USING DNA MARKERS ENCODING P24 AND GP51 PROTEINS IN PCR-RT TO INCREASE SENSITIVITY AND SPECIFICITY OF THE PCR METHOD FOR INDICATION OF BLV

2019 ◽  
pp. 8-14
Author(s):  
R.F. Safina ◽  
◽  
G.R. Lukmanova ◽  
K.V. Usoltsev ◽  
N.I. Khammadov ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Dna Markers ◽  
Pcr Method

scholarly journals Genotyping of Strawberry (Fragaria ananassa Duch.) Cultivars by DNA Markers: Interlaboratory Study

2009 ◽  
Vol 92 (3) ◽  
pp. 896-906 ◽  
Author(s):  
Miyuki Kunihisa ◽  
Hiroshi Ueda ◽  
Nobuko Fukino ◽  
Satoru Matsumoto ◽  
T Akasaki ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Dna Markers ◽  
Interlaboratory Study ◽  
Fragaria Ananassa ◽  
Statistical Validation ◽  
First Report ◽  
Caps Markers ◽  
Strawberry Cultivars ◽  
Specificity Rate

Abstract Fourteen Japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (CAPS) markers for discrimination of strawberry (Fragaria ananassa Duch.) cultivars. Both the sensitivity and specificity rate of 12 markers were 100, those of another 12 were >95, and those of 1 were >90. These results indicate that the method of genotyping by the CAPS markers was highly reproducible and could provide a useful basis for practical identification of strawberry cultivars. This is the first report of the statistical validation of crop genotyping by DNA markers.

Bayesian analysis of culture and PCR methods for detection of Campylobacter spp. in broiler caecal samples

2014 ◽  
Vol 143 (2) ◽  
pp. 298-307 ◽  
Author(s):  
M. E. ARNOLD ◽  
E. M. JONES ◽  
J. R. LAWES ◽  
A. B. VIDAL ◽  
F. A. CLIFTON-HADLEY ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Gold Standard ◽  
Culture Method ◽  
Mixed Population ◽  
Total Probability ◽  
Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Parallel Sampling ◽  
Campylobacter Spp

SUMMARYThe objective of this study was to estimate the sensitivity and specificity of a culture method and a polymerase chain reaction (PCR) method for detection of two Campylobacter species: C. jejuni and C. coli. Data were collected during a 3-year survey of UK broiler flocks, and consisted of parallel sampling of caeca from 436 batches of birds by both PCR and culture. Batches were stratified by season (summer/non-summer) and whether they were the first depopulation of the flock, resulting in four sub-populations. A Bayesian approach in the absence of a gold standard was adopted, and the sensitivity and specificity of the PCR and culture for each Campylobacter subtype was estimated, along with the true C. jejuni and C. coli prevalence in each sub-population. Results indicated that the sensitivity of the culture method was higher than that of PCR in detecting both species when the samples were derived from populations infected with at most one species of Campylobacter. However, from a mixed population, the sensitivity of culture for detecting both C. jejuni or C. coli is reduced while PCR is potentially able to detect both species, although the total probability of correctly identifying at least one species by PCR is similar to that of the culture method.

scholarly journals Bayesian estimation of sensitivity and specificity of a PCR method to detect Coxiella burnetii in milk and vaginal secretions in sheep and goat samples

2019 ◽  
Vol 102 (6) ◽  
pp. 4954-4959 ◽  
Author(s):  
Zeinab Abiri ◽  
Mohammad Khalili ◽  
Polycronis Kostoulas ◽  
Hamid Sharifi ◽  
Mehrnaz Rad ◽  
...  
Keyword(s):  
Bayesian Estimation ◽  
Sensitivity And Specificity ◽  
Coxiella Burnetii ◽  
Vaginal Secretions ◽  
Pcr Method

scholarly journals Evaluation of a Commercial PCR Kit for Diagnosis of Cytomegalovirus Infection of the Central Nervous System

1998 ◽  
Vol 36 (11) ◽  
pp. 3382-3384 ◽  
Author(s):  
Adriana Weinberg ◽  
Debbie Spiers ◽  
Guan-Yung Cai ◽  
Christopher M. Long ◽  
Rita Sun ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Sensitivity And Specificity ◽  
Clinical Setting ◽  
Cytomegalovirus Infection ◽  
The Central Nervous System ◽  
Pcr Method

We evaluated the AMPLICOR cytomegalovirus (CMV) PCR kit for the diagnosis of neurologic CMV infections on 43 positive and 112 negative archived cerebrospinal fluid specimens originally tested by an in-house PCR method. The AMPLICOR kit showed sensitivity and specificity of 95 and 100%, respectively, versus the home-grown assay, indicating its utility in this clinical setting.

