The Avian Embryo as a Model System for Skeletal Myogenesis

2014 ◽  
pp. 99-122 ◽  
Author(s):  
Claire E. Hirst ◽  
Christophe Marcelle
Keyword(s):  
Model System ◽  
Avian Embryo ◽  
Skeletal Myogenesis

Changes in the trace element concentrations of developing chick embryo brain tissue

1993 ◽  
Vol 1993 ◽  
pp. 163-163
Author(s):  
A. MacPherson ◽  
J. Dixon ◽  
R.C. Noble ◽  
B. Speake ◽  
M. Cocchi
Keyword(s):  
Model System ◽  
Structure And Function ◽  
Superoxide Dismutases ◽  
Avian Embryo ◽  
Membrane Structure And Function ◽  
Free Radical Attack ◽  
And Function ◽  
Radical Attack ◽  
Embryo Brain ◽  
Pufa Metabolism

Polyunsaturated fatty acids(PUFA) are vitally important for normal neonatal development. As well as providing a source of energy they are intrinsically involved in the establishment and maintenance of cell membrane structure and function. High PUFA levels, however, are subject to peroxidation and consequently require to be protected against free radical attack via an adequate antioxidant supply. The avian embryo was used as a model system as it is particularly dependent on PUFA and poor hatchability has recently been linked to inadequate PUFA metabolism. The micronutrient elements Se, Cu, Zn, Fe and Mn are involved in the antioxidant complex through their presence in the enzymes glutathione peroxidase (GSH-PX), superoxide dismutases (SOD) and catalase. The concentrations of these elements were measured in eggs and in chick embryonic and post hatch brains at different stages of development in order to elucidate their roles in the maintenance of PUFA metabolism.

Cell adhesion mechanisms in gangliogenesis studied in avian embryo and in a model system

1985 ◽  
Vol 17 (4) ◽  
pp. 247-260 ◽  
Author(s):  
Hirohiko Aoyama ◽  
Annie Delouvée ◽  
Jean Paul Thiery
Keyword(s):  
Cell Adhesion ◽  
Model System ◽  
Avian Embryo

The avian embryo: a powerful model system for studying neural induction

1994 ◽  
Vol 8 (10) ◽  
pp. 687-691 ◽  
Author(s):  
Claudio D. Stern
Keyword(s):  
Neural Induction ◽  
Model System ◽  
Avian Embryo

A Protozoan Model for Golgi-Associated Calcification

1971 ◽  
Vol 29 ◽  
pp. 332-333
Author(s):  
D. C. Williams ◽  
D. E. Outka
Keyword(s):  
Golgi Apparatus ◽  
Organic Matrix ◽  
Model System ◽  
Sequential Formation

Many studies have shown that the Golgi apparatus is involved in a variety of synthetic activities, and probably no Golgi product is more elaborate than the scales produced by various kinds of phytoflagellates. The formation of calcified scales (coccoliths, Fig. 1,2) of the coccolithophorid phytoflagellates provides a particularly interesting model system for the study of biological mineralization, and the sequential formation of Golgi products.The coccoliths of Hymenomonas carterae consist of a scale-like base (Fig. 2 and 4, b) with a highly structured calcified (CaCO3) rim composed of two distinct elements which alternate about the base periphery (Fig. 1 and 3, A, B). Each element is enveloped by a sheath-like organic matrix (Fig. 3; Fig. 4, m).

Existence of DNA in yeast microbodies

1978 ◽  
Vol 36 (2) ◽  
pp. 232-233
Author(s):  
Masako Osumi ◽  
Misuzu Nagano ◽  
Hiroko Kazama
Keyword(s):  
Catalase Activity ◽  
Buoyant Density ◽  
Model System ◽  
Contour Length ◽  
Native State ◽  
Normal Alkane ◽  
Remarkable Increase ◽  
Dna Molecule ◽  
Mitochondrial Dnas

We have found that microbodies appeared profusely together with a remarkable increase in catalase activity in normal alkane-grown cells of hydrocarbon-utilizing Candida yeasts, and that the microbodies multiplied by division in these cells. These features of Candida yeasts seem to provide a useful model system for studies on the biogenesis of the microbody. Subsequently, we have succeeded in isolation of Candida microbodies in an apparently native state, as judged biochemically and morphologically. The presence of DNA in the purified microbody fraction thus obtained was proved by the diphenylamine method. DNA molecule of about 15 urn in contour length was released from an isolated microbody. The physicochemical analyses of the microbody DNA revealed that its buoyant density differed from nuclear and mitochondrial DNAs. All these results lead us to the possibility that there is a novel type of DNA in microbodies.

