Role of NAADP in Coordinating Spatiotemporal Aspects of Calcium Signalling

2003 ◽  
pp. 85-95
Author(s):  
Grant C. Churchill ◽  
Antony Galione
Keyword(s):  
Calcium Signalling

scholarly journals New Insights on the Role of TRP Channels in Calcium Signalling and Immunomodulation: Review of Pathways and Implications for Clinical Practice

2021 ◽  
Author(s):  
Saied Froghi ◽  
Charlotte R. Grant ◽  
Radhika Tandon ◽  
Alberto Quaglia ◽  
Brian Davidson ◽  
...  
Keyword(s):  
Immune System ◽  
Calcium Release ◽  
Transient Receptor Potential ◽  
Trp Channels ◽  
Calcium Influx ◽  
Calcium Signalling ◽  
Chronic Fatigue ◽  
Diverse Range ◽  
Immune System Activation

AbstractCalcium is the most abundant mineral in the human body and is central to many physiological processes, including immune system activation and maintenance. Studies continue to reveal the intricacies of calcium signalling within the immune system. Perhaps the most well-understood mechanism of calcium influx into cells is store-operated calcium entry (SOCE), which occurs via calcium release-activated channels (CRACs). SOCE is central to the activation of immune system cells; however, more recent studies have demonstrated the crucial role of other calcium channels, including transient receptor potential (TRP) channels. In this review, we describe the expression and function of TRP channels within the immune system and outline associations with murine models of disease and human conditions. Therefore, highlighting the importance of TRP channels in disease and reviewing potential. The TRP channel family is significant, and its members have a continually growing number of cellular processes. Within the immune system, TRP channels are involved in a diverse range of functions including T and B cell receptor signalling and activation, antigen presentation by dendritic cells, neutrophil and macrophage bactericidal activity, and mast cell degranulation. Not surprisingly, these channels have been linked to many pathological conditions such as inflammatory bowel disease, chronic fatigue syndrome and myalgic encephalomyelitis, atherosclerosis, hypertension and atopy.

scholarly journals Imaging patterns of calcium transients during neural induction in Xenopus laevis embryos

2000 ◽  
Vol 113 (19) ◽  
pp. 3519-3529 ◽  
Author(s):  
C. Leclerc ◽  
S.E. Webb ◽  
C. Daguzan ◽  
M. Moreau ◽  
A.L. Miller
Keyword(s):  
Xenopus Laevis ◽  
Calcium Signalling ◽  
Neural Induction ◽  
Calcium Transients ◽  
Free Calcium ◽  
Treated Animal ◽  
Cell Stage ◽  
Subsequent Formation ◽  
Xenopus Laevis Embryos

Through the injection of f-aequorin (a calcium-sensitive bioluminescent reporter) into the dorsal micromeres of 8-cell stage Xenopus laevis embryos, and the use of a Photon Imaging Microscope, distinct patterns of calcium signalling were visualised during the gastrulation period. We present results to show that localised domains of elevated calcium were observed exclusively in the anterior dorsal part of the ectoderm, and that these transients increased in number and amplitude between stages 9 to 11, just prior to the onset of neural induction. During this time, however, no increase in cytosolic free calcium was observed in the ventral ectoderm, mesoderm or endoderm. The origin and role of these dorsal calcium-signalling patterns were also investigated. Calcium transients require the presence of functional L-type voltage-sensitive calcium channels. Inhibition of channel activation from stages 8 to 14 with the specific antagonist R(+)BayK 8644 led to a complete inhibition of the calcium transients during gastrulation and resulted in severe defects in the subsequent formation of the anterior nervous system. BayK treatment also led to a reduction in the expression of Zic3 and geminin in whole embryos, and of NCAM in noggin-treated animal caps. The possible role of calcium transients in regulating developmental gene expression is discussed.

