The Role of Trehalose Metabolism in Chloroplast Development and Leaf Senescence

Leaf senescence is the terminal stage in the development of perennial plants. Massive physiological changes occur that lead to the shut down of photosynthesis and a cessation of growth. Leaf senescence involves the selective destruction of the chloroplast as the site of photosynthesis. Here, we show that 13-lipoxygenase (13-LOX) accomplishes a key role in the destruction of chloroplasts in senescing plants and propose a critical role of its NH2-terminal chloroplast transit peptide. The 13-LOX enzyme identified here accumulated in the plastid envelope and catalyzed the dioxygenation of unsaturated membrane fatty acids, leading to a selective destruction of the chloroplast and the release of stromal constituents. Because 13-LOX pathway products comprise compounds involved in insect deterrence and pathogen defense (volatile aldehydes and oxylipins), a mechanism of unmolested nitrogen and carbon relocation is suggested that occurs from leaves to seeds and roots during fall.

Download Full-text

Role of Histones During Leaf Senescence

Senescence Signalling and Control in Plants ◽

10.1016/b978-0-12-813187-9.00011-1 ◽

2019 ◽

pp. 187-197 ◽

Cited By ~ 1

Author(s):

Bhubaneswar Pradhan ◽

Krishna Kumar Jangid ◽

Maryam Sarwat ◽

Sujit Kumar Bishi

Keyword(s):

Leaf Senescence

Download Full-text

Role of spermidine in the stabilization of the apoprotein of the light-harvesting chlorophyll a/b-protein complex of photosystem II during leaf senescence process

Acta Physiologiae Plantarum ◽

10.1007/s11738-999-0066-0 ◽

1999 ◽

Vol 21 (2) ◽

pp. 127-132 ◽

Cited By ~ 34

Author(s):

Jolanta Legocka ◽

Irena Zajchert

Keyword(s):

Photosystem Ii ◽

Chlorophyll A ◽

Leaf Senescence ◽

Protein Complex ◽

Light Harvesting

Download Full-text

Role of Arabidopsis CHL27 Protein for Photosynthesis, Chloroplast Development and Gene Expression Profiling

Plant and Cell Physiology ◽

10.1093/pcp/pcn111 ◽

2008 ◽

Vol 49 (9) ◽

pp. 1350-1363 ◽

Cited By ~ 41

Author(s):

Woo Young Bang ◽

In Sil Jeong ◽

Dae Won Kim ◽

Chak Han Im ◽

Chen Ji ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Expression Profiling ◽

Chloroplast Development

Download Full-text

Changes of the Polypeptide Composition in Thylakoid Membranes during Differentiation

Zeitschrift für Naturforschung C ◽

10.1515/znc-1990-3-419 ◽

1990 ◽

Vol 45 (3-4) ◽

pp. 253-257 ◽

Cited By ~ 6

Author(s):

Magdolna Droppa ◽

Jiri Masojidek ◽

Gábor Horváth

Keyword(s):

Molecular Weight ◽

Chloroplast Development ◽

Early Stage ◽

Relative Concentration ◽

Thylakoid Membranes ◽

Low Molecular Weight ◽

Polypeptide Composition ◽

Lhc Ii

Changes in membrane polypeptide composition during greening of etiolated maize were investigated to confirm the existence of the developmental polypeptides of 12 - 15 kDa described recently in virescent soybean mutant [M. Droppa, M. L. Ghirardi, G. Horváth, and A. Melis, Biochim. Biophys. Acta 932, 138 - 145 (1988)]. These low molecular weight polypeptides were the most abundant proteins at the early stage of greening, but were largely absent from fully developed thylakoids. During greening the relative concentration of the 12-15 kDa polypeptides were inversely proportional to that of LHC II, suggesting a role of these polypeptides in the assembly of the LHC II and/or chloroplast development.

Download Full-text

Characterization of Somatic Embryogenesis Receptor-Like Kinase 4 as a Negative Regulator of Leaf Senescence in Arabidopsis

Cells ◽

10.3390/cells8010050 ◽

2019 ◽

Vol 8 (1) ◽

pp. 50 ◽

Cited By ~ 6

Author(s):

Xiaoxu Li ◽

Salman Ahmad ◽

Akhtar Ali ◽

Cun Guo ◽

Hong Li ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Leaf Senescence ◽

Stress Responses ◽

Negative Regulator ◽

Knockout Mutant ◽

Surface Receptors ◽

Extracellular Signals ◽

Early Leaf Senescence

Leaf senescence is a genetically controlled process that involves the perception of extracellular signals and signal transduction. The receptor-like protein kinases (RLKs) are known to act as an important class of cell surface receptors and are involved in multiple biological processes such as development and stress responses. The functions of a number of RLK members have been characterized in Arabidopsis and other plant species, but only a limited number of RLK proteins have been reported to be associated with leaf senescence. In the present study, we have characterized the role of the somatic embryogenesis receptor kinase 4 (SERK4) gene in leaf senescence. The expression of SERK4 was up-regulated during leaf senescence and by several abiotic stress treatments in Arabidopsis. The serk4-1 knockout mutant was found to display a significant early leaf senescence phenotype. Furthermore, the results of overexpression analysis and complementary analysis supported the idea that SERK4 acts as a negative regulator in the process of leaf senescence.

Download Full-text

A WRKY transcription factor, TaWRKY42-B, facilitates initiation of leaf senescence by promoting jasmonic acid biosynthesis

BMC Plant Biology ◽

10.1186/s12870-020-02650-7 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Ming-Ming Zhao ◽

Xiao-Wen Zhang ◽

Yong-Wei Liu ◽

Ke Li ◽

Qi Tan ◽

...

Keyword(s):

Leaf Senescence ◽

Expression Profiles ◽

Functional Characterization ◽

Wrky Transcription Factor ◽

Positive Role ◽

Environmental Signals ◽

Source To Sink ◽

Age Dependent ◽

Functional Investigation

Abstract Background Leaf senescence comprises numerous cooperative events, integrates environmental signals with age-dependent developmental cues, and coordinates the multifaceted deterioration and source-to-sink allocation of nutrients. In crops, leaf senescence has long been regarded as an essential developmental stage for productivity and quality, whereas functional characterization of candidate genes involved in the regulation of leaf senescence has, thus far, been limited in wheat. Results In this study, we analyzed the expression profiles of 97 WRKY transcription factors (TFs) throughout the progression of leaf senescence in wheat and subsequently isolated a potential regulator of leaf senescence, TaWRKY42-B, for further functional investigation. By phenotypic and physiological analyses in TaWRKY42-B-overexpressing Arabidopsis plants and TaWRKY42-B-silenced wheat plants, we confirmed the positive role of TaWRKY42-B in the initiation of developmental and dark-induced leaf senescence. Furthermore, our results revealed that TaWRKY42-B promotes leaf senescence mainly by interacting with a JA biosynthesis gene, AtLOX3, and its ortholog, TaLOX3, which consequently contributes to the accumulation of JA content. In the present study, we also demonstrated that TaWRKY42-B was functionally conserved with AtWRKY53 in the initiation of age-dependent leaf senescence. Conclusion Our results revealed a novel positive regulator of leaf senescence, TaWRKY42-B, which mediates JA-related leaf senescence via activation of JA biosynthesis and has the potential to be a target gene for molecular breeding in wheat.

Download Full-text