Volume Change and Energy Dissipation in Rock Salt During Triaxial Failure Tests

Due to the gas injection and production of underground salt caves during the operational phase, rock salt is often subjected to a combined stress of cyclic pressure and constant pressure. In order to investigate the damage evolution of rock salt under different combined stresses, the uniaxial cyclic loading test and cyclic creep test were carried out. The stress-strain curves, energy characteristics, energy dissipation, and damage of rock salt in the two experiments were analyzed and compared. The test results show that the stress-strain curves of the two tests presented three stages of “sparse”-“dense”-“sparse.” As the maximum stress increases, the stage of “dense” will decrease and the rock salt cycle life will decrease. The relationship between cycle life and Δσ (difference between maximum and minimum stress in the tests) is an exponential function under cyclic loading and a linear relationship under cyclic creep. Based on the experimental data, the energy dissipation of rock salt is analyzed. The damage variables were defined from the perspective of energy dissipation, and the damage evolution of rock salt under two tests was obtained. There are three corresponding stages of energy dissipation and damage: initial, constant speed, and acceleration. The damage model is obtained by inverse functioning the s function, and then the correction coefficient is added to the model to obtain the modified damage model. The modified damage model is compared with the experimental data. The results show that the model can accurately describe the three stages of rock salt damage. The significance of parameters in the modifying damage model is also discussed.

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Artifacts caused by dehydration and epoxy embedding in TEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010008599x ◽

1991 ◽

Vol 49 ◽

pp. 338-339

Author(s):

Hilton H. Mollenhauer

Keyword(s):

Electron Microscopy ◽

Electron Beam ◽

Volume Change ◽

Tissue Damage ◽

Beam Specimen ◽

Chemical Fixation ◽

Resin Impregnation ◽

Storage Vesicles ◽

A Cell ◽

Tissue Shrinkage

Various means have been devised to preserve biological specimens for electron microscopy, the most common being chemical fixation followed by dehydration and resin impregnation. It is intuitive, and has been amply demonstrated, that these manipulations lead to aberrations of many tissue elements. This report deals with three parts of this problem: specimen dehydration, epoxy embedding resins, and electron beam-specimen interactions. However, because of limited space, only a few points can be summarized.Dehydration: Tissue damage, or at least some molecular transitions within the tissue, must occur during passage of a cell or tissue to a nonaqueous state. Most obvious, perhaps, is a loss of lipid, both that which is in the form of storage vesicles and that associated with tissue elements, particularly membranes. Loss of water during dehydration may also lead to tissue shrinkage of 5-70% (volume change) depending on the tissue and dehydrating agent.

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Short-term volume and turgor regulation in yeast

Essays in Biochemistry ◽

10.1042/bse0450147 ◽

2008 ◽

Vol 45 ◽

pp. 147-160 ◽

Cited By ~ 12

Author(s):

Jörg Schaber ◽

Edda Klipp

Keyword(s):

Dynamic Model ◽

Volume Change ◽

Volume Regulation ◽

Time Course ◽

Osmotic Shock ◽

Intracellular Concentration ◽

Short Term ◽

Hydrodynamic Property ◽

Turgor Regulation ◽

Thermodynamic Framework

Volume is a highly regulated property of cells, because it critically affects intracellular concentration. In the present chapter, we focus on the short-term volume regulation in yeast as a consequence of a shift in extracellular osmotic conditions. We review a basic thermodynamic framework to model volume and solute flows. In addition, we try to select a model for turgor, which is an important hydrodynamic property, especially in walled cells. Finally, we demonstrate the validity of the presented approach by fitting the dynamic model to a time course of volume change upon osmotic shock in yeast.

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