Differential Changes in Host Specificity among MGR586 DNA Fingerprint Groups of Pyricularia Grisea

2000 ◽  
pp. 234-242 ◽  
Author(s):  
J. C. Correll ◽  
T. L. Harp ◽  
J. C. Guerber ◽  
F. N. Lee
Keyword(s):  
Host Specificity ◽  
Dna Fingerprint ◽  
Pyricularia Grisea

scholarly journals Characterization of Pyricularia grisea in the United States Using Independent Genetic and Molecular Markers

2000 ◽  
Vol 90 (12) ◽  
pp. 1396-1404 ◽  
Author(s):  
J. C. Correll ◽  
T. L. Harp ◽  
J. C. Guerber ◽  
R. S. Zeigler ◽  
B. Liu ◽  
...  
Keyword(s):  
United States ◽  
Mating Type ◽  
Population Data ◽  
The United States ◽  
Vegetative Compatibility ◽  
Dna Fingerprint ◽  
Pyricularia Grisea ◽  
Vegetative Compatibility Groups ◽  
Large Numbers ◽  
Nit Mutants

A total of 540 isolates of Pyricularia grisea from rice in the United States were examined for vegetative compatibility, MGR586 DNA fingerprint diversity, and mating type based on hybridization with the mat1-1 and mat1-2 sexual mating type alleles. The collections contained both archived and contemporary field isolates representative of the known MGR586 lineages and races that occur throughout the United States. Complementary nitrate nonutilizing (nit) or sulfate nonutilizing (sul) mutants were used to assess vegetative compatibility in P. grisea. There was a complete correspondence between vegetative compatibility groups (VCGs), MGR586 lineage, and mating type among 527 contemporary isolates (collected between 1991 and 1997) from Arkansas, Louisiana, Missouri, Mississippi, and Texas; all isolates in MGR586 lineages A, B, C, and D belonged to VCGs US-01, US-02, US-03, and US-04, respectively. In addition, all isolates tested in VCGs US-01 and US-04 had the mat1-1 mating type allele whereas those in VCGs US-02 and US-03 had the mat1-2 allele. The strict association of independent markers during this sample period was consistent with a strictly asexual mode of reproduction. However, examination of archived isolates collected in the 1970s and 1980s and contemporary isolates revealed an incongruent relationship between the independent markers. MGR586 C and E isolates were vegetatively compatible which indicated that multiple robust MGR586 delineated lineages could be nested within certain VCGs. Although isolates in lineages C and E were vegetatively compatible, they were of opposite mating type. Several hypotheses, including recombination, could account for the incongruence between the various markers. Among the eight MGR586 lineages (A through H) that occur in the United States, all isolates in lineages A, D, E, G, and H had the mat1-1 allele, whereas isolates in lineages B, C, and F had the mat1-2 allele. Nit mutants can be recovered relatively easy from P. grisea and should allow large numbers of individuals within a population to be assessed for vegetative compatibility. VCGs may prove to be an effective multilocus marker in P. grisea. Thus, VCGs should be a useful means for characterizing genetic structure in populations of the rice blast fungus worldwide, provide a useful genetic framework to assist in interpreting molecular population data, and may provide insight into potential sexual or asexual recombination events.

scholarly journals Regional Population Diversity of Pyricularia grisea in Arkansas and the Influence of Host Selection

Plant Disease ◽  
2000 ◽  
Vol 84 (8) ◽  
pp. 877-884 ◽  
Author(s):  
J. Q. Xia ◽  
J. C. Correll ◽  
F. N. Lee ◽  
W. J. Ross ◽  
D. D. Rhoads
Keyword(s):  
Genetic Diversity ◽  
Dna Fingerprinting ◽  
Rice Blast ◽  
Nuclear Dna ◽  
The United States ◽  
Population Diversity ◽  
Dna Fingerprint ◽  
Pyricularia Grisea ◽  
Virulence Phenotype ◽  
Regional Population

MGR586 DNA fingerprinting has been widely used to characterize population diversity of the rice blast pathogen, Pyricularia grisea. However, the frequency and distribution of particular haplotypes (individuals) within MGR-delimited lineages has not been examined in the United States. MGR586 DNA fingerprinting, mitochondrial DNA (mtDNA) restriction fragment length polymorphism (RFLPs), and virulence phenotyping were used to examine genetic diversity of P. grisea in Arkansas. A total of 470 monoconidial isolates were recovered from eight rice cultivars in 18 commercial fields in nine counties in Arkansas. All isolates were examined for nuclear DNA RFLPs with the MGR586 DNA fingerprint probe, and both the MGR lineage (isolates with >80% similarity) and the haplotype frequencies were determined. Four distinct MGR586 DNA fingerprint lineages (designated A, B, C, and D) were identified among the 470 field isolates. All four lineages were found in 9 of the 18 locations. Three lineages were found in four locations, two lineages in three locations, and only a single lineage was found at two locations. In all, 10, 19, 16, and 13 haplotypes (isolates which had MGR586 DNA fingerprints which differed by 1 to 20%) were identified within lineages A, B, C, and D, respectively, among the 470 isolates examined. Within each lineage, a single haplotype (clone) predominated, representing 51 to 71% of the isolates collected for each of the four lineages. Overall, 60% of the 470 isolates belonged to one of only four haplotypes (A1, B1, C1, and D1) and these four predominant haplotypes were recovered from between 7 and 14 of the 18 locations sampled, indicating a widespread distribution of these four clones. These data indicate an exceptionally low level of genetic diversity in the regional rice blast pathogen population in Arkansas relative to several other populations of P. grisea examined from tropical environments. In addition, no mtDNA RFLPs were detected among representative haplotypes within each of the lineages, indicating a single mtDNA haplotype was present in the population. Examination of virulence indicated that two races predominated in the regional collection. All 30 isolates in lineages A and C tested had an IB-49 virulence phenotype. Out of 30 isolates in lineages B and D, 29 had an IC-17 virulence phenotype. One isolate in lineage B, isolated from a highly susceptible cultivar (L201), had an IG-1 virulence phenotype. The frequencies of the four lineages varied among the locations sampled and may have been due, in part, to the cultivar from which isolates were recovered. A single lineage was recovered from two cultivars, Mars and Millie. Although only a single field of each of these cultivars was sampled, the data indicate that certain cultivars grown in Arkansas may serve as a “bottleneck”, selecting out specific lineages in the regional population. To test this hypothesis, an additional 283 isolates were recovered from replicated plots of cvs. M204 and Mars located within commercial rice fields at two locations during two seasons. All four MGR586 lineages were recovered from each location. However, there was a strong bias for lineage B on cv. M204 (79% of all isolates) and a strong bias for lineage A on cv. Mars (95% of all isolates), indicating some cultivars were effective in excluding certain lineages.

