Crude protein fractionation, in situ ruminal degradability and FTIR protein molecular structures of different cultivars within barley, corn and sorghum cereal grains

2021 ◽  
Vol 275 ◽  
pp. 114855
Author(s):  
Hojjat Gholizadeh ◽  
Abbas A. Naserian ◽  
Mojtaba Yari ◽  
Arjan Jonker ◽  
Peiqiang Yu
Keyword(s):  
Crude Protein ◽  
Molecular Structures ◽  
Cereal Grains ◽  
Protein Fractionation ◽  
Ruminal Degradability

Factors influencing composition and ruminal degradability of barley straw

1999 ◽  
Vol 79 (3) ◽  
pp. 343-351 ◽  
Author(s):  
G. W. Mathison ◽  
R. Soofi-Siawash ◽  
E. K. Okine ◽  
J. Helm ◽  
P. Juskiw
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Neutral Detergent Fiber ◽  
Barley Straw ◽  
Medium Length ◽  
Key Words Barley ◽  
Ruminant Animals ◽  
Ruminal Degradability ◽  
Barley Genotypes

Chemical composition and in situ ruminal degradability characteristics for 25 and 40 genotypes of barley straw were measured for crops grown in 1994 and 1995, respectively. Straw from semidwarf genotypes contained more crude protein and less neutral detergent fiber (NDF) than that from genotypes with medium straw length and effective ruminal degradability was 9% higher (P < 0.01). Straw from two-row genotypes contained 19% more crude protein (4.4 vs. 3.7%) and 3% less NDF (75.2 vs. 77.6%) than that from six-row genotypes and effective ruminal degradability was 6% higher (P < 0.01) when genotypes with medium-length straw only were considered. Genotypes with smooth awns had higher rates of degradation but contained less of the slowly degraded fraction than straw with rough awns when straws of medium straw length were compared (P < 0.05). Straw from hulless genotypes contained more (P = 0.006) NDF than that from covered varieties. Both ambient and soil temperature were positively related (P < 0.05) to effective ruminal degradability of straw, possibly because of associated greater leafiness when temperatures were higher and there was more light. It was concluded that it is possible to select barley genotypes that have superior straw nutritive value for ruminant animals. Key words: Barley, straw, degradability, cattle, genotype, environment

scholarly journals DEGRADAÇÃO RUMINAL DA MATÉRIA SECA E DE PROTEÍNA BRUTA DE VOLUMOSOS PARA BOVINOS Ruminal degradability of dry matter and crude protein of roughages for cattle

2012 ◽  
Vol 10 (3) ◽  
pp. 285 ◽  
Author(s):  
Rafael Henrique De Tonissi e Buschinelli ◽  
Rita De Cássia Menchon Tramontini ◽  
Sérgio Tosi Cardim ◽  
Gabriel Daltoé de Almeida ◽  
Juliane Ribeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Panicum Maximum ◽  
Brachiaria Brizantha ◽  
Ruminal Degradability

O objetivo deste trabalho foi avaliar a degradabilidade ruminal da matéria seca e proteína bruta por meio da técnica in situ dos capins Brachiaria brizantha e Panicum maximum cortados aos 60 dias de idade, das silagens de milho, sorgo e milheto, dos fenos de coast cross, guandu e soja perene e do farelo de soja. Foram utilizados três novilhos com peso médio de 300 kg, fistulados e providos de cânulas ruminais. Os volumosos foram incubados em ordem decrescente de 72, 48, 24, 18, 12, 6, 3 e 0h, e o farelo de soja incubado nos tempos 48, 24, 18, 12, 6, 3 e 0h. Os resíduos dos sacos foram analisados quanto aos teores de matéria seca e proteína bruta. O farelo de soja apresentou alta degradabilidade efetiva para MS em função das frações solúveis (32,85%) e fração potencialmente degradável (65,75%). A silagem de sorgo apresentou elevada fração solúvel e potencialmente degradável se comparada às demais silagens. O feno de soja perene apresentou a maior degradabilidade de MS (80,42%), entre os volumosos avaliados. Para a proteína, destacam-se a baixa degradabilidade apresentada pelo feno de guandu (36,91%) e os demais volumosos apresentaram degradabilidade variando entre 64,50 a 74,56%.

scholarly journals Characterization of proteins in feeds according to the CNCPS and comparison to in situ parameters

