The paper presents data on total and faecal coliform removal and on intestinal nematode egg removal achieved in the Al-Samra stabilization ponds in Jordan during the winter period December 1986 to March 1987. A total retention of 34 days in the series of 10 ponds was able to achieve complete removal of nematode eggs at the pond temperature 12-15°C but was not able to meet the WHO guideline value for faecal conforms of ≤ 1000/100 ml. In addition to retention time having a positive effect on faecal conform die-off, it was shown that surface organic loading, pond pH, BOD5 concentration, and depth, influenced the rate of faecal coliform die-off. It became apparent that interactions existed among the operational and environmental parameters but that the simple time-and-temperature-dependent model for faecal conform die-off was no longer appropriate.