scholarly journals Pathotype and Avirulence Gene Diversity of Pyricularia grisea in Thailand as Determined by Rice Lines Near-Isogenic for Major Resistance Genes

Plant Disease ◽  
2000 ◽  
Vol 84 (1) ◽  
pp. 60-70 ◽  
Author(s):  
Poonsak Mekwatanakarn ◽  
Wichai Kositratana ◽  
M. Levy ◽  
R. S. Zeigler
Keyword(s):  
Resistance Genes ◽  
Virulence Genes ◽  
Gene Diversity ◽  
Dna Fingerprint ◽  
Avirulence Gene ◽  
Pyricularia Grisea ◽  
Recurrent Parent ◽  
Rice Cultivars ◽  
Study Region ◽  
Major Resistance Genes

Five hundred twenty-seven isolates of Pyricularia grisea were collected from trap rice cultivars of indigenous and exotic origin across three seasons at five sites in Thailand. Single conidium isolates were inoculated onto 15 rice lines near-isogenic (NILs) for resistance genes, one recurrent parent, and two local cultivars. One hundred seventy-five pathotypes were identified, of which 160 were represented by fewer than eight isolates. Predicted pathotype number was estimated at greater than 450 for the study region. Significant differences in pathotype diversity were detected across sites, seasons, and among isolates collected from exotic versus indigenous hosts. Isolates and pathotypes with greater numbers of virulence genes (as inferred from compatibility with NILs) were less common than those with fewer virulence genes. Analysis of virulence distributions among isolates grouped according to their MGR586 DNA-fingerprint similarities (i.e., “lineages”) also showed that, for the most commonly represented lineages, isolates with fewer virulence genes predominated. Lineages represented by one or a few isolates had greater numbers of virulence genes. Lower frequency of recovery of isolates with accumulated virulence genes is consistent with an associated fitness penalty. Resistance genes Pi 1, Pi z-5, and Pi ta2 were broadly effective across this population, compatibility with Pi 1 and Pi z-5 was very rare, and no isolate combined compatibility with both genes. Well-represented (more than 20 isolates) MGR586 lineages showed specific incompatibilities with some NILs, but these were restricted to Pi 1 and Pi z-5. No combination of resistance genes would confer resistance across all lineages.

Classification and host specificity of Metagonimus spp. from Korean freshwater fish

1996 ◽  
Vol 34 (1) ◽  
pp. 7 ◽  
Author(s):  
H J Rim ◽  
K H Kim ◽  
K H Joo
Keyword(s):  
Freshwater Fish ◽  
Host Specificity

Alteration of host specificity in Bacillus subtilis.

1968 ◽  
Vol 32 (4_Pt_1) ◽  
pp. 297-301 ◽  
Author(s):  
D D Gwinn ◽  
W D Lawton
Keyword(s):  
Bacillus Subtilis ◽  
Host Specificity

An ecological study of the branchiobdellid commensals (Annelida-Branchiobdellidae) of some mid-western Ontario crayfish

1968 ◽  
Vol 46 (5) ◽  
pp. 835-843 ◽  
Author(s):  
John E. Bishop
Keyword(s):  
Host Specificity ◽  
Dominant Species ◽  
Ecological Study ◽  
Rabbit Serum ◽  
First Year ◽  
Serological Testing ◽  
Severe Winter ◽  
Orconectes Propinquus ◽  
Winter Conditions ◽  
Laurel Creek

Orconectes propinquus and Cambarus robustus from the Speed River, Sunfish Lake, and Laurel Creek, harbor two branchiobdellids, Cambarincola chirocephala and Pterodrilus distichus. Both adult and cocoon populations of the dominant species (C. chirocephala) are proportional to the size of the host throughout the year, except that first-year crayfish are free of cocoons. The reduction in total number of commensals from autumn to spring can be attributed to severe winter conditions. A subrostral site of preference for adult branchiobdellid attachment, and a dominant abdomen I and II site for cocoon deposition are indicated for O. propinquus. On C. robustus, adults are most commonly found on the antennal bases and among the maxillipeds, and cocoons on the last live abdominal sternites. No host specificity is evident although an unidentified Cambarus sp. from Sunfish Lake is free of commensals. Host incompatibility may explain this, but data from Laurel Creek indicate that silting of the microhabitat is responsible for loss of branchiobdellid population. The crayfish–branchiobdellid relationship is commensal, or at most facultatively parasitic, as adult worms can live without a host for extended periods. Serological testing of rabbit serum containing branchiobdellid antibodies against crayfish serum is negative. The dependence of the egg stage on the host for some undetermined factor or factors is discussed. An Asellus sp. fails to pick up the commensals even when exposed under ideal conditions for colonization.

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