2014 ◽  
Vol 59 (No. 6) ◽  
pp. 288-295 ◽  
Author(s):  
M. Chrenková ◽  
Z. Čerešňáková ◽  
M.R. Weisbjerg ◽  
Z. Formelová ◽  
M. Poláčiková ◽  
...  
Keyword(s):  
Crude Protein ◽  
Total N ◽  
In Situ Method ◽  
Legume Seeds ◽  
Ruminal Degradability ◽  
Feed Protein ◽  
True Protein ◽  
By Products

Several methods were used for the evaluation of feed protein for rumen degradability and intestinal digestibility of rumen undegraded protein (RUP). The aim of this work was to explore the possibility of using the Cornell method of predicting the fraction RUP. An in situ method was adopted for estimation of degradability parameters (a, b, c) and effective degradable crude protein (EDCP), and five nitrogen fractions (A, B<sub>1</sub>, B<sub>2</sub>, B<sub>3</sub>, and C) were determined according to the Cornell Net Carbohydrate and Protein System (CNCPS). Fifty-one feedstuffs &ndash; 13 cereals grains, 5 legume seeds, 3 oilseeds, 11 oilseed by-products, 4 distillers dried grains with solubles (DDGS), and 15 silages (maize, lucerne, grass, and grass-clover) &ndash; were used in this work. The examined feedstuffs varied widely in nutrient composition. Fraction B<sub>1 </sub>(soluble true protein) in forages was small (2.5&ndash;5.7% of total N), but it varied to a large extent in concentrates (DDGS 0.9&ndash;1.2, legumes 46.5&ndash;63.7, oilseeds meal 17.1&ndash;51.8% of total N). Fraction B<sub>2</sub> represented a large proportion of the total protein in oil seed meals (44.3&ndash;82.6% of total N) and in DDGS (55.8&ndash;77.8% of total N), too. Fraction B<sub>3</sub> was relatively small (less than 10% of total N) in all feedstuffs and declined with increasing acid detergent insoluble nitrogen (ADIN) concentration. The concentration of ADIN fraction in feeds affected ruminal degradability. Lucerne silage, with a high content of ADIN (30.9% of total N), had a low effective crude protein (CP) degradability (57%). Correlation between EDCP and fraction A was r = 0.76. A weaker correlation (r = 0.67) was found between in situ parameter &ldquo;b&rdquo; and fractions B<sub>2 </sub>and B<sub>3 </sub>(r = 0.59), respectively. The results show that much more samples of all feed types should be analyzed to obtain results allowing a more exact prediction of CP degradability and RUP. &nbsp;

Degradability chracteristics of dry matter and crude protein of Iranian forages in ruminant

2002 ◽  
Vol 2002 ◽  
pp. 144-144
Author(s):  
A. Taghizadeh ◽  
J. shoja ◽  
G. Moghaddam ◽  
H. Jhonmohammadi ◽  
P. Yasan
Keyword(s):  
North America ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Dairy Farms ◽  
Evaluation Systems ◽  
Ruminal Degradability ◽  
Sheep Rumen ◽  
Male Sheep

Values for crude protein (CP) and ruminally undegradable CP content are now required in feed evaluation systems currently used in North America (NRC,1989) and other organizations (Ørskov,1992).The objectives of the present study were to assess in situ ruminal degradability, fractional rates of digestion and the effective degradability of the dry matter (DM) and crude protein (CP) of alfalfa and red clover hays, selected randomly from dairy farms of Tabriz region in Iran on the male sheep rumen.

scholarly journals Digestión de materia seca, proteína cruda y aminoácidos de la dieta de vacas lecheras

2017 ◽  
Vol 28 (2) ◽  
pp. 341
Author(s):  
Monica Duque Quintero ◽  
Ricardo Rosero Noguera ◽  
Marta Olivera Ángel
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Crude Protein ◽  
Dry Matter ◽  
Descriptive Statistics ◽  
Passage Rate ◽  
Ruminal Degradability ◽  
Kikuyu Grass