The coprecipitation of Cr3P and Cr in Cu

1988 ◽  
Vol 46 ◽  
pp. 764-765
Author(s):  
M.J. Witcomb ◽  
U. Dahmen ◽  
K.H. Westmacott
Keyword(s):  
Orientation Relationship ◽  
Interface Structure ◽  
Model System ◽  
Relative Orientation ◽  
Age Hardening ◽  
Precipitation Behavior ◽  
Solution Treated ◽  
Small Needle ◽  
Diffraction Patterns ◽  
Interface Structures

Cu-Cr age-hardening alloys are of interest as a model system for the investigation of fcc/bcc interface structures. Several past studies have investigated the morphology and interface structure of Cr precipitates in a Cu matrix (1-3) and good success has been achieved in understanding the crystallography and strain contrast of small needle-shaped precipitates. The present study investigates the effect of small amounts of phosphorous on the precipitation behavior of Cu-Cr alloys.The same Cu-0.3% Cr alloy as was used in earlier work was rolled to a thickness of 150 μm, solution treated in vacuum at 1050°C for 1h followed by quenching and annealing for various times at 820 and 863°C.Two laths and their corresponding diffraction patterns in an alloy aged 2h at 820°C are shown in correct relative orientation in Fig. 1. To within the limit of accuracy of the diffraction patterns the orientation relationship was that of Kurdjumov-Sachs (KS), i.e. parallel close-packed planes and directions.

Membrane-associated microtubular areays induced in proximal myelinated processes of dorsal root ganglia by large doses of vitamin B6

1986 ◽  
Vol 44 ◽  
pp. 368-369
Author(s):  
V.J. Montpetit ◽  
S. Dancea ◽  
L. Tryphonas ◽  
D.F. Clapin
Keyword(s):  
Animal Model ◽  
Endoplasmic Reticulum ◽  
Dorsal Root Ganglia ◽  
Rough Endoplasmic Reticulum ◽  
Dorsal Root ◽  
Vitamin B6 ◽  
Morphological Changes ◽  
Model System ◽  
Sensory Nerves ◽  
Peripheral Sensory

Very large doses of pyridoxine (vitamin B6) are neurotoxic in humans, selectively affecting the peripheral sensory nerves. We have undertaken a study of the morphological and biochemical aspects of pyridoxine neurotoxicity in an animal model system. Early morphological changes in dorsal root ganglia (DRG) associated with pyridoxine megadoses include proliferation of neurofilaments, ribosomes, rough endoplasmic reticulum, and Golgi complexes. We present in this report evidence of the formation of unique aggregates of microtubules and membranes in the proximal processes of DRG which are induced by high levels of pyridoxine.

An Ultrastructural Comparison of Hepatocytes from ADH+ and ADH−Peromyscus Maniculatus

1996 ◽  
Vol 54 ◽  
pp. 30-31
Author(s):  
J. T. Ellzey ◽  
D. Borunda ◽  
B. P. Stewart
Keyword(s):  
South Carolina ◽  
Adult Male ◽  
Uranyl Acetate ◽  
Model System ◽  
Deer Mice ◽  
En Bloc ◽  
Genetic Stock ◽  
Hepes Buffer ◽  
Transmission Electron ◽  
Color Scanner

Genetically alcohol deficient deer mice (ADHN/ADHN) (obtained from the Peromyscus Genetic Stock Center, Univ. of South Carolina) lack hepatic cytosolic alcohol dehydrogenase. In order to determine if these deer mice would provide a model system for an ultrastructural study of the effects of ethanol on hepatocyte organelles, 75 micrographs of ADH+ adult male deer mice (n=5) were compared with 75 micrographs of ADH− adult male deer mice (n=5). A morphometric analysis of mitochondrial and peroxisomal parameters was undertaken.The livers were perfused with 0.1M HEPES buffer followed by 0.25% glutaraldehyde and 2% sucrose in 0.1M HEPES buffer (4C), removed, weighed and fixed by immersion in 2.5% glutaraldehyde in 0.1M HEPES buffer, pH 7.4, followed by a 3,3’ diaminobenzidine (DAB) incubation, postfixation with 2% OsO4, en bloc staining with 1% uranyl acetate in 0.025M maleate-NaOH buffer, dehydrated, embedded in Poly/Bed 812-BDMA epon resin, sectioned and poststained with uranyl acetate and lead citrate. Photographs were taken on a Zeiss EM-10 transmission electron microscope, scanned with a Howtek personal color scanner, analyzed with OPTIMAS 4.02 software on a Gateway2000 4DX2-66V personal computer and stored in Excel 4.0.

Elemental analysis of human erythrocytes

1989 ◽  
Vol 47 ◽  
pp. 242-243
Author(s):  
S. A. Livesey ◽  
A. A. del Campo ◽  
E. S. Griffey ◽  
D. Ohlmer ◽  
T. Schifani ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Elemental Analysis ◽  
Human Erythrocyte ◽  
Spectroscopic Analysis ◽  
Model System ◽  
Human Erythrocytes ◽  
List Type ◽  
Chemical Fixation ◽  
Ethanol Dehydration ◽  
Lowicryl K4m

The aim of this study is to compare methods of sample preparation for elemental analysis. The model system which is used is the human erythrocyte. Energy dispersive spectroscopic analysis has been previously reported for cryofixed and cryosectioned erythrocytes. Such work represents the reference point for this study. The use of plastic embedded samples for elemental analysis has also been documented. The work which is presented here is based on human erythrocytes which have been either chemically fixed and embedded or cryofixed and subsequently processed by a variety of techniques which culminated in plastic embedded samples.Heparinized and washed erythrocytes were prepared by the following methods for this study :(1). Chemical fixation in 4% paraformaldehyde/0.25% glutaraldehyde/0.2 M sucrose in 0.1 M Na cacodylate, pH 7.3 for 30 min, followed by ethanol dehydration, infiltration and embedding in Lowicryl K4M at -20° C.

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