Stem Cell Characters in Primary and Metastatic Tumour Establishment

It is very important to understand the onset and growth pattern of breast primary tumours as well as their metastatic dissemination. In most cases, it is the metastatic disease that ultimately kills the patient. There is increasing evidence that cancer stem cells are closely linked to the progression of the metastatic tumour. Here, we investigate stem cell seeding to an avascular tumour site using an agent-based stochastic model of breast cancer metastatic seeding. The model includes several important cellular features such as stem cell symmetric and asymmetric division, migration, cellular quiescence, senescence, apoptosis and cell division cycles. It also includes external features such as stem cell seeding frequency and location. Using this model, we find that cell seeding rate and location are important features for tumour growth. We also define conditions in which the tumour growth exhibits decremented and exponential growth patterns. Overall, we find that seeding, senescence and division limit affect not only the number of stem cells, but also their spatial and temporal distribution.

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Author response for "Inhibition of neural stem cell aging through the transient induction of reprogramming factors"

10.1002/cne.24967/v2/response1 ◽

2020 ◽

Author(s):

Min Ji Han ◽

Won Ji Lee ◽

Joonhyuk Choi ◽

Yean Ju Hong ◽

Sang Jun Uhm ◽

...

Keyword(s):

Stem Cell ◽

Neural Stem Cell ◽

Author Response ◽

Cell Aging ◽

Stem Cell Aging ◽

Reprogramming Factors

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Decision letter for "Inhibition of neural stem cell aging through the transient induction of reprogramming factors"

10.1002/cne.24967/v2/decision1 ◽

2020 ◽

Keyword(s):

Stem Cell ◽

Neural Stem Cell ◽

Cell Aging ◽

Stem Cell Aging ◽

Reprogramming Factors

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The Response of Testes Cells to Low Level, Single dose Helium Particle Irradiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053929 ◽

1982 ◽

Vol 40 ◽

pp. 252-253

Author(s):

D.E. Philpott ◽

W. Sapp ◽

C. Williams ◽

J. Stevenson ◽

S. Black ◽

...

Keyword(s):

Stem Cell ◽

Single Dose ◽

B Cell ◽

Cell Survival ◽

Spermatogonial Stem Cell ◽

Low Doses ◽

Particle Irradiation ◽

Stem Cell Survival ◽

Irradiation Injury ◽

Radio Sensitivity

Spermatogonial stem-cell survival after irradiation injury has been studied in rodents by histological counts of surviving cells. Many studies, including previous work from our laboratory, show that the spermatogonial population demonstrates a heterogeneous response to irradiation. The spermatogonia increase in radio-sensitivity as differentiation proceeds through the sequence As - Apr - A1 - A2 - A3 - A4 - In - B. The stem (As) cell is the most resistant and the B cell is the most sensitive. The purpose of this work is to investigate the response of spermatogonial cell to low doses (less than 10 0 rads) of helium particle irradiation.

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The Response of Testis Cells to Low Dose X-Irradiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099155 ◽

1981 ◽

Vol 39 ◽

pp. 542-543

Author(s):

D. E. Philpott ◽

W. Sapp ◽

C. Williams ◽

Joann Stevenson ◽

S. Black

Keyword(s):

Electron Microscopy ◽

Stem Cell ◽

Light Microscopy ◽

Dose Level ◽

Cross Sections ◽

Low Dose ◽

Light And Electron Microscopy ◽

Cellular Responses ◽

Post Irradiation ◽

X Irradiation

The response of spermatogonial cells to X-irradiation is well documented. It has been shown that there is a radiation resistent stem cell (As) which, after irradiation, replenishes the seminiferous epithelium. Most investigations in this area have dealt with radiation dosages of 100R or more. This study was undertaken to observe cellular responses at doses less than 100R of X-irradiation utilizing a system in which the tissue can be used for light and electron microscopy.Brown B6D2F1 mice aged 16 weeks were exposed to X-irradiation (225KeV; 15mA; filter 0.35 Cu; 50-60 R/min). Four mice were irradiated at each dose level between 1 and 100 rads. Testes were removed 3 days post-irradiation, fixed, and embedded. Sections were cut at 2 microns for light microscopy. After staining, surviving spermatogonia were identified and counted in tubule cross sections. The surviving fraction of spermatogonia compared to control, S/S0, was plotted against dose to give the curve shown in Fig. 1.

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The early development of antenna and antennal sensilla in the noctuid moth, Agrotis segetum (Insecta: Lepidoptera)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160066 ◽

1990 ◽

Vol 48 (3) ◽

pp. 502-503

Author(s):

Eric Hallberg ◽

Lina Hansén

Keyword(s):

Cell Death ◽

Stem Cell ◽

Early Development ◽

Larval Stage ◽

Pupal Stage ◽

Agrotis Segetum ◽

Antennal Sensilla ◽

Noctuid Moth ◽

Standard Procedures

The antennal rudiments in lepidopterous insects are present as disks during the larval stage. The tubular double-walled antennal disk is present beneath the larval antenna, and its inner layer gives rise to the adult antenna during the pupal stage. The sensilla develop from a cluster of cells that are derived from one stem cell, which gives rise to both sensory and enveloping cells. During the morphogenesis of the sensillum these cells undergo major transformations, including cell death. In the moth Agrotis segetum the pupal stage lasts about 14 days (temperature, 25°C). The antennae, clearly seen from the exterior, were dissected and fixed according to standard procedures (3 % glutaraldehyde in 0.15 M cacaodylate buffer, followed by 1 % osmiumtetroxide in the same buffer). Pupae from day 1 to day 8, of both sexes were studied.

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