scholarly journals Role of Fyn kinase in oocyte developmental potential

2010 ◽  
Vol 22 (6) ◽  
pp. 966 ◽  
Author(s):  
Jinping Luo ◽  
Lynda K. McGinnis ◽  
William H. Kinsey
Keyword(s):  
Calcium Signalling ◽  
Dominant Negative ◽  
Rapid Decline ◽  
Protein Tyrosine Phosphorylation ◽  
Cell Stage ◽  
Developmental Potential ◽  
Developmental Arrest ◽  
Fyn Kinase ◽  
The One

Fyn kinase is highly expressed in oocytes, with inhibitor and dominant-negative studies suggesting a role in the signal transduction events during egg activation. The purpose of the present investigation was to test the hypothesis that Fyn is required for calcium signalling, meiosis resumption and pronuclear congression using the Fyn-knockout mouse as a model. Accelerated breeding studies revealed that Fyn-null females produced smaller litter sizes at longer intervals and exhibited a rapid decline in pup production with increasing age. Fyn-null females produced a similar number of oocytes, but the frequency of immature oocytes and mature oocytes with spindle chromosome abnormalities was significantly higher than in controls. Fertilised Fyn-null oocytes frequently (24%) failed to undergo pronuclear congression and remained at the one-cell stage. Stimulation with gonadotropins increased the number of oocytes ovulated, but did not overcome the above defects. Fyn-null oocytes overexpressed Yes kinase in an apparent effort to compensate for the loss of Fyn, yet still exhibited an altered pattern of protein tyrosine phosphorylation. In summary, Fyn-null female mice exhibit reduced fertility that appears to result from actin cytoskeletal defects rather than calcium signalling. These defects cause developmental arrest during oocyte maturation and pronuclear congression.

Platelet Functions are Decreased in Obesity and Restored after Weight Loss: Evidence for a Role of the SERCA3-Dependent ADP Secretion Pathway

2019 ◽  
Vol 119 (03) ◽  
pp. 384-396 ◽  
Author(s):  
Ziane Elaïb ◽  
Jose Lopez ◽  
Muriel Coupaye ◽  
Kevin Zuber ◽  
Yann Becker ◽  
...  
Keyword(s):  
Weight Loss ◽  
Gastric Bypass Surgery ◽  
Calcium Signalling ◽  
Adenosine Diphosphate ◽  
Control Group ◽  
Obese Patients ◽  
Secretion Pathway ◽  
Healthy Control ◽  
Platelet Functions

AbstractIn obesity, platelets are described as hyperactive, mainly based on increased platelet size and presence of pro-thrombotic plasmatic molecules. We explored platelet functions, including calcium signalling in obesity, and the effect of weight loss. We included 40 obese patients (women with body mass index [BMI] of ≥ 35 kg/m2) who were to undergo gastric bypass surgery and 40 healthy lean subjects (women with BMI of < 25 kg/m2) as a control group. Approximately 1 year after surgery, the obese patients lost weight (75% had a BMI < 35 kg/m2). They were explored a second time with the same healthy control for the same platelet experiments. Compared with controls, obese patients' platelets displayed reduced sensitivity to thrombin (aggregation EC50 increased by 1.9 ± 0.3-fold, p = 0.005) and a lower Ca2+ response (70 ± 7% decrease, p < 10−4). In 17 pairs of patients, we performed additional experiments: in obese patients' platelets, thrombin-induced αIIbβ3 activation was significantly lower (p = 0.003) and sarco-endoplasmic reticulum Ca2+ATPase (SERCA3) expression was decreased (48 ± 6% decrease, p < 10−4). These differences were abolished after weight loss. Interestingly, pharmacological inhibition of SERCA3 activity in control group's platelets mimicked similar alterations than in obese patients' platelets and was associated with defective adenosine diphosphate (ADP) secretion. Addition of ADP to agonist restored platelet functions in obese patients and in SERCA3-inhibited control platelets (five experiments) confirming the direct involvement of the SERCA3-dependent ADP secretion pathway. This is the first study demonstrating that platelets from obese patients are hypo-reactive, due to a deficiency of SERCA3-dependent ADP secretion. Weight loss restores SERCA3 activity and subsequent calcium signalling, αIIbβ3 activation, platelet aggregation and ADP secretion.