The aim of this study was to determine the digestion of dry matter (MS), crude protein (PC) and amino acids (AA) in dairy cows. Two cannulated cows were used for the determination of passage rate (Kp), in situ ruminal degradability (DR) and intestinal digestibility (DI) by abomasal catheter. The data to calculate Kp was analyzed with NLIN procedure by SAS, and descriptive statistics for DR and DI of MS, PC and AA from Kikuyu grass (Pennisetum clandestinum), a commercial supplement and two sources of rumen-protected AA. The study showed a Kp and a ruminal retention time of 0.036 h-1 and 27.4 h. The highest values from DR of MS and CP were from Kikuyu grass (69.0 and 61.8%) and concentrate (84.7 and 77.2%), followed by MetP (60.2 and 66.7%) and LysP (6.72 and 11.4%). The highest percentages of rumen indegradable amino acids (AADR) were from Kikuyu and concentrate, varying between 58.7 and 68% in forage, and 76.1 and 82.9% in the concentrate. The DR was 11.5 and 65.8% in LysP and MetP, respectively. The DI of AA (%AADR) varied between 42.3 and 77.4% for Kikuyu and 42.2 and 59.3% for concentrate. The values for the protected amino acids were 42.1 for LysP and 58.6 for MetP. 

scholarly journals In situ ruminal degradation of sallow tree leaves using different mathematical models

2021 ◽  
Vol 26 (3) ◽  
pp. e2170
Author(s):  
Valiollah Palangi
Keyword(s):  
Mathematical Models ◽  
Crude Protein ◽  
Dry Matter ◽  
Gompertz Model ◽  
Exponential Model ◽  
Salix Alba ◽  
Estimated Parameters ◽  
Ruminal Degradation ◽  
Ruminal Degradability

Objective. The objective of this study was to determine the in situ digestion parameters of sallow leaves (Salix Alba) as an alternative food, not commonly used in animal diets using mathematical models. Materials and methods. Four mathematical models were used to describe the ruminal disappearance of dry matter (DM) and crude protein (CP) of sallow leaves: non lagged exponential (Model I); lagged exponential (Model II); Gompertz (Model III), and generalized Mitscherlich (Model IV). Results. Results of DM and CP degradability characteristics showed that all the models fitted well (r2>0.98) to the disappearance data. There were minor differences between the models in terms of statistical evaluations. However, the models differed in the estimated parameters depending on the model’s nature and structure and the parameters included. Conclusions. Given that some models estimated negative values for the studied parameters, they were not biologically acceptable. For this reason, the only model I can be used for estimating the degradability of DM and CP of sallow leaves. In terms of effective degradability (ED) of DM, model III was not used in the calculations because of calculating negative values for part A. Sallow leaves constitute the largest possible proportion of the ruminal degradability fractions, and effective degradability can be used as a forage source in ruminant feeds.

scholarly journals In situ ruminal degradability and in vitro intestinal digestibility of crude protein from some minor legume species

2013 ◽  
Vol 12 (17) ◽  
pp. 2293-2302 ◽  
Author(s):  
Seifdavati Jamal ◽  
Taghizadeh Akbar ◽  
Janmohammadi Hosein ◽  
Abbas Rafat Seyd ◽  
Alijani Sadegh
Keyword(s):  
Crude Protein ◽  
Legume Species ◽  
Ruminal Degradability ◽  
Intestinal Digestibility

scholarly journals Degradabilidad in situ de la materia seca, proteína y fibra del forraje de morera (Morus alba).

2006 ◽  
Vol 12 (1) ◽  
pp. 79
Author(s):  
Carlos Boschini
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Soluble Fraction ◽  
Neutral Detergent Fiber ◽  
Whole Plant ◽  
Degradation Rates ◽  
Entire Plant ◽  
Ruminal Degradation ◽  
Ruminal Degradability

The purpose of this study was to determine the ruminal degradability of dry matter, crude protein, neutral detergent fiber and their fractions, contained in regrowth of mulberry at 56, 84 and 112 days. The degradation of leaves, stems and the whole plant was calculated. The potential degradability of these three components diminished as the regrowth aged. The soluble fraction of the dry matter was reduced from 29% to 25% in the leaves and from 27% to 19% in the stems between the 56th and the112th day. The degradable fraction in the stem remained constant (61-63%) on the three measuring days, with ruminal degradation levels of 14%, 10% and 9%/h respectively. In the stem the degradable fraction diminished from 42% to 30% between the 56th and 112th day, with degradation speeds of 8.4%, 6.7 % and 5.3%/h. The soluble crude protein diminished with age from 41% to 33% in the entire plant and the degradable fraction remained constant between 49% and 51%. The degradation rate was higher than 9.4%/h. The neutral detergent fiber had a degradable fraction of 71%, 62% y 43% on the 56th, 84th and 112th day, with degradation rates of 13%, 10% y 9%/h respectively. The degradation of the neutral detergent fiber began in the rumen with a lag time of 1.5 to 3.2 hours.

