Superoxide Radical Scavenging Activities of Wines, and Antioxidative Properties of Fractions Recovered from Merlot Wine Pomace

1997 ◽  
pp. 359-364
Author(s):  
Michikatsu Sato ◽  
Narasimhan Ramarathnam ◽  
Yumiko Suzuki ◽  
Tetsuro Ohkubo ◽  
Masao Takeuchi ◽  
...  
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging ◽  
Antioxidative Properties

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

scholarly journals Ultrasound-assisted extraction of protein from Bombay locusts and its impact on functional and antioxidative properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Passakorn Kingwascharapong ◽  
Manat Chaijan ◽  
Supatra Karnjanapratum
Keyword(s):  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Surface Hydrophobicity ◽  
Molecular Characteristics ◽  
Ultrasound Assisted Extraction ◽  
Antioxidative Properties ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Nonessential Amino Acids ◽  
The Impact

AbstractImpact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40–60%) and times (10–30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50–60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.

Hydroxyl and superoxide radical scavenging abilities of chromonyl-thiazolidine-2,4-dione compounds

Luminescence ◽  
2009 ◽  
Vol 24 (2) ◽  
pp. 96-101 ◽  
Author(s):  
Irena Kruk ◽  
Oya Bozdağ-Dündar ◽  
Rahmiye Ertan ◽  
Hassan Y. Aboul-Enein ◽  
Teresa Michalska
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging

scholarly journals Radical scavenging activity of crude polysaccharides from Camellia sinensis

2011 ◽  
Vol 63 (3) ◽  
pp. 717-721
Author(s):  
Fan Yang ◽  
Zhiwei Yang ◽  
Jianbo Xiao
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Camellia Sinensis ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Molecular Weights

A preparation of crude polysaccharides (TPS) was isolated from Camellia sinensis by precipitation and ultrafiltration. TPS1, TPS2, and TPS3 had molecular weights of 240, 21.4, and 2.46 kDa, respectively. The radical scavenging activities of TPS were evaluated by DPPH free radical, hydroxyl radical and superoxide radical scavenging. These results revealed that TPS exhibited strong radical scavenging activity in a concentration-dependent manner. TPS3 with lowest molecular weight showed a higher radical scavenging activity.

scholarly journals Analysis of Total Antioxidant Capacities of Various Seaweeds Grown off the Korean Coast (P06-110-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Chai-Hyeon Lee ◽  
Ye-Na Park ◽  
Hyeung-Rak Kim ◽  
Sang Gil Lee ◽  
Bohkyung Kim
Keyword(s):  
Functional Food ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Antioxidant Capacities ◽  
Scavenging Capacity ◽  
Total Antioxidant ◽  
Radical Generation ◽  
Dpph Radical Scavenging

Abstract Objectives It has been a long time since seaweeds have been used for food ingredients in Asian countries. Recently, a body of research has revealed the health benefits of bioactive compounds in seaweeds, especially their antioxidant capacities. Although numerous seaweeds inhabit in the ocean, only a small percentage has been explored for functional food. Therefore, the purpose of this study was to analyze the antioxidant capacities of various seaweeds grown off the Korean coast to screen for the potential functional food sources. Methods Ten ethanol extracts of Korean seaweeds were provided by the National Marine Biodiversity Institute of Korea, which included Scytosiphon gracilis, Scytosiphon lomentaria, Sargassum muticum, Sargassum confusum, Petrospongium rugosum, Sargassum fusiforme, Petalonia fascia, Sargassum nigrifolium, Ishige foliacea, and Myelophycus simplex. Hundred mg/L of samples in 80% methanol was used to measure their ABTS, DPPH, and superoxide radical scavenging activities. Mg vitamin C equivalent antioxidant capacity (VCEAC)/100 mg was used to express the ABTS and DPPH radical scavenging capacities. For the superoxide radical scavenging capacity, inhibition rate of superoxide radical generation (%) was calculated. Results Among the ten seaweeds, S. nigrifolium and I. foliacea exhibited the most significant radical scavenging capacities. DPPH radical scavenging capacities of I. foliacea and S. nigrifolium were 122.4 mg VCEAC/100 mg and 95.8 mg VCEAC/100 mg, respectively. For ABTS radical scavenging capacity, I. foliacea exhibited 178.5 mg VCEAC/100 mg, followed by S. nigrifolium as 80.9 mg VCEAC/100 mg. I. foliacea inhibited about 68% of superoxide radical generation followed by S. nigrifolium (40.6%) while vitamin C as positive control inhibited about 28.9% of superoxide radical generation. The antioxidant capacities measured by the three assays were positively correlated with each other. Conclusions The current study explored total antioxidant capacities of various Korean seaweeds and found I. foliacea and S. nigrifolium as the most potential antioxidant-rich food resources. Further research would be warranted to investigate bioactive compounds from S. nigrifolium and I. foliacea. Funding Sources This work was supported by the Pukyong National University Research Fund in 2018.

scholarly journals IN VITRO ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF METHANOLIC EXTRACTS OF UVARIA CHAMAE LEAVES AND ROOTS

2019 ◽  
Vol 11 (1) ◽  
pp. 67
Author(s):  
Nwokolo Lorreta Nwakaego ◽  
Onyekwelu Kenechukwu Chibuike ◽  
Ene Martin Chukwugekwu ◽  
Adilieje Chioma Marylyn ◽  
Ezechukwu Ifunanya Ngozi ◽  
...  
Keyword(s):  
Free Radical ◽  
Vitamin E ◽  
Vitamin A ◽  
Vitamin C ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Root Extract ◽  
Leaves And Roots

Objective: This study investigated the in vitro antioxidant and free radical potentials of methanol extracts of Uvaria chamae leaves and roots.Methods: Fresh Uvaria chamae leaves and roots were air dried, pulverized and extracted using methanol. Phytochemical, total phenolic, flavonoids, antioxidant and tannin contents, DPPH, hydroxyl, and superoxide radical scavenging properties of the extracts were determined using standard methods.Results: In vitro antioxidant potentials revealed that methanol extract of Uvaria chamae leaves contains vitamin A (4871±79.21 I. U) and vitamin C (1.72±0.02%) while the root extract contains vitamin A (673.28±0.00I. U) and vitamin C (1.66±0.01%). Both extracts had equal contents of vitamin E (8.83±0.04 mg/100g). The leaf extract scavenged 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in a concentration dependent manner with the correlation coefficient (R2) of 0.839 and effective concentration (EC50) of 31.19 µg/ml, while the root extract scavenged DPPH with R2 of0.778 and EC50 of 14.00 µg/ml. The leaf and root extracts scavenged superoxide radical and hydroxyl radical with EC50 of 5.93 µg/ml and 719.45 µg/ml; 107.89 µg/ml and 912.01 µg/ml respectively compared to the EC50 of ascorbic standard (30.27 µg/ml) and EC50 of vitamin E standard (106.66µg/ml) respectively. The leaf extract showed significantly higher (p<0.05) anti radical power (ARP) of superoxide (0.17) compared to the root extract (0.0014) and the root extract showed significantly higher (p<0.05) ARP of DPPH (0.071) compared to the leaf extract (0.032).Conclusion: The leaves and roots of Uvaria chamae are rich in natural antioxidants that can be exploited in the treatment of diseases related to oxidative stress.

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