Identification of DNA Markers Linked to Partial Resistance for Blast Disease in Rice Across Four Locations

2000 ◽  
pp. 34-42 ◽  
Author(s):  
Prashanth G. Bagali ◽  
Shailaja Hittalmani ◽  
Srinivasachary Y. Shashidhar ◽  
H. E. Shashidhar
Keyword(s):  
Dna Markers ◽  
Partial Resistance ◽  
Blast Disease

Assessment of partial resistance to rice blast disease

2018 ◽  
Vol 55 (3) ◽  
pp. 363
Author(s):  
AK Mukherjee ◽  
NK Mohapatra ◽  
P Nayak
Keyword(s):  
Partial Resistance ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease

scholarly journals Resistance Genes and Selection DNA Markers for Blast Disease in Rice (Oryza sativa L.)

2009 ◽  
Vol 43 (4) ◽  
pp. 255-280 ◽  
Author(s):  
Yohei KOIDE ◽  
Nobuya KOBAYASHI ◽  
Donghe XU ◽  
Yoshimichi FUKUTA
Keyword(s):  
Oryza Sativa ◽  
Resistance Genes ◽  
Dna Markers ◽  
Oryza Sativa L ◽  
Blast Disease

scholarly journals RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar.

Genetics ◽  
1994 ◽  
Vol 136 (4) ◽  
pp. 1421-1434 ◽  
Author(s):  
G L Wang ◽  
D J Mackill ◽  
J M Bonman ◽  
S R McCouch ◽  
M C Champoux ◽  
...  
Keyword(s):  
Partial Resistance ◽  
Blast Resistance ◽  
Durable Resistance ◽  
Complex Trait ◽  
Rice Cultivar ◽  
Blast Disease ◽  
Rflp Markers ◽  
Japonica Rice ◽  
Qualitative Resistance ◽  
Ri Lines

Abstract Moroberekan, a japonica rice cultivar with durable resistance to blast disease in Asia, was crossed to the highly susceptible indica cultivar, CO39, and 281 F7 recombinant inbred (RI) lines were produced by single seed descent. The population was evaluated for blast resistance in the greenhouse and the field, and was analyzed with 127 restriction fragment length polymorphism (RFLP) markers. Two dominant loci associated with qualitative resistance to five isolates of the fungus were tentatively named Pi-5(t) and Pi-7(t). They were mapped on chromosomes 4 and 11, respectively. To identify quantitative trait loci (QTLs) affecting partial resistance, RI lines were inoculated with isolate PO6-6 of Pyricularia oryzae in polycyclic tests. Ten chromosomal segments were found to be associated with effects on lesion number (P < 0.0001 and LOD > 6.0). Three of the markers associated with QTLs for partial resistance had been reported to be linked to complete blast resistance in previous studies. QTLs identified in greenhouse tests were good predictors of blast resistance at two field sites. This study illustrates the usefulness of RI lines for mapping a complex trait such as blast resistance and suggests that durable resistance in the traditional variety, Moroberekan, involves a complex of genes associated with both partial and complete resistance.

scholarly journals Identification of a Pi9-Containing Rice Germplasm with a Newly Developed Robust Marker

2016 ◽  
Vol 106 (8) ◽  
pp. 871-876 ◽  
Author(s):  
Klaus Konrad Scheuermann ◽  
Yulin Jia
Keyword(s):  
Dna Markers ◽  
Sequence Variation ◽  
Blast Resistance ◽  
Blast Disease ◽  
Rice Germplasm ◽  
Oryza Minuta ◽  
Dna Sequence Variation ◽  
Aa Genome ◽  
Dna Primers ◽  
New Cultivars

The Pi9 gene in rice, originating from Oryza minuta, is an effective resistance gene for controlling rice blast disease. However, currently available linked DNA markers do not accurately identify the function of Pi9, thus hindering its efficient incorporation into new cultivars through marker-assisted selection (MAS). In addition, no known Pi9-containing rice germplasm is available to breeders. In the present study, DNA sequence variation of Pi9 alleles and their family members was analyzed in 40 diverse rice germplasm accessions from the AA genome to develop a robust Pi9 marker. In total, 29 DNA primers of 20 to 23 nucleotides were designed and each possible combination of primer pairs was used to detect Pi9. Only one combination of DNA primers, KS28/KS6, was identified to specifically detect Pi9 in the monogenic line IRBL9-W. The presence of Pi9 was verified with the predicted Pi9-specific blast reaction. Subsequently, 201 genetically diverse mini-core rice accessions from 114 countries were screened with KS28/KS6. One germplasm, IR 9660-48-1-1-2, was identified to carry Pi9 and the function of Pi9 was verified with pathogenicity assays. This robust Pi9 marker and a rice germplasm, IR9660-48-1-1-2 (GSOR310687), carrying Pi9 can be used to improve blast resistance with a MAS approach.

Creation of blast disease-resistant rice varieties with modern DNA-markers

2015 ◽  
Vol 51 (8) ◽  
pp. 752-756 ◽  
Author(s):  
E. V. Dubina ◽  
Zh. M. Mukhina ◽  
E. M. Kharitonov ◽  
V. N. Shilovskiy ◽  
E. S. Kharchenko ◽  
...  
Keyword(s):  
Dna Markers ◽  
Blast Disease ◽  
Rice Varieties ◽  
Disease Resistant

Sex Differences in Childhood Associations between DNA Markers and General Cognitive Ability

2007 ◽  
Vol 28 (3) ◽  
pp. 161-164 ◽  
Author(s):  
Rosalind Arden ◽  
Nicole Harlaar ◽  
Robert Plomin
Keyword(s):  
Sex Differences ◽  
Single Nucleotide Polymorphisms ◽  
Cognitive Ability ◽  
Dna Markers ◽  
Community Sample ◽  
Nucleotide Polymorphisms ◽  
General Cognitive Ability ◽  
Single Nucleotide ◽  
The Difference

Abstract. An association between intelligence at age 7 and a set of five single-nucleotide polymorphisms (SNPs) has been identified and replicated. We used this composite SNP set to investigate whether the associations differ between boys and girls for general cognitive ability at ages 2, 3, 4, 7, 9, and 10 years. In a longitudinal community sample of British twins aged 2-10 (n > 4,000 individuals), we found that the SNP set is more strongly associated with intelligence in males than in females at ages 7, 9, and 10 and the difference is significant at 10. If this finding replicates in other studies, these results will constitute the first evidence of the same autosomal genes acting differently on intelligence in the two sexes.

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