Stable Synthetic Affinity Ligands for Use in Protein Fractionation

1990 ◽  
pp. 265-274 ◽  
Author(s):  
Steven J. Burton ◽  
James C. Pearson ◽  
David L. Stewart
Keyword(s):  
Protein Fractionation ◽  
Affinity Ligands

scholarly journals Comparative Assessment of Tubular Ceramic, Spiral Wound, and Hollow Fiber Membrane Microfiltration Module Systems for Milk Protein Fractionation

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 692
Author(s):  
Roland Schopf ◽  
Florian Schmidt ◽  
Johanna Linner ◽  
Ulrich Kulozik
Keyword(s):  
Hollow Fiber ◽  
Milk Protein ◽  
Hollow Fiber Membrane ◽  
Comparative Assessment ◽  
Transmission Factor ◽  
Transmembrane Pressure ◽  
Protein Fractionation ◽  
Flow Conditions ◽  
Local Pressure ◽  
Spiral Wound

The fractionation efficiency of hollow fiber membranes (HFM) for milk protein fractionation was compared to ceramic tubular membranes (CTM) and spiral wound membranes (SWM). HFM combine the features of high membrane packing density of SWM and the more defined flow conditions and better control of membrane fouling in the open flow channel cross-sections of CTM. The aim was to comparatively analyze the effect of variations in local pressure and flow conditions while using single industrially sized standard modules with similar dimensions and module footprints (module diameter and length). The comparative assessment with varied transmembrane pressure was first applied for a constant feed volume flow rate of 20 m3 h−1 and, secondly, with the same axial pressure drop along the modules of 1.3 bar m−1, similar to commonly applied crossflow velocity and wall shear stress conditions at the industrial level. Flux, transmission factor of proteins (whey proteins and serum caseins), and specific protein mass flow per area membrane and per volume of module installed were determined as the evaluation criteria. The casein-to-whey protein ratios were calculated as a measure for protein fractionation effect. Results obtained show that HFM, which so far are under-represented as standard module types in industrial dairy applications, appear to be a competitive alternative to SWM and CTM for milk protein fractionation.

scholarly journals Protein L—More Than Just an Affinity Ligand

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 874
Author(s):  
Stefan Kittler ◽  
Mihail Besleaga ◽  
Julian Ebner ◽  
Oliver Spadiut
Keyword(s):  
Antibody Fragments ◽  
Downstream Process ◽  
Affinity Ligands ◽  
The Past ◽  
Affinity Ligand ◽  
Recombinant Production ◽  
Production Processes ◽  
Monitoring Tools ◽  
Platform Technology ◽  
Analytical Tools

In the past 30 years, highly specific drugs, known as antibodies, have conquered the biopharmaceutical market. In addition to monoclonal antibodies (mAbs), antibody fragments are successfully applied. However, recombinant production faces challenges. Process analytical tools for monitoring and controlling production processes are scarce and time-intensive. In the downstream process (DSP), affinity ligands are established as the primary and most important step, while the application of other methods is challenging. The use of these affinity ligands as monitoring tools would enable a platform technology to monitor process steps in the USP and DSP. In this review, we highlight the current applications of affinity ligands (proteins A, G, and L) and discuss further applications as process analytical tools.

Design and synthesis of affinity ligands and relation of their structure with adsorption of proteins

2011 ◽  
Vol 34 (22) ◽  
pp. 3145-3150 ◽  
Author(s):  
Long Ye ◽  
Aizhang Xu ◽  
Chao Cheng ◽  
Li Zhang ◽  
Chenxi Huo ◽  
...  
Keyword(s):  
Affinity Ligands ◽  
Design And Synthesis

Infectious bronchitis virus: Identification of Gallus gallus APN high-affinity ligands with antiviral effects

2021 ◽  
Vol 186 ◽  
pp. 104998
Author(s):  
Xiaoqi Sun ◽  
Lanlan Li ◽  
Long Pan ◽  
Zheng Wang ◽  
Huijie Chen ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Gallus Gallus ◽  
Affinity Ligands ◽  
Virus Identification ◽  
Infectious Bronchitis ◽  
High Affinity ◽  
Antiviral Effects

Purification of bacterial virulence factor pertactin using high affinity ligands

2020 ◽  
Vol 164 ◽  
pp. 107760
Author(s):  
Umatheny Umatheva ◽  
Braden Sweeting ◽  
Léo Sauvaget ◽  
Nerissa Dela Rosa ◽  
John Riley ◽  
...  
Keyword(s):  
Virulence Factor ◽  
Bacterial Virulence ◽  
Affinity Ligands ◽  
High Affinity ◽  
Bacterial Virulence Factor
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