Prediction of Protein Nutritive Value of Cereal-Legume Blends Using Rat Bioassays and Amino Acid Scores

Author(s):  
F. W. Sosulski ◽  
G. Sarwar
Keyword(s):  
2018 ◽  
Vol 74 (10) ◽  
pp. 5971-2018 ◽  
Author(s):  
PRZEMYSŁAW KNYSZ ◽  
MICHAŁ GONDEK ◽  
RENATA PYZ-ŁUKASIK ◽  
MONIKA ZIOMEK ◽  
ŁUKASZ DROZD ◽  
...  

The aim of the study was to determine the variability in the chemical composition and nutritive value parameters of smoked and unsmoked short-ripened rennet cheeses and unsmoked long-ripened rennet cheeses produced by traditional methods. The raw material for the production of short-ripened cheeses was pasteurized cows’ milk obtained from a dairy, whereas the long-ripened cheeses were manufactured from raw cows’ milk obtained from the producer’s farm. All three varieties of cheese examined were produced with commercial dairy starter cultures. The material for the study was collected in winter, directly at the producers’ retail outlets in southern and eastern Poland. The basic chemical composition was determined according to the Polish Standards, whereas the amino acid profiles of proteins from the cheeses were determined by ion-exchange chromatography. The result analysis revealed significant differences between the different varieties of cheese in terms of their water content, ranging from 32.2% to 42.1%, as well as protein content, which varied from 25.6% to 31.6%. Fat levels ranged between 22.2% and 24%, whereas total ash content amounted to 5.1-5.8%. The significantly highest salt content was found in unsmoked short-ripened cheeses. In all three cheese varieties, the total exogenous amino acid content was comparable, ranging from 46.17 g to 47.36 g/100 g protein, and that of endogenous amino acids varied from 52 g to 53 g/100 g protein. The biological value of proteins was determined by calculating to the chemical score (CS), as described by Mitchell and Block, and the essential amino acid index (EAAI), as described by Oser. A comparison of the results with the standard chicken egg white proteins showed that the limiting amino acids for all varieties of cheese were methionine and cysteine. On the other hand, a comparison with the FAO/WHO-suggested pattern of amino acid requirements (1991) for all age groups over 1 year of age showed that the limiting amino acids were methionine and cysteine in smoked and unsmoked short-ripened cheeses, and treonine in long-ripened cheeses. Considering the chemical indices, such as CS and EAAI, it may be concluded that the traditional rennet cheeses produced in southern and eastern Poland have a favourable amino acid composition of proteins and a high nutritive value..


2011 ◽  
Vol 75 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Anna Kotlarz ◽  
Agnieszka Sujak ◽  
Wacław Strobel ◽  
Wilhelm Grzesiak

Chemical Composition and Nutritive Value of Protein of the Pea Seeds - Effect of Harvesting Year and VarietyWe tested nutritional usability of pea seeds (Pisum sativumL.) at full maturity. Four white-flowering and 5 colour-flowering new Polish cultivars were compared. We determined proximate composition of ground seeds collected over 4 years period as well as amino acids, minerals (Ca, P, Na, K), tannins and fiber fractions (NDF, ADF, ADL). The seeds contained between 224 and 260 g·kg-1of crude protein which was rich in Lys (6.8±0.8 g) but poor in Met+Cys (2.0±0.2 g·16 g-1N). The amount of tannins (as tannic acid equivalent) in white-flowering cvs was 4.3±0.9 g and in colour-flowering - 7.4±2.2 g. The calculated protein nutritional values were compared against amino acid standards of human and animal nutrition and the whole egg protein. Effects of the cropping year and variety variance in respect of chemical composition of seeds were examined. Crude protein, crude oil, N-free extractives, tannins, K, amino acid content: Leu, Phe+Tyr and Ala content were influenced significantly by the cultivar, while the cropping year had a significant influence on dry matter, crude ash, crude protein, crude fibre, crude oil, N-free extractives ADF and ADL fiber fractions, content of P, Na and K, most of the amino acid levels and on nutritional values of the protein measured by means of CS and EAAI indices.


