EBV Replication Enzymes

2001 ◽  
pp. 65-87 ◽  
Author(s):  
T. Tsurumi
Keyword(s):  
Replication Enzymes

New Strategy on Antimicrobial-resistance: Inhibitors of DNA Replication Enzymes

2019 ◽  
Vol 26 (10) ◽  
pp. 1761-1787 ◽  
Author(s):  
Lanhua Yi ◽  
Xin Lü
Keyword(s):  
Dna Replication ◽  
Antimicrobial Resistance ◽  
Enzyme Inhibitors ◽  
Dna Gyrase ◽  
Dna Helicase ◽  
Type Ii ◽  
New Strategy ◽  
Clinic Practice ◽  
Living Organisms ◽  
Replication Enzymes

Background:Antimicrobial resistance is found in all microorganisms and has become one of the biggest threats to global health. New antimicrobials with different action mechanisms are effective weapons to fight against antibiotic-resistance.Objective:This review aims to find potential drugs which can be further developed into clinic practice and provide clues for developing more effective antimicrobials.Methods:DNA replication universally exists in all living organisms and is a complicated process in which multiple enzymes are involved in. Enzymes in bacterial DNA replication of initiation and elongation phases bring abundant targets for antimicrobial development as they are conserved and indispensable. In this review, enzyme inhibitors of DNA helicase, DNA primase, topoisomerases, DNA polymerase and DNA ligase were discussed. Special attentions were paid to structures, activities and action modes of these enzyme inhibitors.Results:Among these enzymes, type II topoisomerase is the most validated target with abundant inhibitors. For type II topoisomerase inhibitors (excluding quinolones), NBTIs and benzimidazole urea derivatives are the most promising inhibitors because of their good antimicrobial activity and physicochemical properties. Simultaneously, DNA gyrase targeted drugs are particularly attractive in the treatment of tuberculosis as DNA gyrase is the sole type II topoisomerase in Mycobacterium tuberculosis. Relatively, exploitation of antimicrobial inhibitors of the other DNA replication enzymes are primeval, in which inhibitors of topo III are even blank so far.Conclusion:This review demonstrates that inhibitors of DNA replication enzymes are abundant, diverse and promising, many of which can be developed into antimicrobials to deal with antibioticresistance.

Expression patterns of DNA replication enzymes and the regulatory factor DREF during Drosophila development analyzed with specific antibodies

1995 ◽  
Vol 85 (2-3) ◽  
pp. 147-155 ◽  
Author(s):  
Masamitsu Yamaguchi ◽  
Fumiko Hirose ◽  
Yoshio Nishimoto ◽  
Toyobumi Naruge ◽  
Masako Ikeda ◽  
...  
Keyword(s):  
Dna Replication ◽  
Expression Patterns ◽  
Regulatory Factor ◽  
Drosophila Development ◽  
Specific Antibodies ◽  
Replication Enzymes

scholarly journals Glycolytic pyruvate kinase moonlighting activities in DNA replication initiation and elongation

2020 ◽  
Author(s):  
Steff Horemans ◽  
Matthaios Pitoulias ◽  
Alexandria Holland ◽  
Panos Soultanas ◽  
Laurent Janniere
Keyword(s):  
Dna Replication ◽  
Metabolic Control ◽  
Pyruvate Kinase ◽  
Metabolic Activity ◽  
Growth Conditions ◽  
Replication Initiation ◽  
Terminal Domain ◽  
Wide Range ◽  
Replication Enzymes