scholarly journals Bromatological composition and ruminal degradability of Xaraés palisade grass under grazing in integrated systems

2021 ◽  
Vol 43 ◽  
pp. e53004
Author(s):  
Francyelle Ruana Faria da Silva ◽  
Ana Karina Dias Salman ◽  
Pedro Gomes da Cruz ◽  
Marlos Oliveira Porto ◽  
Jucilene Cavali ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Brachiaria Brizantha ◽  
Passage Rate ◽  
Crown Cover ◽  
Ruminal Degradability ◽  
Urochloa Brizantha

To evaluate the bromatological composition and ruminal degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of Xaraés palisade grass (Urochloa brizantha ‘Xaraes’ syn Brachiaria brizantha) under grazing in integrated crop, livestock (ICL), and forest (ICLF) systems, we conducted an in situ degradability trial in randomized blocks with three non-lactating 3/4 Gyr × 1/4 Holstein cows, provided with ruminal cannula. The management of Xaraés palisade grass was similar in both systems, differing only regarding shading in the ICLF system provided by eucalyptus trees (average 65% crown cover). Grass samples were incubated for 0, 3, 6, 9, 12, 24, 36, 48, 72, and 96 hours. Considering the passage rate 2% h-1, the Xaraés palisade grass of ICL system had greater NDF effective degradability in relation to ICLF (46.38% vs 44.98%). However, the palisade grass CP potential degradability was greater in the ICLF than in the ICL system (68.92% vs. 65.40%). The presence of trees in the pasture has effect on nutritional traits of the Xaraés palisade grass, increasing its protein content and degradability and reducing its fiber degradability.

Characteristics and in situ degradability of whole crop faba bean, pea, and soybean silages

2003 ◽  
Vol 83 (4) ◽  
pp. 793-799 ◽  
Author(s):  
A. F. Mustafa ◽  
P. Seguin
Keyword(s):  
Faba Bean ◽  
Crude Protein ◽  
Neutral Detergent Fiber ◽  
Rapid Decline ◽  
Protein Nitrogen ◽  
Protein Levels ◽  
Glycine Max L ◽  
Ruminal Degradability ◽  
True Protein

The objective of this study was to determine the ensiling characteristics and ruminal nutrient degradabilities of faba bean (FS, Vicia faba L.), soybean (SS, Glycine max (L.) Merr.) and pea (PS, Pisum sativum L.) silages. Forages were harvested between the beginning- and full-seed stages and ensiled in mini-silos for 0, 2, 4, 16, and 45 d. Two lactating Holstein cows fitted with ruminal fistulae were used to determine ruminal nutrient degradabilities. Results showed that all silages went through a rapid fermentation as indicated by rapid decline in pH and sharp increase in lactic acid concentration within the first 2 d of ensiling. Extensive proteolysis took place between day 0 and day 4 post-ensiling as indicated by a significant reduction in true protein (TP) and a significant increase in non-protein nitrogen (NPN). During the 45 d of ensiling, TP decreased (P < 0.05) by 26, 31, and 45% while NPN increased (P < 0.05) by 82, 54, and 47% for FS, SS, and PS, respectively. Chemical analysis showed that the three 45-d silages contained a similar level of neutral detergent fiber (NDF, average 421 g kg-1). Crude protein (CP) was highest (P < 0.05) for FS, intermediate for SS, and lowest for PS. Distribution of protein fractions showed that soluble protein and NPN were highest (P < 0.05) for PS, intermediate for SS, and lowest for FS. Neutral (NDICP) and acid (ADICP) detergent insoluble protein levels followed an opposite order to that observed for soluble crude protein (SCP) and NPN. Results of the in situ study showed that SS and PS had a similar ruminal degradability of dry matter (average 68.8%) and CP (82.9%), which were higher (P < 0.05) than those of FS (66.2 and 79.4%, respectively). However, no differences in ruminal degradability of NDF (average 34.7%) were observed between the three legume silages. Key words: Silage, ensiling, forage quality, nutrient degradability

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