which anchors the cytoskeletal network inside of a muscle cell to the extracellular matrix outside the cell. Here, we enlist a 3D in vitro model of DMD muscle tissue, to evaluate an understudied aspect of DMD, muscle cell electrical properties uncoupled from presynaptic neural inputs. Our data shows that electrophysiological aspects of DMD are replicated in the 3D bioengineered skeletal muscle tissue model. Furthermore, we test a block co-polymer, poloxamer 188, and demonstrate capacity for improving the membrane potential in DMD muscle. Therefore, this study serves as the baseline for a new in vitro method to examine potential therapies directed at muscular disorders.

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The biphasic and age-dependent impact of Klotho on hallmarks of aging and skeletal muscle function

10.1101/2020.07.22.207043 ◽

2020 ◽

Author(s):

Abish Pius ◽

Zachary Clemens ◽

Sruthi Sivakumar ◽

Amrita Sahu ◽

Sunita Shinde ◽

...

Keyword(s):

Skeletal Muscle ◽

Statistical Physics ◽

Oldest Old ◽

State Parameter ◽

Skeletal Muscle Function ◽

Muscle System ◽

Skeletal Muscle Wasting ◽

Aging Muscle ◽

Network Entropy ◽

Old Mice

ABSTRACTAging is accompanied by a disrupted information flow, which results from accumulation of molecular mistakes. These mistakes ultimately give rise to debilitating disorders such as skeletal muscle wasting, or sarcopenia. To estimate the growing “disorderliness” of the aging muscle system, we employed a statistical physics approach to estimate the state parameter, entropy, as a function of genes associated with hallmarks of aging. Although the most prominent structural and functional alterations were observed in the oldest old mice (27-29 months), we found that the escalating network entropy reached an inflection point at old age (22-24 months). To probe the potential for restoration of molecular “order” and reversal of the sarcopenic phenotype, we overexpressed the longevity protein, α-Klotho. Klotho overexpression modulated genes representing all hallmarks of aging in both old and oldest-old mice. However, whereas Klotho improved strength in old mice, intervention failed to induce a benefit beyond the entropic tipping point.

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Distributed Live Muscle Actuators Controlled by Optical Stimuli

Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments ◽

10.1115/sbc2013-14851 ◽

2013 ◽

Author(s):

Devin Neal ◽

Mahmut Selman Sakar ◽

H. Harry Asada

Keyword(s):

Skeletal Muscle ◽

Genetically Modified ◽

Muscle Cells ◽

Degree Of Freedom ◽

Optical Control ◽

Muscle System ◽

Muscle Actuators

A multi degree of freedom skeletal muscle system stimulated via optical control is presented. These millimeter-scale, optically excitable 3D skeletal muscle bio-actuators are created by culturing genetically modified precursory muscle cells that are activated with light: optogenetics. These muscle bio-actuators are networked together to create a distributed muscle system. Muscle systems can manipulate loads having no fixed joint. These types of loads include shoulders, the mouth, and the jaw.

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Mucopolysaccharidosis type II: skeletal–muscle system involvement

Journal of Pediatric Orthopaedics B ◽

10.1097/bpb.0b013e3283317b7a ◽

2010 ◽

Vol 19 (4) ◽

pp. 313-317 ◽

Cited By ~ 6

Author(s):

Sandra Regina Morini ◽

Carlos Eduardo Steiner ◽

Lelia Britto Passos Gerson

Keyword(s):

Skeletal Muscle ◽

Mucopolysaccharidosis Type ◽

Type Ii ◽

Mucopolysaccharidosis Type Ii ◽

Muscle System ◽

System Involvement

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Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation

Scientific Reports ◽

10.1038/s41598-019-51410-7 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Mi-Ock Baek ◽

Chi Bum Ahn ◽

Hye-Jeong Cho ◽

Ji-Young Choi ◽

Kuk Hui Son ◽

...

Keyword(s):

Skeletal Muscle ◽

Simulated Microgravity ◽

Mrna Level ◽

Mammalian Target Of Rapamycin ◽

Molecular Signature ◽

Autophagic Flux ◽

Dependent Manner ◽

Muscle System ◽

C2c12 Myoblasts ◽

Mechanical Unloading

Abstract The skeletal muscle system has evolved to maintain body posture against a constant gravitational load. Mammalian target of rapamycin (mTOR) regulates the mechanically induced increase in the skeletal muscle mass. In the present study, we investigated mTOR pathway in C2C12 myoblasts in a model of mechanical unloading by creating a simulated microgravity (SM) using 3 D clinorotation. SM decreased the phosphorylation of Akt at Ser 473, which was mediated by mTOR complex 2 (mTORC2), in C2C12 myoblasts, leading to a decrease in the cell growth rate. Subsequently, SM inhibited C2C12 myogenesis in an Akt-dependent manner. In addition, SM increased the phospholipase D (PLD) activity by enhancing PLD2 expression, resulting in the dissociation of mSIN1 from the mTORC2, followed by decrease in the phosphorylation of Akt at Ser 473, and FOXO1 at Ser 256 in C2C12 myoblasts. Exposure to SM decreased the autophagic flux of C2C12 myoblasts by regulation of mRNA level of autophagic genes in a PLD2 and FOXO1-dependent manner, subsequently, resulting in a decrease in the C2C12 myogenesis. In conclusion, by analyzing the molecular signature of C2C12 myogenesis using SM, we suggest that the regulatory axis of the PLD2 induced Akt/FOXO1, is critical for C2C12 myogenesis.

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