2019 ◽  
Author(s):  
◽  
Alaa Alaswad

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Soybean is one of the most important field crops grown in the United States. Commercial soybeans contain about 36 - 40% protein and 18 - 20% oil, by dry weight. Most of the soybean produced in the U.S. is utilized for oil production and animal feed. Even though soybean is a rich source of protein for livestock and humans, the nutritional quality of soybean can be further enhanced if the concentration of sulfur containing amino acids, methionine and cysteine, can be increased. Previously, our lab has employed transgenic approaches to increase the nutritive value of soybean by expressing heterologous sulfur-rich seed proteins and by manipulating the sulfur assimilatory pathway. Even though modest improvement in the nutritive value has been obtained by these approaches, till now no high yielding soybean cultivars with high protein and high sulfur amino acid content has been developed. Such cultivars will be of great value for the agricultural industry especially in animal feed. In this study, I have crossed a South Korean high protein soybean line (Lee5) with a transgenic soybean line with elevated sulfur amino acid content (CS) and developed soybean experimental lines that were advanced to F4:5 seeds. Theses experimental lines exhibit both high protein and high sulfur amino acid content. The protein content of their seeds has been increased 8 to 14% while their oil content has decreased 5 to 7% when compared with CS. Furthermore, I have successfully introduced the cytosolic isoform of O-acetylserine sulfhydrylase (OASS), the overexpressed transgene, from CS into these crosses. The activity of OASS has been increased 5 to 20-fold in experimental lines when compared to Lee5.


2005 ◽  
Vol 88 (3) ◽  
pp. 874-876 ◽  
Author(s):  
Paul J Moughan

Abstract The first objective in evaluating protein quality is to permit a ranking of proteins according to their potential nutritive value and to permit detection of changes in nutritive value due to processing and/or storage. The second objective is to permit prediction of the contribution a food protein, or mixture of food proteins, makes toward meeting nitrogen and amino acid requirements for growth or maintenance. Different approaches are used in meeting these distinct aims. The preferred current method to meet the second aim is the protein digestibility corrected amino acid score (PDCAAS). This article introduces the concept of PDCAAS and places it in the context of the series of papers published in this Special Guest Editor Section addressing aspects of dietary amino acid utilization.


1960 ◽  
Vol 71 (4) ◽  
pp. 361-365 ◽  
Author(s):  
P. B. Rama Rao ◽  
V. Chalam Metta ◽  
H. W. Norton ◽  
B. Connor Johnson

1988 ◽  
Vol 51 (11) ◽  
pp. 866-868
Author(s):  
M. L. FIELDS ◽  
A. AL-SHOSHAN ◽  
Y. POOSIRI

One-step fermentation involving two microorganisms inoculated at the same time and two-step fermentations involving two inoculations of different microbes at different times were used to enrich corn meal. Starch in corn meal was first hydrolyzed by amylases of B. stearothermophilus, E. fibuligera or A. oryzae followed by the growth of C. utilis. The combination of E. fibuligera and C. utilis produced a significant (P<0.05) increase in lysine, methionine, tryptophan. The relative nutritive value (%), which reflected the amino acid balance, increased significantly (P<0.05) with this sequence of microorganisms. Niacin, riboflavin, and thiamin increased significantly (P<0.05) when mixed cultures of A. oryzae and E. fibuligera in combination with C. utilis were employed. When E. fibuligera alone was grown, no significant change was observed in thiamin content but significant increases occurred in niacin and riboflavin. A. oryzae by itself produced significant (P<0.05) changes in niacin, riboflavin and thiamin.


2007 ◽  
Vol 2007 ◽  
pp. 84-84
Author(s):  
M. J. Hutchinson ◽  
M. E. E. McCann ◽  
V Beattie

The addition of ‘high quality’ complementary feedingstuffs to the diet of the post weaning pig can positively impact on growth and lean muscle deposition. The Maillard Reaction bonds amino acid and sugar molecules together, and is one of the major pathways in the chemical changes that occur in the cooking process. Cooking of feedingstuffs has been shown to improve the digestibility and nutritive value of a diet (Pickford et al, 1992). In this study, lysine (Lys), methionine (Met) and threonine (Thr) where chemically reacted with sugar molecules to give in vitro early Maillard Reaction Products (MRP). The aim of this study was to assess what effect the addition of a solution of these MRPs to a complementary feedingstuff (Matan XL) would have on overall diet digestibility and subsequent piglet performance.


1975 ◽  
Vol 23 (1) ◽  
pp. 24-26 ◽  
Author(s):  
Krishan C. Sikha ◽  
Rajinder P. Johari ◽  
Surinder K. Duggal ◽  
Ved P. Ahuja ◽  
A. Austin

1948 ◽  
Vol 67 (4) ◽  
pp. 552-556 ◽  
Author(s):  
W. C. Hess ◽  
E. H. Kramke ◽  
J. C. Fritz ◽  
H. W. Howard

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