scholarly journals Role of the nuclear envelope in calcium signalling

2011 ◽  
Vol 104 (2) ◽  
pp. 70-83 ◽  
Author(s):  
Jean-Pierre Mauger
Keyword(s):  
Nuclear Envelope ◽  
Calcium Signalling

Role of Calcium Signalling in the Development of Pain Syndromes

2005 ◽  
Vol 37 (2) ◽  
pp. 166-171 ◽  
Author(s):  
N. V. Voitenko
Keyword(s):  
Calcium Signalling ◽  
Pain Syndromes

scholarly journals Mitochondrial calcium signalling and cell death: Approaches for assessing the role of mitochondrial Ca2+ uptake in apoptosis

Cell Calcium ◽  
2006 ◽  
Vol 40 (5-6) ◽  
pp. 553-560 ◽  
Author(s):  
György Hajnóczky ◽  
György Csordás ◽  
Sudipto Das ◽  
Cecilia Garcia-Perez ◽  
Masao Saotome ◽  
...  
Keyword(s):  
Cell Death ◽  
Calcium Signalling ◽  
Mitochondrial Calcium

Calcium and cell cycle control in early embryos

Zygote ◽  
1996 ◽  
Vol 4 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Martin Wilding
Keyword(s):  
Cell Cycle ◽  
Major Part ◽  
Cell Cycle Control ◽  
Calcium Signalling ◽  
Entry And Exit ◽  
The Past ◽  
New Concepts ◽  
Early Embryos ◽  
M Phase

Over the past few years, we have witnessed a burgeoning series of papers addressing the role of calcium signalling in cell cycle control. In this review I will attempt to bring together all the diverse threads and discuss new concepts that have arisen from the most recent data. Because the major part of the data concerns mitosis/meiosis entry and exit, I have focused on these areas. I will jointly refer to meiotic and mitotic phases of the cell cycle as M-phase because these phases are highly comparable. Studies of the cell cycle involve a huge range of species, from plants to humans. I will, however, restrict this review to the work performed in early embryos. I apologise in advance to contributors to this field whose names I do not mention because they do not work on embryos.

scholarly journals 5-Hydroxytryptamine-induced calcium-channel gating in rainbow trout (Oncorhynchus mykiss) peripheral blood lymphocytes

1997 ◽  
Vol 323 (1) ◽  
pp. 251-258 ◽  
Author(s):  
François FERRIERE ◽  
Naïm A. KHAN ◽  
Jean P. MEYNIEL ◽  
Pierre DESCHAUX
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Peripheral Blood ◽  
Calcium Signalling ◽  
Peripheral Blood Lymphocytes ◽  
Free Calcium ◽  
Receptor Subtype ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Peripheral Blood Lympho

The present study was conducted on peripheral blood lympho-cytes of rainbow trout (Oncorhynchus mykiss) to assess the role of 5-hydroxytryptamine (5-HT; ‘serotonin’) in calcium signalling. 5-HT-induced increases in intracellular free calcium concentrations, [Ca2+]i, and its action was mediated by 5-HT receptor subtype 3 (5-HT3), but not by 5-HT receptor subtype 1A (5-HT1A) or subtype 2 (5-HT2) in these cells. In Ca2+-containing medium (1 mM CaCl2), 5-HT and 2-methyl-5-HT (5-HT3 receptor agonist) induced increases in [Ca2+]i, whereas in Ca2+-free medium (0 Ca2+, 1 mM EGTA), these two agents failed to evoke increases in [Ca2+]i in these cells, demonstrating that 5-HT mobilizes Ca2+ from the extracellular environment. Furthermore, 5-HT-induced increases in [Ca2+]i are not contributed to by the intracellular endoplasmic reticulum (ER) pool, as thapsigargin, an agent that recruits Ca2+ from ER stores, had additive effects on 5-HT-induced [Ca2+]i responses in fish peripheral lymphocytes. 5-HT-induced increases in [Ca2+]i were mediated by 5-HT3 receptors via gating the calcium through L-type, but not N-type, calcium channels in trout lymphocytes.

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