SUMMARYCells have evolved a metabolic control of DNA replication to respond to a wide range of nutritional conditions. Accumulating data suggest that this poorly understood control depends, at least in part, on Central Carbon Metabolism (CCM). In Bacillus subtilis, the glycolytic pyruvate kinase (PykA) is intricately linked to replication. This 585 amino-acid-long enzyme comprises a catalytic (Cat) domain that binds to phosphoenolpyruvate (PEP) and ADP to produce pyruvate and ATP, and a C-terminal domain of unknown function. Interestingly, the C-terminal domain termed PEPut interacts with Cat and is homologous a domain that, in other metabolic enzymes, are phosphorylated at a conserved TSH motif at the expense of PEP and ATP to drive sugar import and catalytic or regulatory activities. To gain insights into the role of PykA in replication, DNA synthesis was analyzed in various Cat and PEPut mutants grown in a medium where the metabolic activity of PykA is dispensable for growth. Measurements of replication parameters (ori/ter ratio, C period and fork speed) and of the pyruvate kinase activity showed that PykA mutants exhibit replication defects resulting from side chain modifications in the PykA protein rather than from a reduction of its metabolic activity. Interestingly, Cat and PEPut have distinct commitments in replication: while Cat impacts positively and negatively replication fork speed, PEPut stimulates initiation through a process depending on Cat-PEPut interaction and growth conditions. Residues binding to PEP and ADP in Cat, stabilizing the Cat-PEPut interaction and belonging to the TSH motif of PEPut were found important for the commitment of PykA in replication. In vitro, PykA affects the activities of replication enzymes (the polymerase DnaE, helicase DnaC and primase DnaG) essential for initiation and elongation and genetically linked to pykA. Our results thus connect replication initiation and elongation to CCM metabolites (PEP, ATP and ADP), critical Cat and PEPut residues and to multiple links between PykA and the replication enzymes DnaE, DnaC and DnaG. We propose that PykA is endowed with a moonlighting activity that senses the concentration of signaling metabolites and interacts with replication enzymes to convey information on the cellular metabolic state to the replication machinery and adjust replication initiation and elongation to metabolism. This defines a new type of replication regulator proposed to be part of the metabolic control that gates replication in the cell cycle.

Eukaryotic DNA replication. Enzymes and proteins acting at the fork

1990 ◽  
Vol 194 (3) ◽  
pp. 699-712 ◽  
Author(s):  
Pia THOMMES ◽  
Ulrich HUBSCHER
Keyword(s):  
Dna Replication ◽  
Eukaryotic Dna ◽  
Replication Enzymes

scholarly journals Break-join recombination in phage lambda.

Genetics ◽  
1990 ◽  
Vol 125 (3) ◽  
pp. 463-474 ◽  
Author(s):  
F W Stahl ◽  
M S Fox ◽  
D Faulds ◽  
M M Stahl
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Restriction Enzyme ◽  
Restriction Site ◽  
Phage Lambda ◽  
Double Chain ◽  
Exchange Event ◽  
Break Site ◽  
Replication Enzymes

Abstract In phage lambda, when DNA replication is blocked, recombination mediated by the Red pathway occurs only near the double-chain break site, cos, that defines the termini of the virion chromosome. The recombinants initiated by cos contain newly synthesized DNA near cos, in amount corresponding to a few percent of the length of lambda. A restriction enzyme cut delivered to one parent far from cos results in elevated recombination near the restriction site. Recombinants induced by this cut have a similarly small amount of DNA synthesis in these replication-blocked crosses. When restriction cuts are introduced in the presence of normal amounts of all of the DNA replication enzymes, many of the resulting recombinants still enjoy, at most, a small amount of DNA synthesis associated with the exchange event. Thus, these experiments fail to support the previously considered possibility that Red-mediated recombination in lambda proceeds largely through a break-copy pathway.

scholarly journals Potential Antivirals: Natural Products Targeting Replication Enzymes of Dengue and Chikungunya Viruses

2017 ◽  
Author(s):  
Ana Flávia Costa da Silveira Oliveira ◽  
Róbson Ricardo Teixeira ◽  
André Silva de Oliveira ◽  
Ana Paula Martins de Souza ◽  
Milene Lopes da Silva ◽  
...  
Keyword(s):  
Natural Products ◽  
Dengue Virus ◽  
Chikungunya Virus ◽  
The Past ◽  
Antiviral Compounds ◽  
Replication Enzymes

Dengue virus (DENV) and chikungunya virus (CHIKV) are reemergent arboviruses that are transmitted by mosquitoes of the Aedes genus. During the last several decades, these viruses have been responsible for millions of cases of infection and thousands of deaths worldwide. Therefore, several investigations were conducted over the past few years to find antiviral compounds for the treatment of DENV and CHIKV infections. One attractive strategy is the screening of compounds that target enzymes involved in the replication of both DENV and CHIKV. In this review, we describe advances in the evaluation of natural products targeting the enzymes involved in the replication of these viruses.

scholarly journals Teicoplanin is a potential inhibitor of SARS CoV-2 replication enzymes: A docking study

2020 ◽  
Vol 10 (12) ◽  
pp. 563
Author(s):  
MuhammadAsim Raza Basra ◽  
Aatika Sadia ◽  
Muhammad Azam
Keyword(s):  
Docking Study ◽  
Potential Inhibitor ◽  
Replication Enzymes
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