Comparative Studies on Growth and Volatile Oil Contents of Some Induced Mutants of Origanum Majorana

'Marjoram,' Origanum majorana L., a culinary aromatic medicinal herb is known to possess various therapeutic properties. We evaluated the antiulcerogenic activity of the ethanol extract in hypothermic restraint stress-, indomethacin-, necrotizing agents- (80% ethanol, 25% NaCl and 0.2 M NaOH ) induced ulcers and basal gastric acid secretion using pylorus ligated Shay rat-model. Marjoram at doses of 250 and 500 mg/kg of body weight, significantly decreased the incidence of ulcers, basal gastric secretion and acid output. Furthermore, the extract replenished the ethanol-induced depleted gastric wall mucus and nonprotein sulfhydryls (NP-SH) contents and significantly lowered the increase in the concentration of malondialdehyde (MDA). Ulcer preventing potential was further confirmed by histopathological assessment. An acute toxicity test showed a large margin of safety of the extract in mice. The phytochemical screening of aerial parts of marjoram revealed the presence of volatile oil, flavonoids, tannins, sterols and/or triterpenes.

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Studies on production techniques of some herb plants: I Effect of Agryl P17 mulching on herb yield and volatile oils of basil (Ocimum basilicum L.) and marjoram (Origanum majorana L.)

Agricultural and Food Science ◽

10.23986/afsci.72243 ◽

1987 ◽

Vol 59 (1) ◽

pp. 31-36

Author(s):

S. Hälvä

Keyword(s):

Oil Content ◽

Total Content ◽

Volatile Oil ◽

Ocimum Basilicum ◽

The North ◽

Production Techniques ◽

Herbal Plants ◽

Cold Sensitive ◽

Total Oil ◽

Origanum Majorana

Agryl P17 fiber-mulching of cold-sensitive herbal plants, basil (Ocimum basilicum L.) and marjoram (Origanum majorana L.), were studied at three locations in Finland (1984—1985). The growing sites were Helsinki (60° 14' N), Sahalahti (61° 28' N), and Inari (69° 04' N) for both species in 1984, and Helsinki for marjoram in 1985. Agryl P17 mulching increased basil yield at all locations. The uncovered basil yielded approximately 54 kg/100 m2 and when grown under the mulch, more than three fold, 191 kg/ 100m2. In the north (Inari), however, basil and marjoram did not give practically any yield. Marjoram did not benefit from mulching either in the south: the yield was 96 kg without and 80 kg/100m2 with mulching. The vegetation under the mulch was severely affected by fungus diseases. The volatile oil content in the dried basil herb ranged from 0.46 to 0.93 %. There were no significant differences in the total oil content whether basil was grown with or without Agryl P17. The oil content in marjoram ranged from 1.94 to 2.55 % the total content being significantly higher when grown under the cover.

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Volatile oil production, proline, protein and DNA profile in three medicinal plants exposed to short-term drought stress

Bangladesh Journal of Botany ◽

10.3329/bjb.v47i4.47398 ◽

2018 ◽

Vol 47 (4) ◽

pp. 975-983

Author(s):

Amal Fadl Abdelkader ◽

Hala Ragab Nosair

Keyword(s):

Drought Stress ◽

Medicinal Plants ◽

Genomic Dna ◽

Volatile Oil ◽

Antioxidant Property ◽

Short Term ◽

Dna Profile ◽

Phytochemical Compounds ◽

Molecular Aspects ◽

Origanum Majorana

This present work deals with monitoring some molecular aspects standing beyond the varied production of volatile oil in three medicinal plants (Ocimum basilicum, B, Mentha longifolia, M and Origanum majorana, O) after ten days of exposure to drought stress by using 250 mM mannitol as a drought inducer. The results showed that proline has increased in all drought-stressed plants as compared to the control. Four protein bands (210, 70, 63 and 18 kDa) have disappeared in stressed B-plant, in addition to low molecular weight newly expressed protein bands (16-78 kDa). The protein polymorphism has varied among the species and ranged from 16.66 to 54.54%. RAPD-DNA technique indicated high similarity of genomic-DNA in stressed and unstressed plants. GC-MS screened intensive fluctuations within the pool of phytochemical compounds. The genomic DNA structure was generally stable under drought stress despite of new proteins expression which mediated the induction of chemical compounds with antioxidant property to cope with the drought stress.

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