scholarly journals Specialisation of Rhynchosporium secalis (Oud.) J.J. Davis infecting barley and rye

2010 ◽  
Vol 42 (No. 3) ◽  
pp. 85-93 ◽  
Author(s):  
L. Lebedeva ◽  
L. Tvarůžek
Keyword(s):  
Hordeum Vulgare ◽  
Secale Cereale ◽  
Dna Markers ◽  
Barley Variety ◽  
Effect Of Temperature ◽  
Rhynchosporium Secalis ◽  
Media Effect ◽  
Nutrient Media ◽  
Rapd Pcr ◽  
Pcr Method

Fifty-five isolates of <i>Rhynchosporium secalis</i> from <i>Hordeum vulgare</i> and 34 isolates from Secale cereale were compared for growth on different nutrient media, effect of temperature on growth and morphology of colonies. The pathogenicity of the isolates was assessed on 10 rye varieties, 10 triticale varieties and the susceptible barley variety Gambrinus. The triticale varieties differed in the number of rye chromosomes in the genome. Isozymes of <i>R. secalis</i> isolated from infected leaves of barley and rye were compared. The RAPD-PCR method was used for comparison of isolates on DNA-markers. The analysis indicated two specialised forms of the fungus; each of them able to develop only on its original host.

Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato

Euphytica ◽  
2011 ◽  
Vol 180 (3) ◽  
pp. 347-355 ◽  
Author(s):  
Kazuyuki Mori ◽  
Yu Sakamoto ◽  
Nobuhiro Mukojima ◽  
Seiji Tamiya ◽  
Takashi Nakao ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Resistance Genes ◽  
Dna Markers ◽  
Simultaneous Detection ◽  
Pest Resistance ◽  
Pcr Method

scholarly journals A revised its nucleotide sequence gives a specifity for Smallanthus sonchifolius (Poepp. and Endl.) and its products identification

Genetika ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 217-226 ◽  
Author(s):  
Jana Ziarovská ◽  
Eloy Fernández ◽  
Luigi Millela
Keyword(s):  
Nucleotide Sequence ◽  
Restriction Endonuclease ◽  
Dna Markers ◽  
Unique Sequence ◽  
The Other ◽  
Its Sequence ◽  
Smallanthus Sonchifolius ◽  
Its Regions ◽  
Pcr Method ◽  
Specific Food

Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its benefits for people suffering from diabetes or various digestive or renal disorders. Because no specific Smallanthus sonchifolius identification DNA markers are still known the paper demonstrates ITS regions to be able to detect and differentiate among yacon species and the potential for specific food authentification purposes is reported, too. The newly sequenced ITS of yacon accessions originated in Peru, Ecuador and Bolivia analyse provide the unique sequence site that differs from all of the other yacon species and is recognized by DraIII restriction endonuclease. Restriction cleavadge of the PCR amplified ITSs of the twenty-eight yacon accessions was performed and in all cases the recognition site was confirmed as a typical for Smallanthus sonchifolius . Based on the nucleotide specifity of Smallanthus sonchifolius, ITS sequence the PCR method combined with the restriction clevadge protocol was developed for yacon identification.

scholarly journals Evaluation of an Antibody-Based Test for the Diagnosis of Chikungunya Infection

2020 ◽  
Vol 7 (2) ◽  
pp. 68-72
Author(s):  
Mohammad Tanvir Islam ◽  
Afzalun Nessa ◽  
Sharmin Sultana ◽  
Mohammad Rafiqul Islam ◽  
Mohammad Ferdous Ur Rahaman ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Acute Phase ◽  
Antibody Test ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Igm Antibody ◽  
Clinical Presentations ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Medical University

Background: The diagnosis of chikungunya is confusing due to similar clinical presentations of different viral illnesses. Objective: The purpose of the present study was to evaluate the accuracy of an antibody-based test for the diagnosis of chikungunya infection Methodology: This cross-sectional study was conducted at the Department of Internal Medicine, Bangabandhu Sheikh Mujib Medical University, Dhaka, from July to September 2017 when an outbreak of chikungunya occurred in Bangladesh. Chikungunya patients were evaluated by the IgM antibody test by immunochromatographic method (ICT) during both the early phase and the convalescent-phase. The sensitivity and specificity of the IgM antibody test were estimated considering the polymerase chain reaction (PCR) method as the gold standard. Result: The sensitivity and specificity of the IgM antibody test in the acute phase were 2.7 percent and 79.5 percent, respectively. In contrast, in the convalescence phase, sensitivity and specificity were 86.5 percent and 33.3 percent. Conclusion: Antibody-based testing was found not suitable for detecting chikungunya infection during the acute phase of the illness. Journal of Current and Advance Medical Research 2020;7(2): 68-72

Sign in / Sign up

Export Citation Format

Share Document