Altered Cell Surface Glycosphingolipids of Murine Lymphocytes Belonging to Different Differentiation Pathways

1982 ◽  
pp. 199-207 ◽  
Author(s):  
P. F. Mühlradt ◽  
B. Kniep ◽  
R. G. Gruner
Keyword(s):  
Cell Surface ◽  
Altered Cell ◽  
Differentiation Pathways

Surface morphology and agglutination of lectin-treated human lymphocytes and lymphoblasts

1976 ◽  
Vol 21 (3) ◽  
pp. 563-578
Author(s):  
J.H. Temmink ◽  
J.G. Collard ◽  
J. Roosien ◽  
J.F. Van den Bosch
Keyword(s):  
Cell Surface ◽  
Surface Morphology ◽  
Human Lymphocytes ◽  
Cell Type ◽  
A Cell ◽  
Human Peripheral Lymphocytes ◽  
Normal Human ◽  
Altered Cell ◽  
Induced Changes ◽  
Cell Surface Morphology

Two human lymphoblasts (Raji and EB3) and normal human peripheral lymphocytes were exposed to different concentrations of Concanavalin A and wheat germ agglutinin. The lectin-induced agglutination was determined and correlated with lectin-induced changes in the surface morphology of these cells as studied in a scanning electron microscope. Whenever the lectin induced high agglutinability in a cell type, it also invariably had a smoothing effect on the cell surface. In contrast, when cells did not agglutinate well with a certain lectin, their cell surface remained essentially rough (villous) after addition of the lectin. The correlation found between increased agglutinability and altered cell surface morphology upon treatment with certain lectins suggests that both phenomena result from one and the same process. Additional evidence for this postulate is presented.

scholarly journals Altered Cell Surface N-Glycosylation of Resting and Activated T Cells in Systemic Lupus Erythematosus

2019 ◽  
Vol 20 (18) ◽  
pp. 4455 ◽  
Author(s):  
Enikő Szabó ◽  
Ákos Hornung ◽  
Éva Monostori ◽  
Márta Bocskai ◽  
Ágnes Czibula ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
T Cells ◽  
T Cell ◽  
Cell Surface ◽  
Lupus Erythematosus ◽  
Lectin Binding ◽  
Systemic Lupus ◽  
Activated T Cells ◽  
Neuraminidase Treatment ◽  
Altered Cell

Altered cell surface glycosylation in congenital and acquired diseases has been shown to affect cell differentiation and cellular responses to external signals. Hence, it may have an important role in immune regulation; however, T cell surface glycosylation has not been studied in systemic lupus erythematosus (SLE), a prototype of autoimmune diseases. Analysis of the glycosylation of T cells from patients suffering from SLE was performed by lectin-binding assay, flow cytometry, and quantitative real-time PCR. The results showed that resting SLE T cells presented an activated-like phenotype in terms of their glycosylation pattern. Additionally, activated SLE T cells bound significantly less galectin-1 (Gal-1), an important immunoregulatory lectin, while other lectins bound similarly to the controls. Differential lectin binding, specifically Gal-1, to SLE T cells was explained by the increased gene expression ratio of sialyltransferases and neuraminidase 1 (NEU1), particularly by elevated ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1)/NEU1 and ST3 beta-galactoside alpha-2,3-sialyltransferase 6 (ST3GAL6)/NEU1 ratios. These findings indicated an increased terminal sialylation. Indeed, neuraminidase treatment of cells resulted in the increase of Gal-1 binding. Altered T cell surface glycosylation may predispose the cells to resistance to the immunoregulatory effects of Gal-1, and may thus contribute to the pathomechanism of SLE.

scholarly journals Differential Association of HLA-B*2705 and B*2709 to Ankylosing Spondylitis Correlates with Limited Peptide Subsets but Not with Altered Cell Surface Stability

2002 ◽  
Vol 277 (32) ◽  
pp. 28749-28756 ◽  
Author(s):  
Manuel Ramos ◽  
Alberto Paradela ◽  
Miriam Vázquez ◽  
Anabel Marina ◽  
Jesús Vázquez ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Cell Surface ◽  
Differential Association ◽  
Surface Stability ◽  
Altered Cell

scholarly journals Production of Exopolysaccharide byBurkholderia cenocepaciaResults in Altered Cell‐Surface Interactions and Altered Bacterial Clearance in Mice

2004 ◽  
Vol 190 (5) ◽  
pp. 957-966 ◽  
Author(s):  
Barbara‐Ann D. Conway ◽  
Karen K. Chu ◽  
Johan Bylund ◽  
Eleonora Altman ◽  
David P. Speert
Keyword(s):  
Cell Surface ◽  
Surface Interactions ◽  
Bacterial Clearance ◽  
Cell Surface Interactions ◽  
Altered Cell

Altered cell surface carbohydrates and metastatic potential in variants of B16 mouse melanoma

1987 ◽  
Vol 23 (11) ◽  
pp. 1748-1749
Author(s):  
J. Finne ◽  
H. Haidvogl ◽  
S. Castori ◽  
T. Feizi ◽  
M.M. Burger
Keyword(s):  
Cell Surface ◽  
Metastatic Potential ◽  
Mouse Melanoma ◽  
Altered Cell ◽  
B16 Mouse Melanoma ◽  
Surface Carbohydrates

scholarly journals Altered cell-surface protein(s), crossreactive with DNA, on spleen cells of autoimmune lupic mice.

1987 ◽  
Vol 84 (5) ◽  
pp. 1361-1363 ◽  
Author(s):  
L. Jacob ◽  
M. A. Lety ◽  
R. C. Monteiro ◽  
F. Jacob ◽  
J. F. Bach ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Protein ◽  
Protein S ◽  
Cell Surface Protein ◽  
Spleen Cells ◽  
Altered Cell
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