Vpr and Its Interactions with Cellular Proteins

2009 ◽  
pp. 177-200 ◽  
Author(s):  
Vicente Planelles ◽  
Serge Benichou
Keyword(s):  
Cellular Proteins

PHOTACs Enable Optical Control of Protein Degradation

2019 ◽  
Author(s):  
Martin Reynders ◽  
Bryan Matsuura ◽  
Marleen Bérouti ◽  
Daniele Simoneschi ◽  
Antonio Marzio ◽  
...  
Keyword(s):  
Protein Degradation ◽  
E3 Ligase ◽  
Optical Control ◽  
Cellular Proteins ◽  
Bifunctional Molecules ◽  
Protein Levels ◽  
Lead Compounds ◽  
Subsequent Degradation ◽  
Temporal And Spatial ◽  
Precision Therapeutics

<p><i>PROTACs (proteolysis targeting chimeras) are bifunctional molecules that tag proteins for ubiquitylation by an E3 ligase complex and subsequent degradation by the proteasome. They have emerged as powerful tools to control the levels of specific cellular proteins and are on the verge of being clinically used. We now introduce photoswitchable PROTACs that can be activated with the temporal and spatial precision that light provides. These trifunctional molecules, which we named PHOTACs, consist of a ligand for an E3 ligase, a photoswitch, and a ligand for a protein of interest. We demonstrate this concept by using PHOTACs that target either BET family proteins (BRD2,3,4) or FKBP12. Our lead compounds display little or no activity in the dark but can be reversibly activated to varying degrees with different wavelengths of light. Our modular and generalizable approach provides a method for the optical control of protein levels with photopharmacology and could lead to new types of precision therapeutics that avoid undesired systemic toxicity.</i><b></b></p>

scholarly journals Why do cellular proteins linked to K63-polyubiquitin chains not associate with proteasomes?

2013 ◽  
Vol 32 (4) ◽  
pp. 552-565 ◽  
Author(s):  
James A Nathan ◽  
Hyoung Tae Kim ◽  
Lily Ting ◽  
Steven P Gygi ◽  
Alfred L Goldberg
Keyword(s):  
Cellular Proteins ◽  
Polyubiquitin Chains

Proteomic analysis of host cellular proteins co-immunoprecipitated with duck enteritis virus gC

2021 ◽  
Vol 245 ◽  
pp. 104281
Author(s):  
Liu Chen ◽  
Zheng Ni ◽  
Jionggang Hua ◽  
Weicheng Ye ◽  
Keshu Liu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Duck Enteritis Virus ◽  
Cellular Proteins ◽  
Enteritis Virus

scholarly journals Gly-Gly-X, a Novel Consensus Sequence for the Proteolytic Processing of Viral and Cellular Proteins

1989 ◽  
Vol 264 (16) ◽  
pp. 9107-9110
Author(s):  
C López-Otín ◽  
C Simón-Mateo ◽  
L Martínez ◽  
E Viñuela
Keyword(s):  
Consensus Sequence ◽  
Proteolytic Processing ◽  
Cellular Proteins

scholarly journals Intracellular Routing and Recognition of Lipid-Based mRNA Nanoparticles

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 945
Author(s):  
Christophe Delehedde ◽  
Luc Even ◽  
Patrick Midoux ◽  
Chantal Pichon ◽  
Federico Perche
Keyword(s):  
Gene Therapy ◽  
Immune Responses ◽  
Transient Expression ◽  
Messenger Rna ◽  
Lipid Nanoparticles ◽  
Delivery Systems ◽  
Clinical Applications ◽  
Cellular Proteins ◽  
Mrna Sequence ◽  
Cytoplasmic Expression

Messenger RNA (mRNA) is being extensively used in gene therapy and vaccination due to its safety over DNA, in the following ways: its lack of integration risk, cytoplasmic expression, and transient expression compatible with fine regulations. However, clinical applications of mRNA are limited by its fast degradation by nucleases, and the activation of detrimental immune responses. Advances in mRNA applications, with the recent approval of COVID-19 vaccines, were fueled by optimization of the mRNA sequence and the development of mRNA delivery systems. Although delivery systems and mRNA sequence optimization have been abundantly reviewed, understanding of the intracellular processing of mRNA is mandatory to improve its applications. We will focus on lipid nanoparticles (LNPs) as they are the most advanced nanocarriers for the delivery of mRNA. Here, we will review how mRNA therapeutic potency can be affected by its interactions with cellular proteins and intracellular distribution.

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